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Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway.
Neurochem Int. 2012 Jul; 61(2):251-7.NI

Abstract

Eriodictyol, a flavonoid isolated from the Chinese herb Dracocephalum rupestre has long been established as an antioxidant. The present study was designed to explore the protective effects of eriodictyol against hydrogen peroxide (H(2)O(2))-induced neurotoxicity with cultured rat pheochromocytoma cells (PC12 cells) and the possible mechanisms involved. For this purpose, differentiated PC12 cells were cultured and exposed to 200 μM H(2)O(2) in the absence or presence of eriodictyol (20, 40 and 80 μM). In addition, the potential contribution of the Nrf2/ARE neuroprotective pathway in eriodictyol-mediated protection against H(2)O(2)-induced neurotoxicity was also investigated. The results showed that H(2)O(2)-induced cell death can be inhibited in the presence of eriodictyol as measured by assays for MTT and apoptosis. Further study revealed that eriodictyol induced the nuclear translocation of Nrf2, enhanced the expression of heme oxygenase (HO-1) and γ-glutamylcysteine synthetase (γ-GCS), and increased the levels of intracellular glutathione. Treatment of PC12 cells with Nrf2 small interference RNA abolished eriodictyol-induced HO-1 and γ-GCS expression and its protective effects. In conclusion, these results suggest that eriodictyol upregulates HO-1 and γ-GCS expression through the activation of Nrf2/ARE pathway and protects PC12 cells against H(2)O(2)-induced oxidative stress.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Shandong University, Jinan 250012, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22609376

Citation

Lou, Haiyan, et al. "Eriodictyol Protects Against H(2)O(2)-induced Neuron-like PC12 Cell Death Through Activation of Nrf2/ARE Signaling Pathway." Neurochemistry International, vol. 61, no. 2, 2012, pp. 251-7.
Lou H, Jing X, Ren D, et al. Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway. Neurochem Int. 2012;61(2):251-7.
Lou, H., Jing, X., Ren, D., Wei, X., & Zhang, X. (2012). Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway. Neurochemistry International, 61(2), 251-7. https://doi.org/10.1016/j.neuint.2012.05.013
Lou H, et al. Eriodictyol Protects Against H(2)O(2)-induced Neuron-like PC12 Cell Death Through Activation of Nrf2/ARE Signaling Pathway. Neurochem Int. 2012;61(2):251-7. PubMed PMID: 22609376.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway. AU - Lou,Haiyan, AU - Jing,Xu, AU - Ren,Dongmei, AU - Wei,Xinbing, AU - Zhang,Xiumei, Y1 - 2012/05/17/ PY - 2011/12/08/received PY - 2012/05/04/revised PY - 2012/05/09/accepted PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/12/12/medline SP - 251 EP - 7 JF - Neurochemistry international JO - Neurochem Int VL - 61 IS - 2 N2 - Eriodictyol, a flavonoid isolated from the Chinese herb Dracocephalum rupestre has long been established as an antioxidant. The present study was designed to explore the protective effects of eriodictyol against hydrogen peroxide (H(2)O(2))-induced neurotoxicity with cultured rat pheochromocytoma cells (PC12 cells) and the possible mechanisms involved. For this purpose, differentiated PC12 cells were cultured and exposed to 200 μM H(2)O(2) in the absence or presence of eriodictyol (20, 40 and 80 μM). In addition, the potential contribution of the Nrf2/ARE neuroprotective pathway in eriodictyol-mediated protection against H(2)O(2)-induced neurotoxicity was also investigated. The results showed that H(2)O(2)-induced cell death can be inhibited in the presence of eriodictyol as measured by assays for MTT and apoptosis. Further study revealed that eriodictyol induced the nuclear translocation of Nrf2, enhanced the expression of heme oxygenase (HO-1) and γ-glutamylcysteine synthetase (γ-GCS), and increased the levels of intracellular glutathione. Treatment of PC12 cells with Nrf2 small interference RNA abolished eriodictyol-induced HO-1 and γ-GCS expression and its protective effects. In conclusion, these results suggest that eriodictyol upregulates HO-1 and γ-GCS expression through the activation of Nrf2/ARE pathway and protects PC12 cells against H(2)O(2)-induced oxidative stress. SN - 1872-9754 UR - https://www.unboundmedicine.com/medline/citation/22609376/Eriodictyol_protects_against_H_2_O_2__induced_neuron_like_PC12_cell_death_through_activation_of_Nrf2/ARE_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-0186(12)00173-8 DB - PRIME DP - Unbound Medicine ER -