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RNA fingerprinting analysis of Oenococcus oeni strains under wine conditions.
Food Microbiol. 2012 Sep; 31(2):238-45.FM

Abstract

Oenococcus oeni is a lactic acid bacterium of economic interest used in winemaking. This bacterium is the preferred species for malolactic fermentation (MLF) due its adaptability to the chemically harsh wine environment. MLF enhances the organoleptic properties and ensures deacidification of wines. The aim of this work was the transcriptional characterization of six O. oeni strains, four of them selected from distinct winemaking regions of Portugal, as candidates to malolactic starters, and two commercial malolactic starters. Using crossed assays with wines from different Portuguese winemaking regions, strain characteristic transcriptional patterns induced by each wine were analyzed based on Random Arbitrarily Primed PCR (RAP-PCR). The obtained results suggest that the starter strains showed more constrained and limited transcription profiles, whereas a high variation on the distribution of the transcription profiles was observed for the regional strains in each wine. According with our results, RAP-PCR is a useful technique for a preliminary investigation of strain behavior under different wine environmental conditions, which can be applied in field studies to monitor differential patterns of global gene expression and to select markers for the surveillance of malolactic starters performance in winemaking, as well as for quality and safety control.

Authors+Show Affiliations

Instituto de Biologia Experimental e Tecnológica (IBET), Apartado 12, 2781-901 Oeiras, Portugal. amarques@itqb.unl.ptNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22608229

Citation

Marques, Ana Paula, et al. "RNA Fingerprinting Analysis of Oenococcus Oeni Strains Under Wine Conditions." Food Microbiology, vol. 31, no. 2, 2012, pp. 238-45.
Marques AP, San Romão MV, Tenreiro R. RNA fingerprinting analysis of Oenococcus oeni strains under wine conditions. Food Microbiol. 2012;31(2):238-45.
Marques, A. P., San Romão, M. V., & Tenreiro, R. (2012). RNA fingerprinting analysis of Oenococcus oeni strains under wine conditions. Food Microbiology, 31(2), 238-45. https://doi.org/10.1016/j.fm.2012.02.006
Marques AP, San Romão MV, Tenreiro R. RNA Fingerprinting Analysis of Oenococcus Oeni Strains Under Wine Conditions. Food Microbiol. 2012;31(2):238-45. PubMed PMID: 22608229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNA fingerprinting analysis of Oenococcus oeni strains under wine conditions. AU - Marques,Ana Paula, AU - San Romão,Maria Vitória, AU - Tenreiro,Rogério, Y1 - 2012/03/15/ PY - 2011/11/29/received PY - 2012/02/03/revised PY - 2012/02/13/accepted PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/10/12/medline SP - 238 EP - 45 JF - Food microbiology JO - Food Microbiol. VL - 31 IS - 2 N2 - Oenococcus oeni is a lactic acid bacterium of economic interest used in winemaking. This bacterium is the preferred species for malolactic fermentation (MLF) due its adaptability to the chemically harsh wine environment. MLF enhances the organoleptic properties and ensures deacidification of wines. The aim of this work was the transcriptional characterization of six O. oeni strains, four of them selected from distinct winemaking regions of Portugal, as candidates to malolactic starters, and two commercial malolactic starters. Using crossed assays with wines from different Portuguese winemaking regions, strain characteristic transcriptional patterns induced by each wine were analyzed based on Random Arbitrarily Primed PCR (RAP-PCR). The obtained results suggest that the starter strains showed more constrained and limited transcription profiles, whereas a high variation on the distribution of the transcription profiles was observed for the regional strains in each wine. According with our results, RAP-PCR is a useful technique for a preliminary investigation of strain behavior under different wine environmental conditions, which can be applied in field studies to monitor differential patterns of global gene expression and to select markers for the surveillance of malolactic starters performance in winemaking, as well as for quality and safety control. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/22608229/RNA_fingerprinting_analysis_of_Oenococcus_oeni_strains_under_wine_conditions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(12)00043-3 DB - PRIME DP - Unbound Medicine ER -