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Oxidative stress status and DNA damage in saliva of human subjects with oral lichen planus and oral squamous cell carcinoma.
J Oral Pathol Med. 2012 Nov; 41(10):736-40.JO

Abstract

BACKGROUND

The aim of this study was to evaluate oxidative stress status in the saliva of patients with oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC).

METHODS

Thirty-two patients with OLP, 26 patients with OSCC, and 30 non-involved subjects were enrolled in this study. The study was conducted at the Cancer Department, Clinic of Oral Medicine, Tehran University of Medical Sciences. The unstimulated whole saliva malondialdehyde (MDA), as an indicator of lipid peroxidation, the total antioxidant capacity (TAC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were assayed by thiobarbituric acid, ferric reducing antioxidant potential (FRAP), and ELISA method, respectively. The TAC/MDA ratio was used as an index of oxidative stress status. Data were analyzed by ANOVA followed by the Tukey's post hoc test.

RESULTS AND CONCLUSION

There were no significant differences in saliva TAC and MDA levels between OLP and control, and also between OLP and OSCC patients. MDA and 8-OHdG were significantly higher but TAC was lower in OSCC patients than control. TAC/MDA ratio was significantly lower in patients with OSCC than both OLP patients and control. TAC/MDA ratio was significantly lower but 8-OHdG was higher in patients with OLP compared to control. This suggests that patients with OLP and OSCC are more susceptible to an imbalance of antioxidant-oxidative stress status.

Authors+Show Affiliations

Department of Oral Medicine, Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran. aghahose@sina.tums.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22582895

Citation

Agha-Hosseini, Farzaneh, et al. "Oxidative Stress Status and DNA Damage in Saliva of Human Subjects With Oral Lichen Planus and Oral Squamous Cell Carcinoma." Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, vol. 41, no. 10, 2012, pp. 736-40.
Agha-Hosseini F, Mirzaii-Dizgah I, Farmanbar N, et al. Oxidative stress status and DNA damage in saliva of human subjects with oral lichen planus and oral squamous cell carcinoma. J Oral Pathol Med. 2012;41(10):736-40.
Agha-Hosseini, F., Mirzaii-Dizgah, I., Farmanbar, N., & Abdollahi, M. (2012). Oxidative stress status and DNA damage in saliva of human subjects with oral lichen planus and oral squamous cell carcinoma. Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 41(10), 736-40. https://doi.org/10.1111/j.1600-0714.2012.01172.x
Agha-Hosseini F, et al. Oxidative Stress Status and DNA Damage in Saliva of Human Subjects With Oral Lichen Planus and Oral Squamous Cell Carcinoma. J Oral Pathol Med. 2012;41(10):736-40. PubMed PMID: 22582895.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidative stress status and DNA damage in saliva of human subjects with oral lichen planus and oral squamous cell carcinoma. AU - Agha-Hosseini,Farzaneh, AU - Mirzaii-Dizgah,Iraj, AU - Farmanbar,Niloofar, AU - Abdollahi,Mohammad, Y1 - 2012/05/15/ PY - 2012/5/16/entrez PY - 2012/5/16/pubmed PY - 2013/5/31/medline SP - 736 EP - 40 JF - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology JO - J Oral Pathol Med VL - 41 IS - 10 N2 - BACKGROUND: The aim of this study was to evaluate oxidative stress status in the saliva of patients with oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC). METHODS: Thirty-two patients with OLP, 26 patients with OSCC, and 30 non-involved subjects were enrolled in this study. The study was conducted at the Cancer Department, Clinic of Oral Medicine, Tehran University of Medical Sciences. The unstimulated whole saliva malondialdehyde (MDA), as an indicator of lipid peroxidation, the total antioxidant capacity (TAC), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were assayed by thiobarbituric acid, ferric reducing antioxidant potential (FRAP), and ELISA method, respectively. The TAC/MDA ratio was used as an index of oxidative stress status. Data were analyzed by ANOVA followed by the Tukey's post hoc test. RESULTS AND CONCLUSION: There were no significant differences in saliva TAC and MDA levels between OLP and control, and also between OLP and OSCC patients. MDA and 8-OHdG were significantly higher but TAC was lower in OSCC patients than control. TAC/MDA ratio was significantly lower in patients with OSCC than both OLP patients and control. TAC/MDA ratio was significantly lower but 8-OHdG was higher in patients with OLP compared to control. This suggests that patients with OLP and OSCC are more susceptible to an imbalance of antioxidant-oxidative stress status. SN - 1600-0714 UR - https://www.unboundmedicine.com/medline/citation/22582895/Oxidative_stress_status_and_DNA_damage_in_saliva_of_human_subjects_with_oral_lichen_planus_and_oral_squamous_cell_carcinoma_ L2 - https://doi.org/10.1111/j.1600-0714.2012.01172.x DB - PRIME DP - Unbound Medicine ER -