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Biochemical basis of organophosphate and carbamate resistance in Asian citrus psyllid.
J Econ Entomol. 2012 Apr; 105(2):540-8.JE

Abstract

The Asian citrus psyllid, Diaphorina citri Kuwayama, is a worldwide pest of citrus, which vectors the putative causal pathogen of huanglongbing. Current management practices warrant continuous monitoring of field populations for insecticide resistance. Baseline activities of acetylcholinesterase (AChE), general esterase, and glutathione S-transferase as well as sensitivity of AChE to selected organophosphate and carbamate insecticides were established for a susceptible laboratory strain (Lab) and compared with several field populations of D. citri from Florida. The specific activity of AChE in various D. citri populations ranged from 0.77 to 1.29 microM min(-1) mg of protein(-1); the Lab strain was characterized by the highest activity. Although reduced AChE sensitivity was observed in the Lab strain compared with field populations, overlap of 95% confidence intervals of I50 values (concentration required for 50% AChE activity inhibition) suggests no significant difference in AChE sensitivity among all populations tested for a given insecticide. There was no significant evidence of target site insensitivity in field populations that were exposed to the selected organophosphate and carbamate insecticides tested. The specific activity of general esterase and glutathione S-transferase was lowest in the Lab strain and was generally comparable to that of the field populations evaluated. The current data provide a mode-of-action specific baseline for future monitoring of resistance to organophosphate and carbamate insecticides in populations of D. citri.

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Authors+Show Affiliations

Tiwari S
Entomology and Nematology Department, Citrus Research and Education Center, University of Florida, 700 Experiment Station Road, Lake Alfred, FL 33850, USA. stiwari@ufl.edu
Stelinski LL
No affiliation info available
Rogers ME
No affiliation info available

MeSH

AcetylcholinesteraseAnimalsCarbamatesCitrusEsterasesFloridaGlutathione TransferaseHemipteraInsect ProteinsInsecticide ResistanceInsecticidesNymphOrganophosphorus Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22606825

Citation

Tiwari, Siddharth, et al. "Biochemical Basis of Organophosphate and Carbamate Resistance in Asian Citrus Psyllid." Journal of Economic Entomology, vol. 105, no. 2, 2012, pp. 540-8.
Tiwari S, Stelinski LL, Rogers ME. Biochemical basis of organophosphate and carbamate resistance in Asian citrus psyllid. J Econ Entomol. 2012;105(2):540-8.
Tiwari, S., Stelinski, L. L., & Rogers, M. E. (2012). Biochemical basis of organophosphate and carbamate resistance in Asian citrus psyllid. Journal of Economic Entomology, 105(2), 540-8.
Tiwari S, Stelinski LL, Rogers ME. Biochemical Basis of Organophosphate and Carbamate Resistance in Asian Citrus Psyllid. J Econ Entomol. 2012;105(2):540-8. PubMed PMID: 22606825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biochemical basis of organophosphate and carbamate resistance in Asian citrus psyllid. AU - Tiwari,Siddharth, AU - Stelinski,Lukasz L, AU - Rogers,Michael E, PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/6/8/medline SP - 540 EP - 8 JF - Journal of economic entomology JO - J Econ Entomol VL - 105 IS - 2 N2 - The Asian citrus psyllid, Diaphorina citri Kuwayama, is a worldwide pest of citrus, which vectors the putative causal pathogen of huanglongbing. Current management practices warrant continuous monitoring of field populations for insecticide resistance. Baseline activities of acetylcholinesterase (AChE), general esterase, and glutathione S-transferase as well as sensitivity of AChE to selected organophosphate and carbamate insecticides were established for a susceptible laboratory strain (Lab) and compared with several field populations of D. citri from Florida. The specific activity of AChE in various D. citri populations ranged from 0.77 to 1.29 microM min(-1) mg of protein(-1); the Lab strain was characterized by the highest activity. Although reduced AChE sensitivity was observed in the Lab strain compared with field populations, overlap of 95% confidence intervals of I50 values (concentration required for 50% AChE activity inhibition) suggests no significant difference in AChE sensitivity among all populations tested for a given insecticide. There was no significant evidence of target site insensitivity in field populations that were exposed to the selected organophosphate and carbamate insecticides tested. The specific activity of general esterase and glutathione S-transferase was lowest in the Lab strain and was generally comparable to that of the field populations evaluated. The current data provide a mode-of-action specific baseline for future monitoring of resistance to organophosphate and carbamate insecticides in populations of D. citri. SN - 0022-0493 UR - https://www.unboundmedicine.com/medline/citation/22606825/Biochemical_basis_of_organophosphate_and_carbamate_resistance_in_Asian_citrus_psyllid_ L2 - https://academic.oup.com/jee/article-lookup/doi/10.1603/ec11228 DB - PRIME DP - Unbound Medicine ER -