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Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species.
Dev Comp Immunol 2012; 38(2):262-74DC

Abstract

Ants, as eusocial insects, live in dense groups with high connectivity, increasing the risk of pathogen spread and possibly driving the evolution of their antimicrobial immune system. Draft genomes of seven ant species provide a new source to undertake comparative study of their antimicrobial peptides (AMPs), key components of insect innate immunity. By using computational approaches, we analyzed five AMP families that include abaecins, hymenoptaecins, insect defensins, tachystatins, and crustins in ants, which comprise 69 new members. Among them, a new type of proline-rich abaecins was recognized and they are exclusively present in ants. Hymenoptaecins, a family of glycine-rich AMPs from Hymenoptera and Diptera, exhibit variable numbers of intragenic tandem repeats in a lineage-specific manner and all hymenoptaecins in ants have evolved an acidic C-terminal propeptide. In some ant species, insect defensins with the cysteine-stabilized α-helical and β-sheet (CSαβ) fold and tachystatin-like AMPs with the inhibitor cysteine knot (ICK) fold have undergone gene expansion and differential gene loss. Moreover, extensive sequence diversity exists in the C-termini of the defensins and the ICK-type peptides and the n-loop of the defensins. Also, we identified for the first time a crustin-type AMP in ants, which are only known in crustaceans previously. These ant crustins evolutionarily gain an aromatic amino acid-rich insertion when compared with those of crustaceans. Our work not only enlarges the insect AMP resource, but also sheds light on the complexity and dynamic evolution of AMPs in ants.

Authors+Show Affiliations

Group of Animal Innate Immunity, State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, PR China.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22617650

Citation

Zhang, Zhenting, and Shunyi Zhu. "Comparative Genomics Analysis of Five Families of Antimicrobial Peptide-like Genes in Seven Ant Species." Developmental and Comparative Immunology, vol. 38, no. 2, 2012, pp. 262-74.
Zhang Z, Zhu S. Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species. Dev Comp Immunol. 2012;38(2):262-74.
Zhang, Z., & Zhu, S. (2012). Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species. Developmental and Comparative Immunology, 38(2), pp. 262-74. doi:10.1016/j.dci.2012.05.003.
Zhang Z, Zhu S. Comparative Genomics Analysis of Five Families of Antimicrobial Peptide-like Genes in Seven Ant Species. Dev Comp Immunol. 2012;38(2):262-74. PubMed PMID: 22617650.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative genomics analysis of five families of antimicrobial peptide-like genes in seven ant species. AU - Zhang,Zhenting, AU - Zhu,Shunyi, Y1 - 2012/05/19/ PY - 2012/03/24/received PY - 2012/05/02/revised PY - 2012/05/09/accepted PY - 2012/5/24/entrez PY - 2012/5/24/pubmed PY - 2012/12/12/medline SP - 262 EP - 74 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 38 IS - 2 N2 - Ants, as eusocial insects, live in dense groups with high connectivity, increasing the risk of pathogen spread and possibly driving the evolution of their antimicrobial immune system. Draft genomes of seven ant species provide a new source to undertake comparative study of their antimicrobial peptides (AMPs), key components of insect innate immunity. By using computational approaches, we analyzed five AMP families that include abaecins, hymenoptaecins, insect defensins, tachystatins, and crustins in ants, which comprise 69 new members. Among them, a new type of proline-rich abaecins was recognized and they are exclusively present in ants. Hymenoptaecins, a family of glycine-rich AMPs from Hymenoptera and Diptera, exhibit variable numbers of intragenic tandem repeats in a lineage-specific manner and all hymenoptaecins in ants have evolved an acidic C-terminal propeptide. In some ant species, insect defensins with the cysteine-stabilized α-helical and β-sheet (CSαβ) fold and tachystatin-like AMPs with the inhibitor cysteine knot (ICK) fold have undergone gene expansion and differential gene loss. Moreover, extensive sequence diversity exists in the C-termini of the defensins and the ICK-type peptides and the n-loop of the defensins. Also, we identified for the first time a crustin-type AMP in ants, which are only known in crustaceans previously. These ant crustins evolutionarily gain an aromatic amino acid-rich insertion when compared with those of crustaceans. Our work not only enlarges the insect AMP resource, but also sheds light on the complexity and dynamic evolution of AMPs in ants. SN - 1879-0089 UR - https://www.unboundmedicine.com/medline/citation/22617650/Comparative_genomics_analysis_of_five_families_of_antimicrobial_peptide_like_genes_in_seven_ant_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(12)00090-0 DB - PRIME DP - Unbound Medicine ER -