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Effects of heavy metals on ultrastructure and HSP70s induction in the aquatic moss Leptodictyum riparium Hedw.
Int J Phytoremediation. 2012 Apr; 14(4):443-55.IJ

Abstract

The effects of heavy metals, both toxic (Pb, Cd) and essential (Cu, Zn) on the ultrastructure and the induction of Heat Shock Protein 70 (HSP70) have been studied in the aquatic moss Leptodictyum riparium Hedw. In vitro cultured L. riparium was treated with different heavy metals, both toxic, as cadmium or lead; and essential microelements such as Copper or Zinc concentrations ranging from 10(-3) to 10(-6) M to investigate both ultrastructural damage and HSP induction. TEM observations showed that sub-lethal concentrations of heavy metals caused only slight changes, largely localized in the chloroplasts. Among all the heavy metals tested, cadmium caused the most severe modifications. Heavy metals caused the decrease of the soluble protein content and the enhancement of proteins reacting versus HSP70 antibodies, suggesting that molecular chaperons might be involved in the resistance to toxic effects of lead, cadmium, copper and zinc. Therefore, the induction of HSP70 in L. riparium would confer a higher resistance to pollutants under stressful conditions lethal for other mosses and higher plant species. These results suggest that the moss L. riparium can tolerate heavy metals stress without incurring severe cellular/subcellular damage. Therefore it can be used as a useful indicator of heavy metals accumulation.

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Authors+Show Affiliations

Esposito S
Dipartimento di Biologia Strutturale e Funzionale - Università di Napoli "Federico II" - Naples, Italy. sergio.esposito@unina.it
Sorbo S
No affiliation info available
Conte B
No affiliation info available
Basile A
No affiliation info available

MeSH

BryopsidaCadmiumCopperElectron Probe MicroanalysisEnvironmental PollutantsHSP70 Heat-Shock ProteinsLeadMetals, HeavyMicroscopy, Electron, TransmissionZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22567723

Citation

Esposito, S, et al. "Effects of Heavy Metals On Ultrastructure and HSP70s Induction in the Aquatic Moss Leptodictyum Riparium Hedw." International Journal of Phytoremediation, vol. 14, no. 4, 2012, pp. 443-55.
Esposito S, Sorbo S, Conte B, et al. Effects of heavy metals on ultrastructure and HSP70s induction in the aquatic moss Leptodictyum riparium Hedw. Int J Phytoremediation. 2012;14(4):443-55.
Esposito, S., Sorbo, S., Conte, B., & Basile, A. (2012). Effects of heavy metals on ultrastructure and HSP70s induction in the aquatic moss Leptodictyum riparium Hedw. International Journal of Phytoremediation, 14(4), 443-55.
Esposito S, et al. Effects of Heavy Metals On Ultrastructure and HSP70s Induction in the Aquatic Moss Leptodictyum Riparium Hedw. Int J Phytoremediation. 2012;14(4):443-55. PubMed PMID: 22567723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of heavy metals on ultrastructure and HSP70s induction in the aquatic moss Leptodictyum riparium Hedw. AU - Esposito,S, AU - Sorbo,S, AU - Conte,B, AU - Basile,A, PY - 2012/5/10/entrez PY - 2012/5/10/pubmed PY - 2014/5/23/medline SP - 443 EP - 55 JF - International journal of phytoremediation JO - Int J Phytoremediation VL - 14 IS - 4 N2 - The effects of heavy metals, both toxic (Pb, Cd) and essential (Cu, Zn) on the ultrastructure and the induction of Heat Shock Protein 70 (HSP70) have been studied in the aquatic moss Leptodictyum riparium Hedw. In vitro cultured L. riparium was treated with different heavy metals, both toxic, as cadmium or lead; and essential microelements such as Copper or Zinc concentrations ranging from 10(-3) to 10(-6) M to investigate both ultrastructural damage and HSP induction. TEM observations showed that sub-lethal concentrations of heavy metals caused only slight changes, largely localized in the chloroplasts. Among all the heavy metals tested, cadmium caused the most severe modifications. Heavy metals caused the decrease of the soluble protein content and the enhancement of proteins reacting versus HSP70 antibodies, suggesting that molecular chaperons might be involved in the resistance to toxic effects of lead, cadmium, copper and zinc. Therefore, the induction of HSP70 in L. riparium would confer a higher resistance to pollutants under stressful conditions lethal for other mosses and higher plant species. These results suggest that the moss L. riparium can tolerate heavy metals stress without incurring severe cellular/subcellular damage. Therefore it can be used as a useful indicator of heavy metals accumulation. SN - 1522-6514 UR - https://www.unboundmedicine.com/medline/citation/22567723/Effects_of_heavy_metals_on_ultrastructure_and_HSP70s_induction_in_the_aquatic_moss_Leptodictyum_riparium_Hedw_ L2 - https://www.tandfonline.com/doi/full/10.1080/15226514.2011.620904 DB - PRIME DP - Unbound Medicine ER -