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Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages.
Biosens Bioelectron. 2012 Aug-Sep; 37(1):24-9.BB

Abstract

Early diagnosis and appropriate treatment of Escherichia coli (E. coli) O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) are key elements in preventing resultant life-threatening illnesses, such as hemorrhagic colitis, hemolytic uremic syndrome, and septicemia. In this report, we describe the use of surface plasmon resonance (SPR) for the biodetection of pathogenic bacteria, using bacteriophages as the recognition elements. T4 bacteriophages were used to detect E. coli, while a novel, highly specific phage was used to detect MRSA. We found that the system permits label-free, real-time, specific, rapid and cost-effective detection of pathogens, for concentrations of 10(3) colony forming units/milliliter, in less than 20 min. This system promises to become a diagnostic tool for bacteria that cause major public concern for food safety, bioterrorism, and nosocomial infections.

Authors+Show Affiliations

Regroupement Québecois de Matériaux de Pointe, Département de Génie Physique, École Polytechnique de Montreal, Case Postale 6079, succursale. Centre-Ville, Montreal, Québec, Canada H3C 3A7. nancy.tawil@polymtl.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22609555

Citation

Tawil, Nancy, et al. "Surface Plasmon Resonance Detection of E. Coli and Methicillin-resistant S. Aureus Using Bacteriophages." Biosensors & Bioelectronics, vol. 37, no. 1, 2012, pp. 24-9.
Tawil N, Sacher E, Mandeville R, et al. Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages. Biosens Bioelectron. 2012;37(1):24-9.
Tawil, N., Sacher, E., Mandeville, R., & Meunier, M. (2012). Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages. Biosensors & Bioelectronics, 37(1), 24-9. https://doi.org/10.1016/j.bios.2012.04.048
Tawil N, et al. Surface Plasmon Resonance Detection of E. Coli and Methicillin-resistant S. Aureus Using Bacteriophages. Biosens Bioelectron. 2012 Aug-Sep;37(1):24-9. PubMed PMID: 22609555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages. AU - Tawil,Nancy, AU - Sacher,Edward, AU - Mandeville,Rosemonde, AU - Meunier,Michel, Y1 - 2012/05/11/ PY - 2012/03/02/received PY - 2012/04/11/revised PY - 2012/04/13/accepted PY - 2012/5/22/entrez PY - 2012/5/23/pubmed PY - 2012/9/25/medline SP - 24 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 37 IS - 1 N2 - Early diagnosis and appropriate treatment of Escherichia coli (E. coli) O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) are key elements in preventing resultant life-threatening illnesses, such as hemorrhagic colitis, hemolytic uremic syndrome, and septicemia. In this report, we describe the use of surface plasmon resonance (SPR) for the biodetection of pathogenic bacteria, using bacteriophages as the recognition elements. T4 bacteriophages were used to detect E. coli, while a novel, highly specific phage was used to detect MRSA. We found that the system permits label-free, real-time, specific, rapid and cost-effective detection of pathogens, for concentrations of 10(3) colony forming units/milliliter, in less than 20 min. This system promises to become a diagnostic tool for bacteria that cause major public concern for food safety, bioterrorism, and nosocomial infections. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22609555/Surface_plasmon_resonance_detection_of_E__coli_and_methicillin_resistant_S__aureus_using_bacteriophages_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00278-3 DB - PRIME DP - Unbound Medicine ER -