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Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice.
J Ethnopharmacol. 2012 Jun 26; 142(1):248-52.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Modified Simiao Decoction (MSD), based on clinical experience, has been used for decades and famous for its efficiency in treating hyperuricemic and gouty diseases.

AIM OF THE STUDY

To investigate the effects of MSD on anti-hyperuricemic and nephroprotective effects are involved in potassium oxonate-induced hyperuricemic mice.

MATERIALS AND METHODS

The effects of MSD were investigated in hyperuricemic mice induced by potassium oxonate. MSD were fed to hyperuricemic mice daily at a dose of 0.45, 0.90, 1.80 g/kg for 10 days, and allopurinol (5mg/kg) was given as a positive control. Serum and urine levels of uric acid and creatinine, and fractional excretion of uric acid (FEUA) were determined by colorimetric method. Its nephroprotective effects were evaluated by determining a panel of oxidative stress markers after the intervention in hyperuricemic mice. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blotting.

RESULTS

MSD could inhibit XOD activities in serum and liver, decrease levels of serum uric acid, serum creatinine and BUN, and increased levels of urine uric acid, urine creatinine, FEUA dose-dependently through down-regulation of URAT1 and up-regulation of OAT1 protein expressions in the renal tissue of hyperuricemic mice. It also effectively reversed oxonate-induced alterations on renal MDA levels and SOD activities in this model.

CONCLUSION

MSD processes uricosuric and nephroprotective actions by regulating renal urate transporters and enhancing antioxidant enzymes activities to improve renal dysfunction in hyperuricemic mice.

Authors+Show Affiliations

The Second People Hospital of Xihu District, Hangzhou 310024, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22575704

Citation

Hua, Jian, et al. "Anti-hyperuricemic and Nephroprotective Effects of Modified Simiao Decoction in Hyperuricemic Mice." Journal of Ethnopharmacology, vol. 142, no. 1, 2012, pp. 248-52.
Hua J, Huang P, Zhu CM, et al. Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice. J Ethnopharmacol. 2012;142(1):248-52.
Hua, J., Huang, P., Zhu, C. M., Yuan, X., & Yu, C. H. (2012). Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice. Journal of Ethnopharmacology, 142(1), 248-52. https://doi.org/10.1016/j.jep.2012.04.052
Hua J, et al. Anti-hyperuricemic and Nephroprotective Effects of Modified Simiao Decoction in Hyperuricemic Mice. J Ethnopharmacol. 2012 Jun 26;142(1):248-52. PubMed PMID: 22575704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-hyperuricemic and nephroprotective effects of Modified Simiao Decoction in hyperuricemic mice. AU - Hua,Jian, AU - Huang,Ping, AU - Zhu,Chong-Mei, AU - Yuan,Xiao, AU - Yu,Chen-Huan, Y1 - 2012/05/08/ PY - 2011/11/21/received PY - 2012/03/02/revised PY - 2012/04/28/accepted PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/10/10/medline SP - 248 EP - 52 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Modified Simiao Decoction (MSD), based on clinical experience, has been used for decades and famous for its efficiency in treating hyperuricemic and gouty diseases. AIM OF THE STUDY: To investigate the effects of MSD on anti-hyperuricemic and nephroprotective effects are involved in potassium oxonate-induced hyperuricemic mice. MATERIALS AND METHODS: The effects of MSD were investigated in hyperuricemic mice induced by potassium oxonate. MSD were fed to hyperuricemic mice daily at a dose of 0.45, 0.90, 1.80 g/kg for 10 days, and allopurinol (5mg/kg) was given as a positive control. Serum and urine levels of uric acid and creatinine, and fractional excretion of uric acid (FEUA) were determined by colorimetric method. Its nephroprotective effects were evaluated by determining a panel of oxidative stress markers after the intervention in hyperuricemic mice. Simultaneously, protein levels of urate transporter 1 (URAT1) and organic anion transporter 1 (OAT1) in the kidney were analyzed by Western blotting. RESULTS: MSD could inhibit XOD activities in serum and liver, decrease levels of serum uric acid, serum creatinine and BUN, and increased levels of urine uric acid, urine creatinine, FEUA dose-dependently through down-regulation of URAT1 and up-regulation of OAT1 protein expressions in the renal tissue of hyperuricemic mice. It also effectively reversed oxonate-induced alterations on renal MDA levels and SOD activities in this model. CONCLUSION: MSD processes uricosuric and nephroprotective actions by regulating renal urate transporters and enhancing antioxidant enzymes activities to improve renal dysfunction in hyperuricemic mice. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22575704/Anti_hyperuricemic_and_nephroprotective_effects_of_Modified_Simiao_Decoction_in_hyperuricemic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00279-6 DB - PRIME DP - Unbound Medicine ER -