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Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain.
Genet Mol Res. 2012 Apr 10; 11(2):918-32.GM

Abstract

Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 alleles). The observed heterozygosity ranged from 0.58 to 0.95, while the expected heterozygosity varied between 0.68 and 0.83. The polymorphism information content values ranged from 0.63 to 0.79. The mean polymorphism information content value of 0.70 for the SSR loci provided sufficient discriminating ability to evaluate the genetic diversity among the cultivars. The SSR data allowed unequivocal identification of all the cultivars; a combination of three SSR markers was sufficient to discriminate all 25 olive cultivars. A dendrogram was prepared, using the unweighted pair-group method with arithmetic mean clustering algorithm; it depicted the pattern of relationships between the cultivars. Most of the local cultivars grouped according to their geographic origin. No clear clustering trends were observed when the morphological traits of fruit endocarps or fruit use of cultivars were employed as analysis criteria. We conclude that there is a high level of variability among local olive cultivars from the Extremadura region at both the morphological and molecular levels; these data should be useful for identifying and distinguishing local germplasm.

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Authors+Show Affiliations

Delgado-Martinez FJ
Plant Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain.
Amaya I
No affiliation info available
Sánchez-Sevilla JF
No affiliation info available
Gomez-Jimenez MC
No affiliation info available

MeSH

AllelesBase SequenceDNA, PlantGenetic MarkersHeterozygoteMicrosatellite RepeatsOleaPolymorphism, GeneticSpain

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22576919

Citation

Delgado-Martinez, F J., et al. "Microsatellite Marker-based Identification and Genetic Relationships of Olive Cultivars From the Extremadura Region of Spain." Genetics and Molecular Research : GMR, vol. 11, no. 2, 2012, pp. 918-32.
Delgado-Martinez FJ, Amaya I, Sánchez-Sevilla JF, et al. Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain. Genet Mol Res. 2012;11(2):918-32.
Delgado-Martinez, F. J., Amaya, I., Sánchez-Sevilla, J. F., & Gomez-Jimenez, M. C. (2012). Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain. Genetics and Molecular Research : GMR, 11(2), 918-32. https://doi.org/10.4238/2012.April.10.7
Delgado-Martinez FJ, et al. Microsatellite Marker-based Identification and Genetic Relationships of Olive Cultivars From the Extremadura Region of Spain. Genet Mol Res. 2012 Apr 10;11(2):918-32. PubMed PMID: 22576919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain. AU - Delgado-Martinez,F J, AU - Amaya,I, AU - Sánchez-Sevilla,J F, AU - Gomez-Jimenez,M C, Y1 - 2012/04/10/ PY - 2012/5/12/entrez PY - 2012/5/12/pubmed PY - 2012/9/11/medline SP - 918 EP - 32 JF - Genetics and molecular research : GMR JO - Genet Mol Res VL - 11 IS - 2 N2 - Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 alleles). The observed heterozygosity ranged from 0.58 to 0.95, while the expected heterozygosity varied between 0.68 and 0.83. The polymorphism information content values ranged from 0.63 to 0.79. The mean polymorphism information content value of 0.70 for the SSR loci provided sufficient discriminating ability to evaluate the genetic diversity among the cultivars. The SSR data allowed unequivocal identification of all the cultivars; a combination of three SSR markers was sufficient to discriminate all 25 olive cultivars. A dendrogram was prepared, using the unweighted pair-group method with arithmetic mean clustering algorithm; it depicted the pattern of relationships between the cultivars. Most of the local cultivars grouped according to their geographic origin. No clear clustering trends were observed when the morphological traits of fruit endocarps or fruit use of cultivars were employed as analysis criteria. We conclude that there is a high level of variability among local olive cultivars from the Extremadura region at both the morphological and molecular levels; these data should be useful for identifying and distinguishing local germplasm. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/22576919/Microsatellite_marker_based_identification_and_genetic_relationships_of_olive_cultivars_from_the_Extremadura_region_of_Spain_ L2 - http://www.geneticsmr.com/articles/1583 DB - PRIME DP - Unbound Medicine ER -