Tags

Type your tag names separated by a space and hit enter

The role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the host response to mixed infection.
J Clin Periodontol. 2012 Jul; 39(7):617-25.JC

Abstract

AIM

To evaluate the role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the virulence of the mixed infection in mice.

MATERIALS AND METHODS

Inhibition of coaggregation was carried out using lactose. In vitro, inhibition of coaggregation was verified using a coaggregation assay. In vivo, the virulence of the mixed infection, with and without coaggregation, was examined in a model of experimental periodontitis in mice. The local host response to the mixed infection, with or without coaggregation, was examined using the subcutaneous chamber model of infection.

RESULTS

Lactose inhibited the coaggregation between P. gingivalis and F. nucleatum at all the tested concentrations (1-0.0625 M). Surprisingly, the addition of lactose to the mixed infection increased the severity of experimental periodontitis (as measured by alveolar bone loss) compared with mixed infection with coaggregating bacteria. The addition of lactose to the mixed infection resulted in mild attenuation of TNFα and IL-1β levels. In addition, inhibition of coaggregation resulted in inhibition of the phagocytosis of F. nucleatum and augmentation of the phagocytosis of P. gingivalis.

CONCLUSIONS

The ability of P. gingivalis and F. nucleatum to coaggregate may limit their ability to induce experimental periodontitis in a mixed infection model. Moreover, there is a shift in the phagocytosis pattern of the bacteria with the annulment of coaggregeaiton, with a reduction in F. nucleatum phagocytosis and amplification of P. gingivalis phagocytosis. The increased virulence of the mixed infection without coaggregation may surprisingly lay in the sustention of F. nucleatum in the infected sites.

Authors+Show Affiliations

Department of Periodontology, The Hebrew University-Hadassah Medical center, Jerusalem, Israel. polak@cc.huji.ac.ilNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22607053

Citation

Polak, David, et al. "The Role of Coaggregation Between Porphyromonas Gingivalis and Fusobacterium Nucleatum On the Host Response to Mixed Infection." Journal of Clinical Periodontology, vol. 39, no. 7, 2012, pp. 617-25.
Polak D, Shapira L, Weiss EI, et al. The role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the host response to mixed infection. J Clin Periodontol. 2012;39(7):617-25.
Polak, D., Shapira, L., Weiss, E. I., & Houri-Haddad, Y. (2012). The role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the host response to mixed infection. Journal of Clinical Periodontology, 39(7), 617-25. https://doi.org/10.1111/j.1600-051X.2012.01889.x
Polak D, et al. The Role of Coaggregation Between Porphyromonas Gingivalis and Fusobacterium Nucleatum On the Host Response to Mixed Infection. J Clin Periodontol. 2012;39(7):617-25. PubMed PMID: 22607053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the host response to mixed infection. AU - Polak,David, AU - Shapira,Lior, AU - Weiss,Ervin I, AU - Houri-Haddad,Yael, Y1 - 2012/05/21/ PY - 2012/03/24/accepted PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/12/10/medline SP - 617 EP - 25 JF - Journal of clinical periodontology JO - J Clin Periodontol VL - 39 IS - 7 N2 - AIM: To evaluate the role of coaggregation between Porphyromonas gingivalis and Fusobacterium nucleatum on the virulence of the mixed infection in mice. MATERIALS AND METHODS: Inhibition of coaggregation was carried out using lactose. In vitro, inhibition of coaggregation was verified using a coaggregation assay. In vivo, the virulence of the mixed infection, with and without coaggregation, was examined in a model of experimental periodontitis in mice. The local host response to the mixed infection, with or without coaggregation, was examined using the subcutaneous chamber model of infection. RESULTS: Lactose inhibited the coaggregation between P. gingivalis and F. nucleatum at all the tested concentrations (1-0.0625 M). Surprisingly, the addition of lactose to the mixed infection increased the severity of experimental periodontitis (as measured by alveolar bone loss) compared with mixed infection with coaggregating bacteria. The addition of lactose to the mixed infection resulted in mild attenuation of TNFα and IL-1β levels. In addition, inhibition of coaggregation resulted in inhibition of the phagocytosis of F. nucleatum and augmentation of the phagocytosis of P. gingivalis. CONCLUSIONS: The ability of P. gingivalis and F. nucleatum to coaggregate may limit their ability to induce experimental periodontitis in a mixed infection model. Moreover, there is a shift in the phagocytosis pattern of the bacteria with the annulment of coaggregeaiton, with a reduction in F. nucleatum phagocytosis and amplification of P. gingivalis phagocytosis. The increased virulence of the mixed infection without coaggregation may surprisingly lay in the sustention of F. nucleatum in the infected sites. SN - 1600-051X UR - https://www.unboundmedicine.com/medline/citation/22607053/The_role_of_coaggregation_between_Porphyromonas_gingivalis_and_Fusobacterium_nucleatum_on_the_host_response_to_mixed_infection_ L2 - https://doi.org/10.1111/j.1600-051X.2012.01889.x DB - PRIME DP - Unbound Medicine ER -