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Physical and chemical properties and stability of sodium cefazolin in buffered eye drops determined with HPLC method.
Acta Pol Pharm 2012 Jan-Feb; 69(1):95-105AP

Abstract

The aim of the studies was to analyze the stability of 1% and 5% eye drops containing sodium cefazolin, prepared in citrate buffer of pH 6.11-6.27, which were stored at the temperature of 4 degrees C and 20 degrees C with light protection. The drops were prepared under aseptic conditions by dissolving sodium cefazolin (Biofazolin, IBA Bioton), dry injection form of the drug, in citrate buffer. The viscosity of the drops was increased using polyvinyl alcohol. The drops were preserved with phenylmercuric borate of 0.001% concentration mixed with beta-phenylethyl alcohol of 0.4% concentration in the drops. The concentration of cefazolin was determined at every three days using HPLC method. Besides, the measurements of pH, osmotic pressure and viscosity were performed as well as the organoleptic analysis of the drops (clarity, color, odor). The concentration of cefazolin in 1% drops after the 30-day-storage at the temperature of 4 degrees C, depending on their composition, decreased in the range of 2.17-6.02%. In 5% drops the decrease in cefazolin concentration was similar, i.e., after 30-day-storage at the temperature of 4 degrees C it was 1.62-6.76%. In 1% and 5% drops stored at the temperature of 20 degrees C the stability of the drops determined as the 10% degradation time of cefazolin was in the range of 9-15 days.

Authors+Show Affiliations

Department of Drug Form Technology, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, M. Skłodowskiej-Curie 9, Bydgoszcz, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22574512

Citation

Kodym, Anna, et al. "Physical and Chemical Properties and Stability of Sodium Cefazolin in Buffered Eye Drops Determined With HPLC Method." Acta Poloniae Pharmaceutica, vol. 69, no. 1, 2012, pp. 95-105.
Kodym A, Bilski P, Domańska A, et al. Physical and chemical properties and stability of sodium cefazolin in buffered eye drops determined with HPLC method. Acta Pol Pharm. 2012;69(1):95-105.
Kodym, A., Bilski, P., Domańska, A., Hełminiak, Ł., Jabłońska, M., & Jachymska, A. (2012). Physical and chemical properties and stability of sodium cefazolin in buffered eye drops determined with HPLC method. Acta Poloniae Pharmaceutica, 69(1), pp. 95-105.
Kodym A, et al. Physical and Chemical Properties and Stability of Sodium Cefazolin in Buffered Eye Drops Determined With HPLC Method. Acta Pol Pharm. 2012;69(1):95-105. PubMed PMID: 22574512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical and chemical properties and stability of sodium cefazolin in buffered eye drops determined with HPLC method. AU - Kodym,Anna, AU - Bilski,Piotr, AU - Domańska,Agata, AU - Hełminiak,Łukasz, AU - Jabłońska,Maria, AU - Jachymska,Anna, PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/6/1/medline SP - 95 EP - 105 JF - Acta poloniae pharmaceutica JO - Acta Pol Pharm VL - 69 IS - 1 N2 - The aim of the studies was to analyze the stability of 1% and 5% eye drops containing sodium cefazolin, prepared in citrate buffer of pH 6.11-6.27, which were stored at the temperature of 4 degrees C and 20 degrees C with light protection. The drops were prepared under aseptic conditions by dissolving sodium cefazolin (Biofazolin, IBA Bioton), dry injection form of the drug, in citrate buffer. The viscosity of the drops was increased using polyvinyl alcohol. The drops were preserved with phenylmercuric borate of 0.001% concentration mixed with beta-phenylethyl alcohol of 0.4% concentration in the drops. The concentration of cefazolin was determined at every three days using HPLC method. Besides, the measurements of pH, osmotic pressure and viscosity were performed as well as the organoleptic analysis of the drops (clarity, color, odor). The concentration of cefazolin in 1% drops after the 30-day-storage at the temperature of 4 degrees C, depending on their composition, decreased in the range of 2.17-6.02%. In 5% drops the decrease in cefazolin concentration was similar, i.e., after 30-day-storage at the temperature of 4 degrees C it was 1.62-6.76%. In 1% and 5% drops stored at the temperature of 20 degrees C the stability of the drops determined as the 10% degradation time of cefazolin was in the range of 9-15 days. SN - 0001-6837 UR - https://www.unboundmedicine.com/medline/citation/22574512/Physical_and_chemical_properties_and_stability_of_sodium_cefazolin_in_buffered_eye_drops_determined_with_HPLC_method_ L2 - http://www.ptfarm.pl/pub/File/Acta_Poloniae/2012/1/095.pdf DB - PRIME DP - Unbound Medicine ER -