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Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus.
J Agric Food Chem. 2012 Jun 13; 60(23):5874-83.JA

Abstract

A process for the effective extraction and fractionation of phlorotannins from Fucus vesiculosus with high antioxidant potentials was investigated. The antioxidant activity of F. vesiculosus extract/fractions was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, reducing power, and ferrous ion-chelating assays. Among the crude extract and different polarity fractions, the phlorotannin-enriched ethyl acetate fraction possessed the highest DPPH scavenging activity and reducing power. This fraction was further fractionated by Sephadex LH-20 column chromatography or ultrafiltration. The antioxidant properties were evaluated by both the above chemical antioxidant tests and a mononuclear cell-based bioassay. Sephadex subfractions LH-2 and LH-3 with high total phlorotannin content exhibited strong DPPH quenching activity, comparable to those of ascorbic acid and butylated hydroxytoluene and significantly higher than that of α-tocopherol. Polyphenols in F. vesiculosus were found to consist mainly of high molecular weight phlorotannin polymers. There were no clear relationships between the degree of polymerization, molecular size, and antioxidant activity. All the subfractions separated by Sephadex LH-20 column chromatography and ultrafiltration showed a high ability to scavenge reactive oxygen species generated by mononuclear cells. Further characterization of the phlorotannin compounds was performed on six Sephadex subfractions. Several phlorotannin oligomers were tentatively identified on the basis of HPLC-ESI-MS(n) analyses.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang T
College of Food Science and Engineering, Dalian Ocean University , Heishijiao 52, 116023 Dalian, China.
Jónsdóttir R
No affiliation info available
Liu H
No affiliation info available
Gu L
No affiliation info available
Kristinsson HG
No affiliation info available
Raghavan S
No affiliation info available
Olafsdóttir G
No affiliation info available

MeSH

AntioxidantsChemical FractionationChromatography, High Pressure LiquidDextransMass SpectrometryMolecular WeightPhaeophytaReactive Oxygen SpeciesSeaweedTanninsalpha-Tocopherol

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22612266

Citation

Wang, Tao, et al. "Antioxidant Capacities of Phlorotannins Extracted From the Brown Algae Fucus Vesiculosus." Journal of Agricultural and Food Chemistry, vol. 60, no. 23, 2012, pp. 5874-83.
Wang T, Jónsdóttir R, Liu H, et al. Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus. J Agric Food Chem. 2012;60(23):5874-83.
Wang, T., Jónsdóttir, R., Liu, H., Gu, L., Kristinsson, H. G., Raghavan, S., & Olafsdóttir, G. (2012). Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus. Journal of Agricultural and Food Chemistry, 60(23), 5874-83. https://doi.org/10.1021/jf3003653
Wang T, et al. Antioxidant Capacities of Phlorotannins Extracted From the Brown Algae Fucus Vesiculosus. J Agric Food Chem. 2012 Jun 13;60(23):5874-83. PubMed PMID: 22612266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus. AU - Wang,Tao, AU - Jónsdóttir,Rósa, AU - Liu,Haiyan, AU - Gu,Liwei, AU - Kristinsson,Hordur G, AU - Raghavan,Sivakumar, AU - Olafsdóttir,Gudrún, Y1 - 2012/06/05/ PY - 2012/5/23/entrez PY - 2012/5/23/pubmed PY - 2014/7/19/medline SP - 5874 EP - 83 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 23 N2 - A process for the effective extraction and fractionation of phlorotannins from Fucus vesiculosus with high antioxidant potentials was investigated. The antioxidant activity of F. vesiculosus extract/fractions was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, reducing power, and ferrous ion-chelating assays. Among the crude extract and different polarity fractions, the phlorotannin-enriched ethyl acetate fraction possessed the highest DPPH scavenging activity and reducing power. This fraction was further fractionated by Sephadex LH-20 column chromatography or ultrafiltration. The antioxidant properties were evaluated by both the above chemical antioxidant tests and a mononuclear cell-based bioassay. Sephadex subfractions LH-2 and LH-3 with high total phlorotannin content exhibited strong DPPH quenching activity, comparable to those of ascorbic acid and butylated hydroxytoluene and significantly higher than that of α-tocopherol. Polyphenols in F. vesiculosus were found to consist mainly of high molecular weight phlorotannin polymers. There were no clear relationships between the degree of polymerization, molecular size, and antioxidant activity. All the subfractions separated by Sephadex LH-20 column chromatography and ultrafiltration showed a high ability to scavenge reactive oxygen species generated by mononuclear cells. Further characterization of the phlorotannin compounds was performed on six Sephadex subfractions. Several phlorotannin oligomers were tentatively identified on the basis of HPLC-ESI-MS(n) analyses. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22612266/Antioxidant_capacities_of_phlorotannins_extracted_from_the_brown_algae_Fucus_vesiculosus_ DB - PRIME DP - Unbound Medicine ER -