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Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®.
Pharm Biol. 2012 Jun; 50(6):720-6.PB

Abstract

CONTEXT

Polyherbal formulations containing different plants are used for the treatment of various diseases. Romix® powder is a polyherbal formulation consisting of 14 traditionally used herbs and is used as a food supplement. There is no information about pharmaceutical activities of Romix®.

OBJECTIVE

This study determined the total phenolic and total flavonoid content, and investigated the antioxidant and anti-inflammatory activities and acute toxicity of Romix®.

MATERIAL AND METHODS

The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteu reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The quercetin content of the extract was analyzed using the high-performance liquid chromatography (HPLC) method. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the rat.

RESULTS

The flavonoid and phenolics contents of Romix® were 50.58 and 265.83 mg/g in ethanol extract and 18.60 and 222.50 mg/g in water extract, respectively. Total quercetin content of Romix® was determined as 2.857 mg/g. Antioxidant activity results showed that ethanol extract in 1 mg/mL concentration (4.49775 µg/mL) had moderate antioxidant activity than water extract in the same concentration (4.28191 µg/mL). Intraperitoneal administration of 25 mg/kg Romix® extract exhibited anti-inflammatory activity and inhibited paw swelling at 1, 2, 3, 4, 5 and 6 h in rats with no acute toxicity.

CONCLUSION

These findings suggest that Romix® due to its antioxidant and anti-inflammatory activities may be useful in the prevention or treatment of aging-related and inflammatory diseases.

Authors+Show Affiliations

Center for Drug Research & Development and Pharmacokinetic Applications, Ege University, Izmir, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22571398

Citation

Ince, Iskender, et al. "Antioxidant, Anti-inflammatory Activities and Acute Toxicity of the Polyherbal Formulation: Romix®." Pharmaceutical Biology, vol. 50, no. 6, 2012, pp. 720-6.
Ince I, Kayalar H, Elgin G, et al. Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®. Pharm Biol. 2012;50(6):720-6.
Ince, I., Kayalar, H., Elgin, G., Koksal, C., & Yavasoglu, N. U. (2012). Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®. Pharmaceutical Biology, 50(6), 720-6. https://doi.org/10.3109/13880209.2011.622287
Ince I, et al. Antioxidant, Anti-inflammatory Activities and Acute Toxicity of the Polyherbal Formulation: Romix®. Pharm Biol. 2012;50(6):720-6. PubMed PMID: 22571398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, anti-inflammatory activities and acute toxicity of the polyherbal formulation: Romix®. AU - Ince,Iskender, AU - Kayalar,Husniye, AU - Elgin,Gozde, AU - Koksal,Cinel, AU - Yavasoglu,N Ulku Karabay, PY - 2012/5/11/entrez PY - 2012/5/11/pubmed PY - 2012/9/25/medline SP - 720 EP - 6 JF - Pharmaceutical biology JO - Pharm Biol VL - 50 IS - 6 N2 - CONTEXT: Polyherbal formulations containing different plants are used for the treatment of various diseases. Romix® powder is a polyherbal formulation consisting of 14 traditionally used herbs and is used as a food supplement. There is no information about pharmaceutical activities of Romix®. OBJECTIVE: This study determined the total phenolic and total flavonoid content, and investigated the antioxidant and anti-inflammatory activities and acute toxicity of Romix®. MATERIAL AND METHODS: The total phenolics in the extracts were determined colorimetrically by using the Folin-Ciocalteu reagent. The total flavonoid content of the extracts was evaluated by a spectrophotometric method. The quercetin content of the extract was analyzed using the high-performance liquid chromatography (HPLC) method. Antioxidant activity of the extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assays. The anti-inflammatory activity was evaluated by the carrageenan-induced paw edema test in the rat. RESULTS: The flavonoid and phenolics contents of Romix® were 50.58 and 265.83 mg/g in ethanol extract and 18.60 and 222.50 mg/g in water extract, respectively. Total quercetin content of Romix® was determined as 2.857 mg/g. Antioxidant activity results showed that ethanol extract in 1 mg/mL concentration (4.49775 µg/mL) had moderate antioxidant activity than water extract in the same concentration (4.28191 µg/mL). Intraperitoneal administration of 25 mg/kg Romix® extract exhibited anti-inflammatory activity and inhibited paw swelling at 1, 2, 3, 4, 5 and 6 h in rats with no acute toxicity. CONCLUSION: These findings suggest that Romix® due to its antioxidant and anti-inflammatory activities may be useful in the prevention or treatment of aging-related and inflammatory diseases. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/22571398/Antioxidant_anti_inflammatory_activities_and_acute_toxicity_of_the_polyherbal_formulation:_Romix®_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2011.622287 DB - PRIME DP - Unbound Medicine ER -