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28S junctions and chimeric elements of the rDNA targeting non-LTR retrotransposon R2 in crustacean living fossils (Branchiopoda, Notostraca).
Genomics 2012; 100(1):51-6G

Abstract

The 28S rRNA genes of several metazoans are interrupted by site-specific targeting non-LTR retrotransposons, such as R2. R2 elements have been deeply analyzed but aspects of their retrotransposition mechanism and the origin of the wide diversity observed are still debated. We characterized six new R2 lineages in four tadpole shrimp species (Notostraca), samples deriving from a parthenogenetic population of Triops cancriformis (R2Tc_it) and from bisexual Lepidurus populations of L. lubbocki (R2Ll), L. couesii (R2LcA, R2LcB, R2LcC) and L. arcticus (R2La). All elements fit the canonical R2 structure but R2Ll which turned out to be a chimera with an additional ORF originating from another R2. Consistently with data on LINEs, R2Ll could be the result of recombination due to reverse transcriptase template jump. The analysis of 28S/R2 5' end junctions further suggests aberrant homologous recombination, as observed in RNA viruses.

Authors+Show Affiliations

Dip. Biologia Evoluzionistica Sperimentale, Università di Bologna, via Selmi 3, 40126 Bologna, Italy. andrea.luchetti@unibo.itNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22564473

Citation

Luchetti, Andrea, et al. "28S Junctions and Chimeric Elements of the rDNA Targeting non-LTR Retrotransposon R2 in Crustacean Living Fossils (Branchiopoda, Notostraca)." Genomics, vol. 100, no. 1, 2012, pp. 51-6.
Luchetti A, Mingazzini V, Mantovani B. 28S junctions and chimeric elements of the rDNA targeting non-LTR retrotransposon R2 in crustacean living fossils (Branchiopoda, Notostraca). Genomics. 2012;100(1):51-6.
Luchetti, A., Mingazzini, V., & Mantovani, B. (2012). 28S junctions and chimeric elements of the rDNA targeting non-LTR retrotransposon R2 in crustacean living fossils (Branchiopoda, Notostraca). Genomics, 100(1), pp. 51-6. doi:10.1016/j.ygeno.2012.04.005.
Luchetti A, Mingazzini V, Mantovani B. 28S Junctions and Chimeric Elements of the rDNA Targeting non-LTR Retrotransposon R2 in Crustacean Living Fossils (Branchiopoda, Notostraca). Genomics. 2012;100(1):51-6. PubMed PMID: 22564473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 28S junctions and chimeric elements of the rDNA targeting non-LTR retrotransposon R2 in crustacean living fossils (Branchiopoda, Notostraca). AU - Luchetti,Andrea, AU - Mingazzini,Valentina, AU - Mantovani,Barbara, Y1 - 2012/05/01/ PY - 2012/03/22/received PY - 2012/04/17/revised PY - 2012/04/23/accepted PY - 2012/5/9/entrez PY - 2012/5/9/pubmed PY - 2012/10/27/medline SP - 51 EP - 6 JF - Genomics JO - Genomics VL - 100 IS - 1 N2 - The 28S rRNA genes of several metazoans are interrupted by site-specific targeting non-LTR retrotransposons, such as R2. R2 elements have been deeply analyzed but aspects of their retrotransposition mechanism and the origin of the wide diversity observed are still debated. We characterized six new R2 lineages in four tadpole shrimp species (Notostraca), samples deriving from a parthenogenetic population of Triops cancriformis (R2Tc_it) and from bisexual Lepidurus populations of L. lubbocki (R2Ll), L. couesii (R2LcA, R2LcB, R2LcC) and L. arcticus (R2La). All elements fit the canonical R2 structure but R2Ll which turned out to be a chimera with an additional ORF originating from another R2. Consistently with data on LINEs, R2Ll could be the result of recombination due to reverse transcriptase template jump. The analysis of 28S/R2 5' end junctions further suggests aberrant homologous recombination, as observed in RNA viruses. SN - 1089-8646 UR - https://www.unboundmedicine.com/medline/citation/22564473/28S_junctions_and_chimeric_elements_of_the_rDNA_targeting_non_LTR_retrotransposon_R2_in_crustacean_living_fossils__Branchiopoda_Notostraca__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0888-7543(12)00064-X DB - PRIME DP - Unbound Medicine ER -