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[Effects of preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on hypothalamus-pituitary-ovary axis in ovariectomized rats].
Zhen Ci Yan Jiu. 2012 Feb; 37(1):15-9, 45.ZC

Abstract

OBJECTIVE

To observe the effect of preventive-electroacupuncture (PEA) at "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6)on hypothalamus-pituitary-ovary (HPO)activities in ovariectomized (OVX) rats, and to compare the specificity of efficacy of acupoints.

METHODS

Sixty-two female SD rats were randomly divided into normal group (n = 6), sham-operation (sham) group (n = 6), model group (n = 6), PEA-Guanyuan (CV 4, PEA-CV 4) group (n = 11), PEA-Sanyinjiao (SP 6, PEA-SP 6) group (n = 11), complementary (C)-PEA-CV 4 (n = 11) and C-PEA-SP 6 group (n = 11). Climacterium model was duplicated by removing the bilateral ovaries. Before OVX, electroacupuncture (EA, 2 Hz/100 Hz,) was applied to bilateral SP 6, CV 4 for 20 min, once every other day for 15 times. For rats of the C-PEA-CV 4 and C-PEA-SP 6 groups and 12 days after the OVX, PEA was applied to CV 4 and SP 6 again, once every other day for 5 times. For rats of the sham group, a little of fat nearby the ovary was removed. Enzyme-linked immunosorbent assay was used to measure the contents of gonadotropin releasing hormone (GnRH) in the hypothalamus, follicule-stimulating hormone (FSH) and luteinizing hormone (LH) in the hypophysis, and estrogen (E2) in the uterus.

RESULTS

In comparison with the normal group, uterus E2 content was decreased significantly (P < 0. 05), and pituitary FSH and LH contents and hypothalamic GnRH content were increased obviously (P < 0.01) in the model group. Compared with the model group, uterus E2 levels in the PEA-CV 4 and C-PEA-CV 4 groups were increased apparently (P < 0.05), while both pituitary FSH and hypothalamic GnRH levels in the PEA-CV 4, C-PEA-CV 4, PEA-SP 6 and C-PEA-SP 6 groups, and pituitary LH levels in the C-PEA-CV 4 and PEA-SP 6 groups were down-regulated markedly (P < 0.05, P < 0.01), suggesting an improvement of the HPO axis activity following PEA and C-PEA. No significant differences were found between the normal and sham groups in uterus E2, pituitary FSH and LH, and hypothalamic GnRH contents, and between the model and PEA-SP 6 groups, and between the model and C-PEA-SP 6 groups in uterus E2 levels, and between the model and PEA-CV 4 groups, and between the model and C-PEA-SP 6 groups in pituitary LH levels (P > 0.05).

CONCLUSION

Preventive EA of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) can function in up-regulating uterus E2 level, and down-regulating pituitary FSH and LH contents and hypothalamic GnRH level in OVX rats, which may contribute to its efficacy in relieving climacteric syndrome in clinic.

Authors+Show Affiliations

School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.No affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22574563

Citation

Cheng, Kai, and Su-Ling Tian. "[Effects of Preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) On Hypothalamus-pituitary-ovary Axis in Ovariectomized Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 1, 2012, pp. 15-9, 45.
Cheng K, Tian SL. [Effects of preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on hypothalamus-pituitary-ovary axis in ovariectomized rats]. Zhen Ci Yan Jiu. 2012;37(1):15-9, 45.
Cheng, K., & Tian, S. L. (2012). [Effects of preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on hypothalamus-pituitary-ovary axis in ovariectomized rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(1), 15-9, 45.
Cheng K, Tian SL. [Effects of Preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) On Hypothalamus-pituitary-ovary Axis in Ovariectomized Rats]. Zhen Ci Yan Jiu. 2012;37(1):15-9, 45. PubMed PMID: 22574563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of preventive-electroacupuncture of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) on hypothalamus-pituitary-ovary axis in ovariectomized rats]. AU - Cheng,Kai, AU - Tian,Su-Ling, PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/7/18/medline SP - 15-9, 45 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 1 N2 - OBJECTIVE: To observe the effect of preventive-electroacupuncture (PEA) at "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6)on hypothalamus-pituitary-ovary (HPO)activities in ovariectomized (OVX) rats, and to compare the specificity of efficacy of acupoints. METHODS: Sixty-two female SD rats were randomly divided into normal group (n = 6), sham-operation (sham) group (n = 6), model group (n = 6), PEA-Guanyuan (CV 4, PEA-CV 4) group (n = 11), PEA-Sanyinjiao (SP 6, PEA-SP 6) group (n = 11), complementary (C)-PEA-CV 4 (n = 11) and C-PEA-SP 6 group (n = 11). Climacterium model was duplicated by removing the bilateral ovaries. Before OVX, electroacupuncture (EA, 2 Hz/100 Hz,) was applied to bilateral SP 6, CV 4 for 20 min, once every other day for 15 times. For rats of the C-PEA-CV 4 and C-PEA-SP 6 groups and 12 days after the OVX, PEA was applied to CV 4 and SP 6 again, once every other day for 5 times. For rats of the sham group, a little of fat nearby the ovary was removed. Enzyme-linked immunosorbent assay was used to measure the contents of gonadotropin releasing hormone (GnRH) in the hypothalamus, follicule-stimulating hormone (FSH) and luteinizing hormone (LH) in the hypophysis, and estrogen (E2) in the uterus. RESULTS: In comparison with the normal group, uterus E2 content was decreased significantly (P < 0. 05), and pituitary FSH and LH contents and hypothalamic GnRH content were increased obviously (P < 0.01) in the model group. Compared with the model group, uterus E2 levels in the PEA-CV 4 and C-PEA-CV 4 groups were increased apparently (P < 0.05), while both pituitary FSH and hypothalamic GnRH levels in the PEA-CV 4, C-PEA-CV 4, PEA-SP 6 and C-PEA-SP 6 groups, and pituitary LH levels in the C-PEA-CV 4 and PEA-SP 6 groups were down-regulated markedly (P < 0.05, P < 0.01), suggesting an improvement of the HPO axis activity following PEA and C-PEA. No significant differences were found between the normal and sham groups in uterus E2, pituitary FSH and LH, and hypothalamic GnRH contents, and between the model and PEA-SP 6 groups, and between the model and C-PEA-SP 6 groups in uterus E2 levels, and between the model and PEA-CV 4 groups, and between the model and C-PEA-SP 6 groups in pituitary LH levels (P > 0.05). CONCLUSION: Preventive EA of "Guanyuan" (CV 4) and "Sanyinjiao" (SP 6) can function in up-regulating uterus E2 level, and down-regulating pituitary FSH and LH contents and hypothalamic GnRH level in OVX rats, which may contribute to its efficacy in relieving climacteric syndrome in clinic. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/22574563/[Effects_of_preventive_electroacupuncture_of_"Guanyuan"__CV_4__and_"Sanyinjiao"__SP_6__on_hypothalamus_pituitary_ovary_axis_in_ovariectomized_rats]_ DB - PRIME DP - Unbound Medicine ER -