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Association between PCR ribotypes and antimicrobial susceptibility among Clostridium difficile isolates from healthcare-associated infections in South Korea.
Int J Antimicrob Agents. 2012 Jul; 40(1):24-9.IJ

Abstract

In this study, the association between antimicrobial susceptibility, PCR ribotype and presence of the ermB gene in clinical isolates of Clostridium difficile was investigated. PCR ribotyping and ermB gene PCR were performed on 131 C. difficile isolates. The susceptibility of these isolates to metronidazole, vancomycin, piperacillin/tazobactam (TZP), clindamycin, moxifloxacin and rifaximin was also determined. Use of antibiotics within the previous 2 months was documented. Resistance rates to clindamycin, moxifloxacin and rifaximin were 67.9%, 62.6% and 19.1%, respectively. No metronidazole, vancomycin or TZP resistance was detected. Previous exposure to moxifloxacin was significantly correlated with resistance to this antibiotic, but prior use of clindamycin was not significantly correlated with clindamycin resistance. Sixty-four strains (48.9%) carried the ermB gene, of which all but one (98.5%) were resistant to clindamycin. The clindamycin resistance rates of the common PCR ribotypes (018, 017 and 001) were 91.4%, 100% and 84.2%, respectively, and their moxifloxacin resistance rates were 91.4%, 95.0% and 78.9%, respectively. Resistance rates to rifaximin were 5.7% and 95.0% in ribotype 018 and 017 strains, whilst none of the 001 strains were resistant to rifaximin. In conclusion, the common ribotypes 018, 017 and 001 of C. difficile have high rates of resistance to clindamycin and moxifloxacin, but differ greatly in the frequency of rifaximin resistance.

Authors+Show Affiliations

Department of Internal Medicine, Hanyang University College of Medicine, Seoul, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22578765

Citation

Kim, Jieun, et al. "Association Between PCR Ribotypes and Antimicrobial Susceptibility Among Clostridium Difficile Isolates From Healthcare-associated Infections in South Korea." International Journal of Antimicrobial Agents, vol. 40, no. 1, 2012, pp. 24-9.
Kim J, Kang JO, Pai H, et al. Association between PCR ribotypes and antimicrobial susceptibility among Clostridium difficile isolates from healthcare-associated infections in South Korea. Int J Antimicrob Agents. 2012;40(1):24-9.
Kim, J., Kang, J. O., Pai, H., & Choi, T. Y. (2012). Association between PCR ribotypes and antimicrobial susceptibility among Clostridium difficile isolates from healthcare-associated infections in South Korea. International Journal of Antimicrobial Agents, 40(1), 24-9. https://doi.org/10.1016/j.ijantimicag.2012.03.015
Kim J, et al. Association Between PCR Ribotypes and Antimicrobial Susceptibility Among Clostridium Difficile Isolates From Healthcare-associated Infections in South Korea. Int J Antimicrob Agents. 2012;40(1):24-9. PubMed PMID: 22578765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between PCR ribotypes and antimicrobial susceptibility among Clostridium difficile isolates from healthcare-associated infections in South Korea. AU - Kim,Jieun, AU - Kang,Jung Oak, AU - Pai,Hyunjoo, AU - Choi,Tae Yeal, Y1 - 2012/05/11/ PY - 2012/03/02/received PY - 2012/03/20/revised PY - 2012/03/22/accepted PY - 2012/5/15/entrez PY - 2012/5/15/pubmed PY - 2012/9/29/medline SP - 24 EP - 9 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 40 IS - 1 N2 - In this study, the association between antimicrobial susceptibility, PCR ribotype and presence of the ermB gene in clinical isolates of Clostridium difficile was investigated. PCR ribotyping and ermB gene PCR were performed on 131 C. difficile isolates. The susceptibility of these isolates to metronidazole, vancomycin, piperacillin/tazobactam (TZP), clindamycin, moxifloxacin and rifaximin was also determined. Use of antibiotics within the previous 2 months was documented. Resistance rates to clindamycin, moxifloxacin and rifaximin were 67.9%, 62.6% and 19.1%, respectively. No metronidazole, vancomycin or TZP resistance was detected. Previous exposure to moxifloxacin was significantly correlated with resistance to this antibiotic, but prior use of clindamycin was not significantly correlated with clindamycin resistance. Sixty-four strains (48.9%) carried the ermB gene, of which all but one (98.5%) were resistant to clindamycin. The clindamycin resistance rates of the common PCR ribotypes (018, 017 and 001) were 91.4%, 100% and 84.2%, respectively, and their moxifloxacin resistance rates were 91.4%, 95.0% and 78.9%, respectively. Resistance rates to rifaximin were 5.7% and 95.0% in ribotype 018 and 017 strains, whilst none of the 001 strains were resistant to rifaximin. In conclusion, the common ribotypes 018, 017 and 001 of C. difficile have high rates of resistance to clindamycin and moxifloxacin, but differ greatly in the frequency of rifaximin resistance. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/22578765/Association_between_PCR_ribotypes_and_antimicrobial_susceptibility_among_Clostridium_difficile_isolates_from_healthcare_associated_infections_in_South_Korea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(12)00142-2 DB - PRIME DP - Unbound Medicine ER -