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Effect of age on the pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses.
Virus Res. 2012 Aug; 167(2):196-206.VR

Abstract

The pathogenicity of H5N1 highly pathogenic avian influenza (HPAI) viruses in domestic ducks varies between different viruses and is affected by the age of the ducks, with younger ducks presenting a more severe disease. In order to better understand the pathobiology of H5N1 HPAI in ducks including the role of host responses, 2 and 5-week-old Pekin ducks were infected with three different H5N1 HPAI viruses. Virus-induced pathology ranged from no clinical signs to severe disease and mortality, with the 2-week-old ducks being more severely affected by the more virulent viruses. However, these more virulent viruses induced higher body temperatures in the 5-week-old ducks than in the 2-week-old ducks indicating possible differences in innate immune responses. To analyze the ducks host responses to H5N1 HPAI virus infection, expression of innate immune-related genes was measured in the spleens and lungs of infected ducks at the peak of virus infection. IFN-α, RIG-I, and IL-6 RNA levels were increased in spleens regardless of the virus given and the age of the ducks, however differences were observed in the levels of up-regulation of IFN-α and RIG-I between the 2 and the 5-week-old ducks with the more virulent virus. Differences in IL-2 gene expression were also observed. In the lungs, the levels of expression of innate immune-related genes were lower than in the spleen, with mostly up-regulation of RIG-I and IL-6 and down-regulation of IFN-α and IL-2; no significant difference in expression was found between the 2 and the 5-week-old ducks. The differences observed in the innate immune responses to infection with H5N1 HPAI viruses could explain in part the differences in pathogenicity found between the 2 and 5-week-old ducks, however earlier time points after infection and additional innate immune-related genes should be examined.

Authors+Show Affiliations

Exotic and Emerging Avian Viral Diseases Research Unit, Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA. mary.pantin-jackwood@ars.usda.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22595263

Citation

Pantin-Jackwood, Mary J., et al. "Effect of Age On the Pathogenesis and Innate Immune Responses in Pekin Ducks Infected With Different H5N1 Highly Pathogenic Avian Influenza Viruses." Virus Research, vol. 167, no. 2, 2012, pp. 196-206.
Pantin-Jackwood MJ, Smith DM, Wasilenko JL, et al. Effect of age on the pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses. Virus Res. 2012;167(2):196-206.
Pantin-Jackwood, M. J., Smith, D. M., Wasilenko, J. L., Cagle, C., Shepherd, E., Sarmento, L., Kapczynski, D. R., & Afonso, C. L. (2012). Effect of age on the pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses. Virus Research, 167(2), 196-206. https://doi.org/10.1016/j.virusres.2012.04.015
Pantin-Jackwood MJ, et al. Effect of Age On the Pathogenesis and Innate Immune Responses in Pekin Ducks Infected With Different H5N1 Highly Pathogenic Avian Influenza Viruses. Virus Res. 2012;167(2):196-206. PubMed PMID: 22595263.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of age on the pathogenesis and innate immune responses in Pekin ducks infected with different H5N1 highly pathogenic avian influenza viruses. AU - Pantin-Jackwood,Mary J, AU - Smith,Diane M, AU - Wasilenko,Jamie L, AU - Cagle,Caran, AU - Shepherd,Eric, AU - Sarmento,Luciana, AU - Kapczynski,Darrell R, AU - Afonso,Claudio L, Y1 - 2012/05/15/ PY - 2011/12/16/received PY - 2012/04/11/revised PY - 2012/04/27/accepted PY - 2012/5/19/entrez PY - 2012/5/19/pubmed PY - 2012/11/9/medline SP - 196 EP - 206 JF - Virus research JO - Virus Res VL - 167 IS - 2 N2 - The pathogenicity of H5N1 highly pathogenic avian influenza (HPAI) viruses in domestic ducks varies between different viruses and is affected by the age of the ducks, with younger ducks presenting a more severe disease. In order to better understand the pathobiology of H5N1 HPAI in ducks including the role of host responses, 2 and 5-week-old Pekin ducks were infected with three different H5N1 HPAI viruses. Virus-induced pathology ranged from no clinical signs to severe disease and mortality, with the 2-week-old ducks being more severely affected by the more virulent viruses. However, these more virulent viruses induced higher body temperatures in the 5-week-old ducks than in the 2-week-old ducks indicating possible differences in innate immune responses. To analyze the ducks host responses to H5N1 HPAI virus infection, expression of innate immune-related genes was measured in the spleens and lungs of infected ducks at the peak of virus infection. IFN-α, RIG-I, and IL-6 RNA levels were increased in spleens regardless of the virus given and the age of the ducks, however differences were observed in the levels of up-regulation of IFN-α and RIG-I between the 2 and the 5-week-old ducks with the more virulent virus. Differences in IL-2 gene expression were also observed. In the lungs, the levels of expression of innate immune-related genes were lower than in the spleen, with mostly up-regulation of RIG-I and IL-6 and down-regulation of IFN-α and IL-2; no significant difference in expression was found between the 2 and the 5-week-old ducks. The differences observed in the innate immune responses to infection with H5N1 HPAI viruses could explain in part the differences in pathogenicity found between the 2 and 5-week-old ducks, however earlier time points after infection and additional innate immune-related genes should be examined. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/22595263/Effect_of_age_on_the_pathogenesis_and_innate_immune_responses_in_Pekin_ducks_infected_with_different_H5N1_highly_pathogenic_avian_influenza_viruses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(12)00155-4 DB - PRIME DP - Unbound Medicine ER -