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Anticonvulsant and antioxidative activity of hydroalcoholic extract of tuber of Orchis mascula in pentylenetetrazole and maximal electroshock induced seizures in rats.
J Ethnopharmacol. 2012 Jun 26; 142(1):23-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Orchis mascula tuber is used in many polyherbal formulations as a nerve tonic in India.

AIM OF THE STUDY

In the present study, effect of hydroalcholic extract of O. mascula (HEOM) tuber was evaluated against seizures, seizure-induced oxidative stress and cognitive deficit in pentylenetetrazole and maximal electroshock-induced seizures in rats.

MATERIALS AND METHODS

HEOM was administered orally 30 min before induction of seizures by pentylenetetrazole (PTZ; 60 mg/kg, i.p) or maximal electroshock (MES; 70 mA). Elevated plus maze and passive avoidance tests were used to assess the learning and memory. Oxidative stress was studied by estimation of reduced glutathione and lipid peroxidation. Whole brain total cholinesterase activity was also evaluated.

RESULTS

HEOM produced 33.3%, 50% and 66.7% protection in PTZ model and 16.7%, 16.7% and 33.3% at 250, 500 and 1000 mg/kg, respectively, in MES-induced seizures. Pre-treatment with HEOM significantly decreased the retention transfer latency in elevated plus maze test, and an increase in the retention latency in passive avoidance test was observed. Oxidative stress induced by seizures was also attenuated as indicated by significant increase in GSH and decrease in MDA levels in HEOM treated groups. PTZ and MES caused a significant decrease in AChE and BChE activities, which was prevented by HEOM.

CONCLUSIONS

HEOM thus showed protection against seizures, prevented the associated memory impairment probably by modulating cholinergic status and reducing oxidative stress.

Authors+Show Affiliations

Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22575841

Citation

Pahuja, Monika, et al. "Anticonvulsant and Antioxidative Activity of Hydroalcoholic Extract of Tuber of Orchis Mascula in Pentylenetetrazole and Maximal Electroshock Induced Seizures in Rats." Journal of Ethnopharmacology, vol. 142, no. 1, 2012, pp. 23-7.
Pahuja M, Mehla J, Kumar Gupta Y. Anticonvulsant and antioxidative activity of hydroalcoholic extract of tuber of Orchis mascula in pentylenetetrazole and maximal electroshock induced seizures in rats. J Ethnopharmacol. 2012;142(1):23-7.
Pahuja, M., Mehla, J., & Kumar Gupta, Y. (2012). Anticonvulsant and antioxidative activity of hydroalcoholic extract of tuber of Orchis mascula in pentylenetetrazole and maximal electroshock induced seizures in rats. Journal of Ethnopharmacology, 142(1), 23-7. https://doi.org/10.1016/j.jep.2012.04.006
Pahuja M, Mehla J, Kumar Gupta Y. Anticonvulsant and Antioxidative Activity of Hydroalcoholic Extract of Tuber of Orchis Mascula in Pentylenetetrazole and Maximal Electroshock Induced Seizures in Rats. J Ethnopharmacol. 2012 Jun 26;142(1):23-7. PubMed PMID: 22575841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anticonvulsant and antioxidative activity of hydroalcoholic extract of tuber of Orchis mascula in pentylenetetrazole and maximal electroshock induced seizures in rats. AU - Pahuja,Monika, AU - Mehla,Jogender, AU - Kumar Gupta,Yogendra, Y1 - 2012/05/01/ PY - 2011/09/28/received PY - 2012/03/09/revised PY - 2012/04/04/accepted PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/10/10/medline SP - 23 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Orchis mascula tuber is used in many polyherbal formulations as a nerve tonic in India. AIM OF THE STUDY: In the present study, effect of hydroalcholic extract of O. mascula (HEOM) tuber was evaluated against seizures, seizure-induced oxidative stress and cognitive deficit in pentylenetetrazole and maximal electroshock-induced seizures in rats. MATERIALS AND METHODS: HEOM was administered orally 30 min before induction of seizures by pentylenetetrazole (PTZ; 60 mg/kg, i.p) or maximal electroshock (MES; 70 mA). Elevated plus maze and passive avoidance tests were used to assess the learning and memory. Oxidative stress was studied by estimation of reduced glutathione and lipid peroxidation. Whole brain total cholinesterase activity was also evaluated. RESULTS: HEOM produced 33.3%, 50% and 66.7% protection in PTZ model and 16.7%, 16.7% and 33.3% at 250, 500 and 1000 mg/kg, respectively, in MES-induced seizures. Pre-treatment with HEOM significantly decreased the retention transfer latency in elevated plus maze test, and an increase in the retention latency in passive avoidance test was observed. Oxidative stress induced by seizures was also attenuated as indicated by significant increase in GSH and decrease in MDA levels in HEOM treated groups. PTZ and MES caused a significant decrease in AChE and BChE activities, which was prevented by HEOM. CONCLUSIONS: HEOM thus showed protection against seizures, prevented the associated memory impairment probably by modulating cholinergic status and reducing oxidative stress. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22575841/Anticonvulsant_and_antioxidative_activity_of_hydroalcoholic_extract_of_tuber_of_Orchis_mascula_in_pentylenetetrazole_and_maximal_electroshock_induced_seizures_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00230-9 DB - PRIME DP - Unbound Medicine ER -