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Response of mitochondrial antioxidant system and respiratory pathways to reactive nitrogen species in pea leaves.
Physiol Plant. 2013 Feb; 147(2):194-206.PP

Abstract

Nitric oxide (NO) has emerged as an important signaling molecule in plants, but little is known about the effects of reactive nitrogen species in plant mitochondria. In this study, the effects of DETA-NONOate, a pure NO slow generator, and of SIN-1 (3-morpholinosydnonimine), a peroxynitrite producer, on the activities of respiratory pathways, enzymatic and non-enzymatic antioxidants have been investigated in isolated mitochondria from pea leaves. No significant changes in lipid peroxidation, protein oxidation or in ascorbate and glutathione redox state were observed after DETA-NONOate treatments whereas cytochrome pathway (CP) respiration was reversibly inhibited and alternative pathway (AP) respiration showed little inhibition. On the other hand, NO did not affect neither activities of Mn superoxide dismutase (Mn-SOD) nor enzymes involved in the ascorbate and glutathione regeneration in mitochondria except for ascorbate peroxidase (APX), which was reversely inhibited depending on ascorbate concentration. Finally, SIN-1 treatment of mitochondria produced a decrease in CP respiration, an increase in protein oxidation and strongly inhibited APX activity (90%), with glutathione reductase and dehydroascorbate reductase (DHAR) being moderately inhibited (30 and 20%, respectively). This treatment did not affect monodehydroascorbate reductase (MDHAR) and Mn-SOD activities. Results showed that mitochondrial nitrosative stress was not necessarily accompanied by oxidative stress. We suggest that NO-resistant AP and mitochondrial APX may be important components of the H(2) O(2) -signaling pathways under nitrosative stress induced by NO in this organelle. Also, MDHAR and DHAR, via ascorbate regeneration, could constitute an essential antioxidant defense together with Mn-SOD, against NO and ONOO(-) stress in plant mitochondria.

Authors+Show Affiliations

Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22607494

Citation

Martí, María C., et al. "Response of Mitochondrial Antioxidant System and Respiratory Pathways to Reactive Nitrogen Species in Pea Leaves." Physiologia Plantarum, vol. 147, no. 2, 2013, pp. 194-206.
Martí MC, Florez-Sarasa I, Camejo D, et al. Response of mitochondrial antioxidant system and respiratory pathways to reactive nitrogen species in pea leaves. Physiol Plant. 2013;147(2):194-206.
Martí, M. C., Florez-Sarasa, I., Camejo, D., Pallol, B., Ortiz, A., Ribas-Carbó, M., Jiménez, A., & Sevilla, F. (2013). Response of mitochondrial antioxidant system and respiratory pathways to reactive nitrogen species in pea leaves. Physiologia Plantarum, 147(2), 194-206. https://doi.org/10.1111/j.1399-3054.2012.01654.x
Martí MC, et al. Response of Mitochondrial Antioxidant System and Respiratory Pathways to Reactive Nitrogen Species in Pea Leaves. Physiol Plant. 2013;147(2):194-206. PubMed PMID: 22607494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Response of mitochondrial antioxidant system and respiratory pathways to reactive nitrogen species in pea leaves. AU - Martí,María C, AU - Florez-Sarasa,Igor, AU - Camejo,Daymi, AU - Pallol,Beatriz, AU - Ortiz,Ana, AU - Ribas-Carbó,Miquel, AU - Jiménez,Ana, AU - Sevilla,Francisca, Y1 - 2012/06/27/ PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2013/8/1/medline SP - 194 EP - 206 JF - Physiologia plantarum JO - Physiol Plant VL - 147 IS - 2 N2 - Nitric oxide (NO) has emerged as an important signaling molecule in plants, but little is known about the effects of reactive nitrogen species in plant mitochondria. In this study, the effects of DETA-NONOate, a pure NO slow generator, and of SIN-1 (3-morpholinosydnonimine), a peroxynitrite producer, on the activities of respiratory pathways, enzymatic and non-enzymatic antioxidants have been investigated in isolated mitochondria from pea leaves. No significant changes in lipid peroxidation, protein oxidation or in ascorbate and glutathione redox state were observed after DETA-NONOate treatments whereas cytochrome pathway (CP) respiration was reversibly inhibited and alternative pathway (AP) respiration showed little inhibition. On the other hand, NO did not affect neither activities of Mn superoxide dismutase (Mn-SOD) nor enzymes involved in the ascorbate and glutathione regeneration in mitochondria except for ascorbate peroxidase (APX), which was reversely inhibited depending on ascorbate concentration. Finally, SIN-1 treatment of mitochondria produced a decrease in CP respiration, an increase in protein oxidation and strongly inhibited APX activity (90%), with glutathione reductase and dehydroascorbate reductase (DHAR) being moderately inhibited (30 and 20%, respectively). This treatment did not affect monodehydroascorbate reductase (MDHAR) and Mn-SOD activities. Results showed that mitochondrial nitrosative stress was not necessarily accompanied by oxidative stress. We suggest that NO-resistant AP and mitochondrial APX may be important components of the H(2) O(2) -signaling pathways under nitrosative stress induced by NO in this organelle. Also, MDHAR and DHAR, via ascorbate regeneration, could constitute an essential antioxidant defense together with Mn-SOD, against NO and ONOO(-) stress in plant mitochondria. SN - 1399-3054 UR - https://www.unboundmedicine.com/medline/citation/22607494/Response_of_mitochondrial_antioxidant_system_and_respiratory_pathways_to_reactive_nitrogen_species_in_pea_leaves_ L2 - https://doi.org/10.1111/j.1399-3054.2012.01654.x DB - PRIME DP - Unbound Medicine ER -