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Analysis of repaglinide enantiomers in pharmaceutical formulations by capillary electrophoresis using 2,6-di-o-methyl-β-cyclodextrin as a chiral selector.
J Chromatogr Sci. 2012 Sep; 50(8):739-43.JC

Abstract

This study used the general applicability of 2,6-didi-o-methyl-β-cyclodextrin (DM-β-CD) as the chiral selector in capillary electrophoresis for fast and efficient chiral separation of repaglinide enantiomers. A systematic study of the parameters affecting separation was performed with UV detection at 243 nm. The optimum conditions were determined to be 1.25% (w/v) DM-β-CD in 20 mM sodium phosphate (pH 2.5) as the running buffer and separation voltage at 20 kV. DM-β-CD had the best enantiomer resolution properties under the tested conditions, whereas other β-cyclodextrins showed inferior performances or no performance. The proposed method had a linear calibration curve in the concentration range of 12.5-400 µg/mL. The limit of detection was 100 ng/mL. The intra-day and inter-day precisions were 2.8 and 3.2%, respectively. Recoveries of 97.9-100.9% were obtained. The proposed method was fast and convenient, and was determined to be efficient for separating enantiomers and applicable for analyzing repaglinide enantiomers in quality control of pharmaceutical production.

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Authors+Show Affiliations

Li C
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang City, Heibei, People's Republic of China.
Jiang Y
No affiliation info available

MeSH

CarbamatesDrug ContaminationElectrophoresis, CapillaryHydrogen-Ion ConcentrationLimit of DetectionLinear ModelsPiperidinesReproducibility of ResultsStereoisomerismTabletsbeta-Cyclodextrins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22618022

Citation

Li, Cen, and Ye Jiang. "Analysis of Repaglinide Enantiomers in Pharmaceutical Formulations By Capillary Electrophoresis Using 2,6-di-o-methyl-β-cyclodextrin as a Chiral Selector." Journal of Chromatographic Science, vol. 50, no. 8, 2012, pp. 739-43.
Li C, Jiang Y. Analysis of repaglinide enantiomers in pharmaceutical formulations by capillary electrophoresis using 2,6-di-o-methyl-β-cyclodextrin as a chiral selector. J Chromatogr Sci. 2012;50(8):739-43.
Li, C., & Jiang, Y. (2012). Analysis of repaglinide enantiomers in pharmaceutical formulations by capillary electrophoresis using 2,6-di-o-methyl-β-cyclodextrin as a chiral selector. Journal of Chromatographic Science, 50(8), 739-43. https://doi.org/10.1093/chromsci/bms064
Li C, Jiang Y. Analysis of Repaglinide Enantiomers in Pharmaceutical Formulations By Capillary Electrophoresis Using 2,6-di-o-methyl-β-cyclodextrin as a Chiral Selector. J Chromatogr Sci. 2012;50(8):739-43. PubMed PMID: 22618022.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of repaglinide enantiomers in pharmaceutical formulations by capillary electrophoresis using 2,6-di-o-methyl-β-cyclodextrin as a chiral selector. AU - Li,Cen, AU - Jiang,Ye, Y1 - 2012/05/22/ PY - 2012/5/24/entrez PY - 2012/5/24/pubmed PY - 2012/12/12/medline SP - 739 EP - 43 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 50 IS - 8 N2 - This study used the general applicability of 2,6-didi-o-methyl-β-cyclodextrin (DM-β-CD) as the chiral selector in capillary electrophoresis for fast and efficient chiral separation of repaglinide enantiomers. A systematic study of the parameters affecting separation was performed with UV detection at 243 nm. The optimum conditions were determined to be 1.25% (w/v) DM-β-CD in 20 mM sodium phosphate (pH 2.5) as the running buffer and separation voltage at 20 kV. DM-β-CD had the best enantiomer resolution properties under the tested conditions, whereas other β-cyclodextrins showed inferior performances or no performance. The proposed method had a linear calibration curve in the concentration range of 12.5-400 µg/mL. The limit of detection was 100 ng/mL. The intra-day and inter-day precisions were 2.8 and 3.2%, respectively. Recoveries of 97.9-100.9% were obtained. The proposed method was fast and convenient, and was determined to be efficient for separating enantiomers and applicable for analyzing repaglinide enantiomers in quality control of pharmaceutical production. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/22618022/Analysis_of_repaglinide_enantiomers_in_pharmaceutical_formulations_by_capillary_electrophoresis_using_26_di_o_methyl_β_cyclodextrin_as_a_chiral_selector_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bms064 DB - PRIME DP - Unbound Medicine ER -