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CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection.

Abstract

Antigen-specific antibodies are well characterized after vaccination with pandemic H1N1 or seasonal influenza vaccines. However, knowledge on cellular immunity toward pandemic H1N1 after vaccination and infection and cross-reactivities toward seasonal antigens is limited. Nineteen individuals were vaccinated with the pandemic H1N1 vaccine. Among those, ten had been prevaccinated against seasonal influenza. CD4(+) T cells specific for pandemic H1N1 and for seasonal vaccine, and antibodies were monitored using flow cytometry and ELISA/neutralization assays, respectively. In addition, seven patients with acute pandemic influenza infection were analyzed. Pandemic H1N1 vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific CD4(+) T cells that was more pronounced in individuals not prevaccinated with seasonal influenza (p = 0.01). T-cell levels toward seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26). Likewise, prevaccination with seasonal influenza induced a less pronounced increase in specific antibodies. Influenza-specific T cells in vaccinees had a Th1 phenotype mainly coexpressing IFN-γ and IL-2, whereas patients with active pandemic influenza showed a shift toward cells predominantly expressing IFN-γ. In conclusion, T cells toward seasonal influenza antigens cross-react with pandemic H1N1 antigens and affect induction of specific T cells after pandemic influenza vaccination. In addition, the cytokine patterns of specific T cells during acute H1N1 infection and after vaccination differ, and the predominantly dual-positive cytokine profile of vaccine-induced T cells suggests sufficient functionality to confer successful virus control.

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  • Authors+Show Affiliations

    ,

    Department of Transplant and Infection Immunology, Saarland University, Homburg, Germany.

    , , , , ,

    Source

    European journal of immunology 42:7 2012 Jul pg 1755-66

    MeSH

    Adult
    Antibodies, Viral
    CD4-Positive T-Lymphocytes
    Cross Reactions
    Enzyme-Linked Immunosorbent Assay
    Flow Cytometry
    Humans
    Immunity, Cellular
    Influenza A Virus, H1N1 Subtype
    Influenza Vaccines
    Influenza, Human
    Interferon-gamma
    Interleukin-2
    Middle Aged
    Neutralization Tests
    Pandemics
    Statistics, Nonparametric

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22585549

    Citation

    Schmidt, Tina, et al. "CD4+ T-cell Immunity After Pandemic Influenza Vaccination Cross-reacts With Seasonal Antigens and Functionally Differs From Active Influenza Infection." European Journal of Immunology, vol. 42, no. 7, 2012, pp. 1755-66.
    Schmidt T, Dirks J, Enders M, et al. CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection. Eur J Immunol. 2012;42(7):1755-66.
    Schmidt, T., Dirks, J., Enders, M., Gärtner, B. C., Uhlmann-Schiffler, H., Sester, U., & Sester, M. (2012). CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection. European Journal of Immunology, 42(7), pp. 1755-66. doi:10.1002/eji.201242393.
    Schmidt T, et al. CD4+ T-cell Immunity After Pandemic Influenza Vaccination Cross-reacts With Seasonal Antigens and Functionally Differs From Active Influenza Infection. Eur J Immunol. 2012;42(7):1755-66. PubMed PMID: 22585549.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - CD4+ T-cell immunity after pandemic influenza vaccination cross-reacts with seasonal antigens and functionally differs from active influenza infection. AU - Schmidt,Tina, AU - Dirks,Jan, AU - Enders,Martin, AU - Gärtner,Barbara C, AU - Uhlmann-Schiffler,Heike, AU - Sester,Urban, AU - Sester,Martina, PY - 2012/5/16/entrez PY - 2012/5/16/pubmed PY - 2012/12/10/medline SP - 1755 EP - 66 JF - European journal of immunology JO - Eur. J. Immunol. VL - 42 IS - 7 N2 - Antigen-specific antibodies are well characterized after vaccination with pandemic H1N1 or seasonal influenza vaccines. However, knowledge on cellular immunity toward pandemic H1N1 after vaccination and infection and cross-reactivities toward seasonal antigens is limited. Nineteen individuals were vaccinated with the pandemic H1N1 vaccine. Among those, ten had been prevaccinated against seasonal influenza. CD4(+) T cells specific for pandemic H1N1 and for seasonal vaccine, and antibodies were monitored using flow cytometry and ELISA/neutralization assays, respectively. In addition, seven patients with acute pandemic influenza infection were analyzed. Pandemic H1N1 vaccination induced a strong 4.63-fold (IQR 4.16) increase in antigen-specific CD4(+) T cells that was more pronounced in individuals not prevaccinated with seasonal influenza (p = 0.01). T-cell levels toward seasonal vaccine concomitantly rose by 2.71-fold (IQR 2.26). Likewise, prevaccination with seasonal influenza induced a less pronounced increase in specific antibodies. Influenza-specific T cells in vaccinees had a Th1 phenotype mainly coexpressing IFN-γ and IL-2, whereas patients with active pandemic influenza showed a shift toward cells predominantly expressing IFN-γ. In conclusion, T cells toward seasonal influenza antigens cross-react with pandemic H1N1 antigens and affect induction of specific T cells after pandemic influenza vaccination. In addition, the cytokine patterns of specific T cells during acute H1N1 infection and after vaccination differ, and the predominantly dual-positive cytokine profile of vaccine-induced T cells suggests sufficient functionality to confer successful virus control. SN - 1521-4141 UR - https://www.unboundmedicine.com/medline/citation/22585549/CD4+_T_cell_immunity_after_pandemic_influenza_vaccination_cross_reacts_with_seasonal_antigens_and_functionally_differs_from_active_influenza_infection_ L2 - https://doi.org/10.1002/eji.201242393 DB - PRIME DP - Unbound Medicine ER -