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Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice.
Biomed Res 2012; 33(2):119-32BR

Abstract

Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day- to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.

Authors+Show Affiliations

Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22572386

Citation

Quispe-Salcedo, Angela, et al. "Expression Patterns of Nestin and Dentin Sialoprotein During Dentinogenesis in Mice." Biomedical Research (Tokyo, Japan), vol. 33, no. 2, 2012, pp. 119-32.
Quispe-Salcedo A, Ida-Yonemochi H, Nakatomi M, et al. Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice. Biomed Res. 2012;33(2):119-32.
Quispe-Salcedo, A., Ida-Yonemochi, H., Nakatomi, M., & Ohshima, H. (2012). Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice. Biomedical Research (Tokyo, Japan), 33(2), pp. 119-32.
Quispe-Salcedo A, et al. Expression Patterns of Nestin and Dentin Sialoprotein During Dentinogenesis in Mice. Biomed Res. 2012;33(2):119-32. PubMed PMID: 22572386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice. AU - Quispe-Salcedo,Angela, AU - Ida-Yonemochi,Hiroko, AU - Nakatomi,Mitsushiro, AU - Ohshima,Hayato, PY - 2012/5/11/entrez PY - 2012/5/11/pubmed PY - 2012/9/5/medline SP - 119 EP - 32 JF - Biomedical research (Tokyo, Japan) JO - Biomed. Res. VL - 33 IS - 2 N2 - Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day- to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity. SN - 1880-313X UR - https://www.unboundmedicine.com/medline/citation/22572386/Expression_patterns_of_nestin_and_dentin_sialoprotein_during_dentinogenesis_in_mice_ L2 - http://japanlinkcenter.org/JST.JSTAGE/biomedres/33.119?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -