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[Effect of acupuncture on expression of brain-derived neurotrophic factor gene and protein in frontal cortex and hippocampus of depression rats].
Zhen Ci Yan Jiu. 2012 Feb; 37(1):20-4.ZC

Abstract

OBJECTIVE

To observe the influence of acupuncture of "Baihui" (GV 20) to "Yintang" (EX-HN 3) and unilateral "Neiguan" (PC 6) on the expression of brain-derived neurotrophic factor (BDNF) gene and protein in the prefrontal cortex and hippocampus in chronic stress-induced depression rats.

METHODS

Sprague-Dawley rats were randomly divided into four groups (6 rats/group): control, control + acupuncture, model, and model + acupuncture. Depression model was established by giving the rats with repeated abstinence of food and water, illumination at night, forced cold water-swimming, vibration, tail-clamping, constraint and raising alone for 28 days. Acupuncture was applied to "Baihui" (GV 20) to "Yintang" (EX-HN 3) and unilateral "Neiguan"(PC 6) once every other day for 28 days. The expression levels of BDNF mRNA and protein of the frontal cortex and hippocampus tissues were detected by real-time fluorescence quantitative PCR and Western blot methods, separately.

RESULTS

Compared with the control group, the expression levels of BDNF mRNA and protein in the prefrontal cortex and hippocampus were significantly decreased in the model group (P < 0.01, P < 0.05). Whereas compared with the model group, BDNF mRNA and protein expression levels in the prefrontal cortex and hippocampus were significantly upregulated in the model + acupuncture group (P < 0.05, P < 0.01). No significant differences were found between the control and control + acupuncture groups in the expression levels of cortical and hippocampal BDNF mRNA and protein (P > 0.05).

CONCLUSION

Acupuncture can effectively reverse chronic stress-induced down-regulation of BDNF mRNA and protein expression in the frontal cortex and hippocampus in depression rats, which may contribute to its effect in anti-depression by protecting the regeneration of neurons.

Authors+Show Affiliations

Liang J
School of Acu-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
Lu J
No affiliation info available
Cui SF
No affiliation info available
Wang JR
No affiliation info available
Tu Y
No affiliation info available

MeSH

Acupuncture TherapyAnimalsBrain-Derived Neurotrophic FactorCerebral CortexDepressionGene Expression RegulationHippocampusHumansMaleRatsRats, Sprague-Dawley

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22574564

Citation

Liang, Jia, et al. "[Effect of Acupuncture On Expression of Brain-derived Neurotrophic Factor Gene and Protein in Frontal Cortex and Hippocampus of Depression Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 37, no. 1, 2012, pp. 20-4.
Liang J, Lu J, Cui SF, et al. [Effect of acupuncture on expression of brain-derived neurotrophic factor gene and protein in frontal cortex and hippocampus of depression rats]. Zhen Ci Yan Jiu. 2012;37(1):20-4.
Liang, J., Lu, J., Cui, S. F., Wang, J. R., & Tu, Y. (2012). [Effect of acupuncture on expression of brain-derived neurotrophic factor gene and protein in frontal cortex and hippocampus of depression rats]. Zhen Ci Yan Jiu = Acupuncture Research, 37(1), 20-4.
Liang J, et al. [Effect of Acupuncture On Expression of Brain-derived Neurotrophic Factor Gene and Protein in Frontal Cortex and Hippocampus of Depression Rats]. Zhen Ci Yan Jiu. 2012;37(1):20-4. PubMed PMID: 22574564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of acupuncture on expression of brain-derived neurotrophic factor gene and protein in frontal cortex and hippocampus of depression rats]. AU - Liang,Jia, AU - Lu,Jun, AU - Cui,Shan-Fu, AU - Wang,Jun-Ren, AU - Tu,Ya, PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/7/18/medline SP - 20 EP - 4 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 37 IS - 1 N2 - OBJECTIVE: To observe the influence of acupuncture of "Baihui" (GV 20) to "Yintang" (EX-HN 3) and unilateral "Neiguan" (PC 6) on the expression of brain-derived neurotrophic factor (BDNF) gene and protein in the prefrontal cortex and hippocampus in chronic stress-induced depression rats. METHODS: Sprague-Dawley rats were randomly divided into four groups (6 rats/group): control, control + acupuncture, model, and model + acupuncture. Depression model was established by giving the rats with repeated abstinence of food and water, illumination at night, forced cold water-swimming, vibration, tail-clamping, constraint and raising alone for 28 days. Acupuncture was applied to "Baihui" (GV 20) to "Yintang" (EX-HN 3) and unilateral "Neiguan"(PC 6) once every other day for 28 days. The expression levels of BDNF mRNA and protein of the frontal cortex and hippocampus tissues were detected by real-time fluorescence quantitative PCR and Western blot methods, separately. RESULTS: Compared with the control group, the expression levels of BDNF mRNA and protein in the prefrontal cortex and hippocampus were significantly decreased in the model group (P < 0.01, P < 0.05). Whereas compared with the model group, BDNF mRNA and protein expression levels in the prefrontal cortex and hippocampus were significantly upregulated in the model + acupuncture group (P < 0.05, P < 0.01). No significant differences were found between the control and control + acupuncture groups in the expression levels of cortical and hippocampal BDNF mRNA and protein (P > 0.05). CONCLUSION: Acupuncture can effectively reverse chronic stress-induced down-regulation of BDNF mRNA and protein expression in the frontal cortex and hippocampus in depression rats, which may contribute to its effect in anti-depression by protecting the regeneration of neurons. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/22574564/[Effect_of_acupuncture_on_expression_of_brain_derived_neurotrophic_factor_gene_and_protein_in_frontal_cortex_and_hippocampus_of_depression_rats]_ L2 - http://www.diseaseinfosearch.org/result/2199 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓13]

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