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Co-circulation of four human coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004.
Viruses. 2012 04; 4(4):637-53.V

Abstract

Acute respiratory illnesses (ARIs) with unconfirmed infectious aetiologies peak at different times of the year. Molecular diagnostic assays reduce the number of unconfirmed ARIs compared to serology- or culture-based techniques. Screening of 888 inpatient and outpatient respiratory specimens spanning late autumn through to early spring, 2004, identified the presence of a human coronavirus (HCoV) on 74 occasions (8.3% of all specimens and 26.3% of all respiratory virus detections). Prevalence peaked in August (late winter in the southern hemisphere) when they were detected in 21.9% of specimens tested. HCoV-HKU1 and HCoV-OC43 comprised 82.4% of all HCoVs detected. Positive specimens were used to develop novel reverse transcriptase real-time PCRs (RT-rtPCRs) for HCoV detection. An objective clinical severity score was assigned to each positive HCoV patient. Severity scores were similar to those from a random selection of young children who were positive for respiratory syncytial virus at a different time but from the same specimen population. During the cooler months of 2004, sensitive and specific RT-rtPCRs identified the concurrent circulation of all four HCoVs, a quarter of which co-occurred with another virus and most of which were from children under the age of two years.

Authors+Show Affiliations

Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Sir Albert Sakzewski Virus Research Centre, Children's Health Service, University of Queensland, Herston 4029, Australia. ian.mackay@uq.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22590689

Citation

Mackay, Ian M., et al. "Co-circulation of Four Human Coronaviruses (HCoVs) in Queensland Children With Acute Respiratory Tract Illnesses in 2004." Viruses, vol. 4, no. 4, 2012, pp. 637-53.
Mackay IM, Arden KE, Speicher DJ, et al. Co-circulation of four human coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004. Viruses. 2012;4(4):637-53.
Mackay, I. M., Arden, K. E., Speicher, D. J., O'Neil, N. T., McErlean, P. K., Greer, R. M., Nissen, M. D., & Sloots, T. P. (2012). Co-circulation of four human coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004. Viruses, 4(4), 637-53. https://doi.org/10.3390/v4040637
Mackay IM, et al. Co-circulation of Four Human Coronaviruses (HCoVs) in Queensland Children With Acute Respiratory Tract Illnesses in 2004. Viruses. 2012;4(4):637-53. PubMed PMID: 22590689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-circulation of four human coronaviruses (HCoVs) in Queensland children with acute respiratory tract illnesses in 2004. AU - Mackay,Ian M, AU - Arden,Katherine E, AU - Speicher,David J, AU - O'Neil,Nicholas T, AU - McErlean,Peter K, AU - Greer,Ristan M, AU - Nissen,Michael D, AU - Sloots,Theo P, Y1 - 2012/04/23/ PY - 2012/03/23/received PY - 2012/04/11/revised PY - 2012/04/11/accepted PY - 2012/5/17/entrez PY - 2012/5/17/pubmed PY - 2012/9/6/medline KW - HCoV-229E KW - HCoV-HKU1 KW - HCoV-NL63 KW - HCoV-OC43 KW - clinical impact KW - coronavirus KW - real-time PCR KW - respiratory virus SP - 637 EP - 53 JF - Viruses JO - Viruses VL - 4 IS - 4 N2 - Acute respiratory illnesses (ARIs) with unconfirmed infectious aetiologies peak at different times of the year. Molecular diagnostic assays reduce the number of unconfirmed ARIs compared to serology- or culture-based techniques. Screening of 888 inpatient and outpatient respiratory specimens spanning late autumn through to early spring, 2004, identified the presence of a human coronavirus (HCoV) on 74 occasions (8.3% of all specimens and 26.3% of all respiratory virus detections). Prevalence peaked in August (late winter in the southern hemisphere) when they were detected in 21.9% of specimens tested. HCoV-HKU1 and HCoV-OC43 comprised 82.4% of all HCoVs detected. Positive specimens were used to develop novel reverse transcriptase real-time PCRs (RT-rtPCRs) for HCoV detection. An objective clinical severity score was assigned to each positive HCoV patient. Severity scores were similar to those from a random selection of young children who were positive for respiratory syncytial virus at a different time but from the same specimen population. During the cooler months of 2004, sensitive and specific RT-rtPCRs identified the concurrent circulation of all four HCoVs, a quarter of which co-occurred with another virus and most of which were from children under the age of two years. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/22590689/Co_circulation_of_four_human_coronaviruses__HCoVs__in_Queensland_children_with_acute_respiratory_tract_illnesses_in_2004_ L2 - https://www.mdpi.com/resolver?pii=v4040637 DB - PRIME DP - Unbound Medicine ER -