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Comparison of sensing strategies in SPR biosensor for rapid and sensitive enumeration of bacteria.
Biosens Bioelectron. 2012 Aug-Sep; 37(1):53-60.BB

Abstract

Rapid and sensitive detections of microorganisms are very important for biodefence, food safety, medical diagnosis and pharmaceutics. The present study aims to find out the most proper bioactive surface preparation method to develop rapid, sensitive and selective bacteria biosensor, based on surface plasmon resonance (SPR) spectroscopy. Escherichia coli (E. coli) was used as a model bacterium and four sensing strategies in SPR were tested. Three of these strategies are antibody immobilization methods that are non-specific adsorption, specific adsorption via the avidin-biotin interaction, and immobilization of antibodies via self-assembled monolayer formation. The fourth strategy is a novel method for bacteria enumeration based on the combination of the SPR spectroscopy and immunomagnetic separation with using gold-coated magnetic nanoparticles. According to results, the most efficient SPR method is the one based on gold-coated magnetic nanoparticles. This method allows to specifically separate E. coli from the environment and to quantify rapidly without any labeling procedure. The developed method has a linear range between 30 and 3.0 × 10(4)cfu/ml, and a detection limit of 3 cfu/ml. The selectivity of the method was examined with Enterobacter aerogenes and Enterobacter dissolvens, which did not produce any significant response. The usefulness of the method to detect E. coli in real water samples was also investigated, and the results were compared with the results from plate-counting method. There was no significant difference between the methods (p>0.05).

Authors+Show Affiliations

Hacettepe University, Faculty of Engineering, Department of Food Engineering, Beytepe 06800 Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22608765

Citation

Torun, Ozlem, et al. "Comparison of Sensing Strategies in SPR Biosensor for Rapid and Sensitive Enumeration of Bacteria." Biosensors & Bioelectronics, vol. 37, no. 1, 2012, pp. 53-60.
Torun O, Hakkı Boyacı I, Temür E, et al. Comparison of sensing strategies in SPR biosensor for rapid and sensitive enumeration of bacteria. Biosens Bioelectron. 2012;37(1):53-60.
Torun, O., Hakkı Boyacı, I., Temür, E., & Tamer, U. (2012). Comparison of sensing strategies in SPR biosensor for rapid and sensitive enumeration of bacteria. Biosensors & Bioelectronics, 37(1), 53-60. https://doi.org/10.1016/j.bios.2012.04.034
Torun O, et al. Comparison of Sensing Strategies in SPR Biosensor for Rapid and Sensitive Enumeration of Bacteria. Biosens Bioelectron. 2012 Aug-Sep;37(1):53-60. PubMed PMID: 22608765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of sensing strategies in SPR biosensor for rapid and sensitive enumeration of bacteria. AU - Torun,Ozlem, AU - Hakkı Boyacı,Ismail, AU - Temür,Erhan, AU - Tamer,Uğur, Y1 - 2012/05/06/ PY - 2012/01/24/received PY - 2012/03/30/revised PY - 2012/04/18/accepted PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/9/25/medline SP - 53 EP - 60 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 37 IS - 1 N2 - Rapid and sensitive detections of microorganisms are very important for biodefence, food safety, medical diagnosis and pharmaceutics. The present study aims to find out the most proper bioactive surface preparation method to develop rapid, sensitive and selective bacteria biosensor, based on surface plasmon resonance (SPR) spectroscopy. Escherichia coli (E. coli) was used as a model bacterium and four sensing strategies in SPR were tested. Three of these strategies are antibody immobilization methods that are non-specific adsorption, specific adsorption via the avidin-biotin interaction, and immobilization of antibodies via self-assembled monolayer formation. The fourth strategy is a novel method for bacteria enumeration based on the combination of the SPR spectroscopy and immunomagnetic separation with using gold-coated magnetic nanoparticles. According to results, the most efficient SPR method is the one based on gold-coated magnetic nanoparticles. This method allows to specifically separate E. coli from the environment and to quantify rapidly without any labeling procedure. The developed method has a linear range between 30 and 3.0 × 10(4)cfu/ml, and a detection limit of 3 cfu/ml. The selectivity of the method was examined with Enterobacter aerogenes and Enterobacter dissolvens, which did not produce any significant response. The usefulness of the method to detect E. coli in real water samples was also investigated, and the results were compared with the results from plate-counting method. There was no significant difference between the methods (p>0.05). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22608765/Comparison_of_sensing_strategies_in_SPR_biosensor_for_rapid_and_sensitive_enumeration_of_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00261-8 DB - PRIME DP - Unbound Medicine ER -