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Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes.
Hum Reprod. 2012 Jul; 27(7):2160-8.HR

Abstract

BACKGROUND

Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes.

METHODS

Unfertilized MII oocytes following ICSI failure were cryopreserved either by slow freezing or by the Cryotip method for vitrification. After thawing, total RNA was extracted and analyzed using Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays. The gene expression profiles and associated biological pathways in slowly frozen/thawed and vitrified MII oocytes were determined and compared with those of non-cryopreserved MII oocytes used as controls.

RESULTS

Both cryopreservation procedures negatively affected the gene expression profile of human MII oocytes in comparison with controls. However, slowly frozen and vitrified MI oocytes displayed specific gene expression signatures. Slow freezing was associated with down-regulation of genes involved in chromosomal structure maintenance (KIF2C and KIF3A) and cell cycle regulation (CHEK2 and CDKN1B) that may lead to a reduction in the oocyte developmental competence. In vitrified oocytes, many genes of the ubiquitination pathway were down-regulated, including members of the ubiquitin-specific peptidase family and subunits of the 26S proteasome. Such inhibition of the degradation machinery might stabilize the maternal protein content that is necessary for oocyte developmental competence.

CONCLUSIONS

The low pregnancy rates commonly observed when using human MII oocytes after slow freezing-thawing may be explained by the alterations of the oocyte gene expression profile.

Authors+Show Affiliations

CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, Montpellier F-34295, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22587994

Citation

Monzo, C, et al. "Slow Freezing and Vitrification Differentially Modify the Gene Expression Profile of Human Metaphase II Oocytes." Human Reproduction (Oxford, England), vol. 27, no. 7, 2012, pp. 2160-8.
Monzo C, Haouzi D, Roman K, et al. Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes. Hum Reprod. 2012;27(7):2160-8.
Monzo, C., Haouzi, D., Roman, K., Assou, S., Dechaud, H., & Hamamah, S. (2012). Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes. Human Reproduction (Oxford, England), 27(7), 2160-8. https://doi.org/10.1093/humrep/des153
Monzo C, et al. Slow Freezing and Vitrification Differentially Modify the Gene Expression Profile of Human Metaphase II Oocytes. Hum Reprod. 2012;27(7):2160-8. PubMed PMID: 22587994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Slow freezing and vitrification differentially modify the gene expression profile of human metaphase II oocytes. AU - Monzo,C, AU - Haouzi,D, AU - Roman,K, AU - Assou,S, AU - Dechaud,H, AU - Hamamah,S, Y1 - 2012/05/15/ PY - 2012/5/17/entrez PY - 2012/5/17/pubmed PY - 2012/11/14/medline SP - 2160 EP - 8 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 27 IS - 7 N2 - BACKGROUND: Cryopreservation is now considered as an efficient way to store human oocytes to preserve fertility. However, little is known about the effects of this technology on oocyte gene expression. The aim of this study was to examine the effect of the two cryopreservation procedures, slow freezing and vitrification, on the gene expression profile of human metaphase II (MII) oocytes. METHODS: Unfertilized MII oocytes following ICSI failure were cryopreserved either by slow freezing or by the Cryotip method for vitrification. After thawing, total RNA was extracted and analyzed using Affymetrix Human Genome U133 Plus 2.0 GeneChip arrays. The gene expression profiles and associated biological pathways in slowly frozen/thawed and vitrified MII oocytes were determined and compared with those of non-cryopreserved MII oocytes used as controls. RESULTS: Both cryopreservation procedures negatively affected the gene expression profile of human MII oocytes in comparison with controls. However, slowly frozen and vitrified MI oocytes displayed specific gene expression signatures. Slow freezing was associated with down-regulation of genes involved in chromosomal structure maintenance (KIF2C and KIF3A) and cell cycle regulation (CHEK2 and CDKN1B) that may lead to a reduction in the oocyte developmental competence. In vitrified oocytes, many genes of the ubiquitination pathway were down-regulated, including members of the ubiquitin-specific peptidase family and subunits of the 26S proteasome. Such inhibition of the degradation machinery might stabilize the maternal protein content that is necessary for oocyte developmental competence. CONCLUSIONS: The low pregnancy rates commonly observed when using human MII oocytes after slow freezing-thawing may be explained by the alterations of the oocyte gene expression profile. SN - 1460-2350 UR - https://www.unboundmedicine.com/medline/citation/22587994/Slow_freezing_and_vitrification_differentially_modify_the_gene_expression_profile_of_human_metaphase_II_oocytes_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/des153 DB - PRIME DP - Unbound Medicine ER -