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A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae.
Funct Integr Genomics. 2012 Jun; 12(2):215-28.FI

Abstract

The dominant rice blast resistance gene, Pi54 confers resistance to Magnaporthe oryzae in different parts of India. In our effort to identify more effective forms of this gene, we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice, Oryza rhizomatis, using allele mining approach and validated by complementation. The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger (C(3)H type) domain. The 1,447 bp Pi54rh transcript comprises of 101 bp 5'-UTR, 1,083 bp coding region and 263 bp 3'-UTR, driven by pathogen inducible promoter. We showed the extracellular localization of Pi54rh protein and the presence of glycosylation, myristoylation and phosphorylation sites which implicates its role in signal transduction process. This is in contrast to other blast resistance genes that are predicted to be intracellular NBS-LRR-type resistance proteins. The Pi54rh was found to express constitutively at basal level in the leaves, but upregulates 3.8-fold at 96 h post-inoculation with the pathogen. Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M. oryzae collected from different geographical locations of India. In this study, for the first time, we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M. oryzae hence can be used in rice improvement breeding programme.

Authors+Show Affiliations

National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22592658

Citation

Das, Alok, et al. "A Novel Blast Resistance Gene, Pi54rh Cloned From Wild Species of Rice, Oryza Rhizomatis Confers Broad Spectrum Resistance to Magnaporthe Oryzae." Functional & Integrative Genomics, vol. 12, no. 2, 2012, pp. 215-28.
Das A, Soubam D, Singh PK, et al. A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae. Funct Integr Genomics. 2012;12(2):215-28.
Das, A., Soubam, D., Singh, P. K., Thakur, S., Singh, N. K., & Sharma, T. R. (2012). A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae. Functional & Integrative Genomics, 12(2), 215-28. https://doi.org/10.1007/s10142-012-0284-1
Das A, et al. A Novel Blast Resistance Gene, Pi54rh Cloned From Wild Species of Rice, Oryza Rhizomatis Confers Broad Spectrum Resistance to Magnaporthe Oryzae. Funct Integr Genomics. 2012;12(2):215-28. PubMed PMID: 22592658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel blast resistance gene, Pi54rh cloned from wild species of rice, Oryza rhizomatis confers broad spectrum resistance to Magnaporthe oryzae. AU - Das,Alok, AU - Soubam,D, AU - Singh,P K, AU - Thakur,S, AU - Singh,N K, AU - Sharma,T R, Y1 - 2012/05/17/ PY - 2012/02/09/received PY - 2012/04/30/accepted PY - 2012/04/21/revised PY - 2012/5/18/entrez PY - 2012/5/18/pubmed PY - 2012/9/29/medline SP - 215 EP - 28 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 12 IS - 2 N2 - The dominant rice blast resistance gene, Pi54 confers resistance to Magnaporthe oryzae in different parts of India. In our effort to identify more effective forms of this gene, we isolated an orthologue of Pi54 named as Pi54rh from the blast-resistant wild species of rice, Oryza rhizomatis, using allele mining approach and validated by complementation. The Pi54rh belongs to CC-NBS-LRR family of disease resistance genes with a unique Zinc finger (C(3)H type) domain. The 1,447 bp Pi54rh transcript comprises of 101 bp 5'-UTR, 1,083 bp coding region and 263 bp 3'-UTR, driven by pathogen inducible promoter. We showed the extracellular localization of Pi54rh protein and the presence of glycosylation, myristoylation and phosphorylation sites which implicates its role in signal transduction process. This is in contrast to other blast resistance genes that are predicted to be intracellular NBS-LRR-type resistance proteins. The Pi54rh was found to express constitutively at basal level in the leaves, but upregulates 3.8-fold at 96 h post-inoculation with the pathogen. Functional validation of cloned Pi54rh gene using complementation test showed high degree of resistance to seven isolates of M. oryzae collected from different geographical locations of India. In this study, for the first time, we demonstrated that a rice blast resistance gene Pi54rh cloned from wild species of rice provides broad spectrum resistance to M. oryzae hence can be used in rice improvement breeding programme. SN - 1438-7948 UR - https://www.unboundmedicine.com/medline/citation/22592658/A_novel_blast_resistance_gene_Pi54rh_cloned_from_wild_species_of_rice_Oryza_rhizomatis_confers_broad_spectrum_resistance_to_Magnaporthe_oryzae_ L2 - https://dx.doi.org/10.1007/s10142-012-0284-1 DB - PRIME DP - Unbound Medicine ER -