Tags

Type your tag names separated by a space and hit enter

Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide.
Vet Immunol Immunopathol 2012; 147(3-4):211-22VI

Abstract

The Toll-like receptor (TLR)4 is critical for the recognition of Gram-negative bacterial lipopolysaccharide (LPS) but in porcine peripheral blood mononuclear cells (PBMCs) it may cooperate with other TLRs and lead to the production of inflammatory cytokines. Therefore, we analyzed TLR1-10 mRNA expression in porcine PBMCs stimulated with LPS over time (1-48 h) by using quantitative real-time PCR and cytokine proteins level by ELISA in culture supernatant. TLR1-10 mRNA was detectable in porcine PBMCs. When compared with the control (non-stimulated), TLR1 mRNA were increased (p<0.05) at 3 h after challenge with 1 μg/ml LPS, whereas TLR1 and TLR2 mRNA were increased (p<0.01) at 6 h after challenge with 10 μg/ml LPS. TLR4 increased (p<0.001) at 3h after challenge with LPS and remained constant. TLR5 and TLR6 mRNA increased (p<0.05) at 9 h and 1 h after of LPS stimulation, respectively. The mRNA of CD14 and MD2 were increased (p<0.001) at 1h after LPS stimulation. Additionally, at most of the time analyzed, the mRNA expression increased with the dose of LPS. The LPS concentration had influence (p<0.05) on all the TLRs expression except TLR10; whereas time had effect (p<0.05) on all TLRs expression except TLR2, 3, 6 and 10. When compared to the control, the cytokines IL1b, IL8 and TNFα proteins were increased (p<0.001) immediately at 1 h after LPS stimulation and remained constant till 48 h. IL12b was increased (p<0.001) 12 h after challenge with 10 μg/ml of LPS. Although IL8 level was the highest, the higher (p<0.05) expression of all these inflammatory cytokines indicate that upon interacting with TLRs, LPS exerted inflammatory response in PBMCs through the production of Th1 type cytokines. The production of cytokines was influenced (p<0.001) by both the dose of LPS and the stimulation time. Hence, the porcine PBMCs are likely able to express all members of TLRs.

Authors+Show Affiliations

Institute of Animal Science, University of Bonn, Endenicher Allee 15, 53115 Bonn, Germany. judd@itw.uni-bonn.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22578850

Citation

Uddin, Muhammad Jasim, et al. "Expression Dynamics of Toll-like Receptors mRNA and Cytokines in Porcine Peripheral Blood Mononuclear Cells Stimulated By Bacterial Lipopolysaccharide." Veterinary Immunology and Immunopathology, vol. 147, no. 3-4, 2012, pp. 211-22.
Uddin MJ, Nuro-Gyina PK, Islam MA, et al. Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide. Vet Immunol Immunopathol. 2012;147(3-4):211-22.
Uddin, M. J., Nuro-Gyina, P. K., Islam, M. A., Tesfaye, D., Tholen, E., Looft, C., ... Cinar, M. U. (2012). Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide. Veterinary Immunology and Immunopathology, 147(3-4), pp. 211-22. doi:10.1016/j.vetimm.2012.04.020.
Uddin MJ, et al. Expression Dynamics of Toll-like Receptors mRNA and Cytokines in Porcine Peripheral Blood Mononuclear Cells Stimulated By Bacterial Lipopolysaccharide. Vet Immunol Immunopathol. 2012 Jun 30;147(3-4):211-22. PubMed PMID: 22578850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide. AU - Uddin,Muhammad Jasim, AU - Nuro-Gyina,Patrick Kwadwo, AU - Islam,Mohammad Ariful, AU - Tesfaye,Dawit, AU - Tholen,Ernst, AU - Looft,Christian, AU - Schellander,Karl, AU - Cinar,Mehmet Ulas, Y1 - 2012/04/26/ PY - 2011/11/02/received PY - 2012/04/07/revised PY - 2012/04/20/accepted PY - 2012/5/15/entrez PY - 2012/5/15/pubmed PY - 2012/9/27/medline SP - 211 EP - 22 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 147 IS - 3-4 N2 - The Toll-like receptor (TLR)4 is critical for the recognition of Gram-negative bacterial lipopolysaccharide (LPS) but in porcine peripheral blood mononuclear cells (PBMCs) it may cooperate with other TLRs and lead to the production of inflammatory cytokines. Therefore, we analyzed TLR1-10 mRNA expression in porcine PBMCs stimulated with LPS over time (1-48 h) by using quantitative real-time PCR and cytokine proteins level by ELISA in culture supernatant. TLR1-10 mRNA was detectable in porcine PBMCs. When compared with the control (non-stimulated), TLR1 mRNA were increased (p<0.05) at 3 h after challenge with 1 μg/ml LPS, whereas TLR1 and TLR2 mRNA were increased (p<0.01) at 6 h after challenge with 10 μg/ml LPS. TLR4 increased (p<0.001) at 3h after challenge with LPS and remained constant. TLR5 and TLR6 mRNA increased (p<0.05) at 9 h and 1 h after of LPS stimulation, respectively. The mRNA of CD14 and MD2 were increased (p<0.001) at 1h after LPS stimulation. Additionally, at most of the time analyzed, the mRNA expression increased with the dose of LPS. The LPS concentration had influence (p<0.05) on all the TLRs expression except TLR10; whereas time had effect (p<0.05) on all TLRs expression except TLR2, 3, 6 and 10. When compared to the control, the cytokines IL1b, IL8 and TNFα proteins were increased (p<0.001) immediately at 1 h after LPS stimulation and remained constant till 48 h. IL12b was increased (p<0.001) 12 h after challenge with 10 μg/ml of LPS. Although IL8 level was the highest, the higher (p<0.05) expression of all these inflammatory cytokines indicate that upon interacting with TLRs, LPS exerted inflammatory response in PBMCs through the production of Th1 type cytokines. The production of cytokines was influenced (p<0.001) by both the dose of LPS and the stimulation time. Hence, the porcine PBMCs are likely able to express all members of TLRs. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/22578850/Expression_dynamics_of_Toll_like_receptors_mRNA_and_cytokines_in_porcine_peripheral_blood_mononuclear_cells_stimulated_by_bacterial_lipopolysaccharide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(12)00131-6 DB - PRIME DP - Unbound Medicine ER -