Tags

Type your tag names separated by a space and hit enter

Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo.
Br J Dermatol. 2012 Oct; 167(4):787-93.BJ

Abstract

BACKGROUND

The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied.

OBJECTIVES

We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin.

METHODS

Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63.9 and 32.6 years) were sequentially exposed to 0.5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme-linked immunosorbent assay.

RESULTS

Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*-value and capacitance, assessed by the respective Δ-values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)-1α levels, whereas the application of Tol/Tol induced an increase in IL-1α. Furthermore, decreased IL-1 receptor antagonist (IL-1RA) levels and a lower IL-1RA/IL-1α ratio were found following repeated exposure to the irritants.

CONCLUSIONS

Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo.

Authors+Show Affiliations

Department of Dermatology, University of Lübeck, Germany. irena.angelova-fischer@uk-sh.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22578221

Citation

Angelova-Fischer, I, et al. "Tandem Repeated Irritation in Aged Skin Induces Distinct Barrier Perturbation and Cytokine Profile in Vivo." The British Journal of Dermatology, vol. 167, no. 4, 2012, pp. 787-93.
Angelova-Fischer I, Becker V, Fischer TW, et al. Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo. Br J Dermatol. 2012;167(4):787-93.
Angelova-Fischer, I., Becker, V., Fischer, T. W., Zillikens, D., Wigger-Alberti, W., & Kezic, S. (2012). Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo. The British Journal of Dermatology, 167(4), 787-93. https://doi.org/10.1111/j.1365-2133.2012.11049.x
Angelova-Fischer I, et al. Tandem Repeated Irritation in Aged Skin Induces Distinct Barrier Perturbation and Cytokine Profile in Vivo. Br J Dermatol. 2012;167(4):787-93. PubMed PMID: 22578221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tandem repeated irritation in aged skin induces distinct barrier perturbation and cytokine profile in vivo. AU - Angelova-Fischer,I, AU - Becker,V, AU - Fischer,T W, AU - Zillikens,D, AU - Wigger-Alberti,W, AU - Kezic,S, Y1 - 2012/08/27/ PY - 2012/5/15/entrez PY - 2012/5/15/pubmed PY - 2013/4/5/medline SP - 787 EP - 93 JF - The British journal of dermatology JO - Br J Dermatol VL - 167 IS - 4 N2 - BACKGROUND: The barrier perturbation pattern and molecular markers of inflammation upon tandem repeated irritation in chronologically aged skin have not been previously studied. OBJECTIVES: We aimed to investigate the barrier impairment kinetic and in vivo cytokine profile following sequential irritation with sodium lauryl sulfate (SLS) and undiluted toluene (Tol) in aged compared with young skin. METHODS: Four fields on the volar forearm of healthy aged and young volunteers (median age, respectively, 63.9 and 32.6 years) were sequentially exposed to 0.5% SLS and undiluted toluene in a controlled tandem repeated irritation test; an adjacent nontreated field served as control. The permeability barrier function was monitored by repeated measurements of transepidermal water loss (TEWL), capacitance and erythema every 24 h up to 96 h. The stratum corneum cytokines were harvested by sequential tape stripping and quantified by multiplex bead array and enzyme-linked immunosorbent assay. RESULTS: Compared with young skin, aged skin was characterized by delayed and/or less pronounced alterations in the visual irritation score, TEWL, chromametry a*-value and capacitance, assessed by the respective Δ-values for each parameter and monitoring time point. In both groups, exposure to SLS/SLS, SLS/Tol and Tol/SLS resulted in decreased interleukin (IL)-1α levels, whereas the application of Tol/Tol induced an increase in IL-1α. Furthermore, decreased IL-1 receptor antagonist (IL-1RA) levels and a lower IL-1RA/IL-1α ratio were found following repeated exposure to the irritants. CONCLUSIONS: Our results provide evidence for selective alterations in the cytokine profile and distinct barrier impairment kinetic following tandem repeated irritation with SLS and Tol in aged compared with young skin in vivo. SN - 1365-2133 UR - https://www.unboundmedicine.com/medline/citation/22578221/Tandem_repeated_irritation_in_aged_skin_induces_distinct_barrier_perturbation_and_cytokine_profile_in_vivo_ L2 - https://doi.org/10.1111/j.1365-2133.2012.11049.x DB - PRIME DP - Unbound Medicine ER -