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Curcumin protects nigral dopaminergic neurons by iron-chelation in the 6-hydroxydopamine rat model of Parkinson's disease.
Neurosci Bull. 2012 Jun; 28(3):253-8.NB

Abstract

OBJECTIVE

Curcumin is a plant polyphenolic compound and a major component of spice turmeric (Curcuma longa). It has been reported to possess free radical-scavenging, iron-chelating, and anti-inflammatory properties in different tissues. Our previous study showed that curcumin protects MES23.5 dopaminergic cells from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro. The present study aimed to explore this neuroprotective effect in the 6-OHDA-lesioned rat model of Parkinson's disease in vivo.

METHODS

Rats were given intragastric curcumin for 24 days. 6-OHDA lesioning was conducted on day 4 of curcumin treatment. Dopamine content was assessed by high-performance liquid chromatography with electrochemical detection, tyrosine hydroxylase (TH)-containing neurons by immunohistochemistry, and iron-containing cells by Perls' iron staining.

RESULTS

The dopamine content in the striatum and the number of TH-immunoreactive neurons decreased after 6-OHDA treatment. Curcumin pretreatment reversed these changes. Further studies demonstrated that 6-OHDA treatment increased the number of iron-staining cells, which was dramatically decreased by curcumin pretreatment.

CONCLUSION

The protective effects of curcumin against 6-OHDA may be attributable to the iron-chelating activity of curcumin to suppress the iron-induced degeneration of nigral dopaminergic neurons.

Links

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Authors+Show Affiliations

Du XX
Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Dis-orders and State Key Disciplines: Physiology, Medical College of Qingdao University, Qingdao 266071, China.
Xu HM
No affiliation info available
Jiang H
No affiliation info available
Song N
No affiliation info available
Wang J
No affiliation info available
Xie JX
No affiliation info available

MeSH

AnimalsCurcuminDisease Models, AnimalDopaminergic NeuronsFemaleIronIron Chelating AgentsNeuroprotective AgentsOxidopamineParkinson Disease, SecondaryRatsRats, WistarSubstantia Nigra

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22622825

Citation

Du, Xi-Xun, et al. "Curcumin Protects Nigral Dopaminergic Neurons By Iron-chelation in the 6-hydroxydopamine Rat Model of Parkinson's Disease." Neuroscience Bulletin, vol. 28, no. 3, 2012, pp. 253-8.
Du XX, Xu HM, Jiang H, et al. Curcumin protects nigral dopaminergic neurons by iron-chelation in the 6-hydroxydopamine rat model of Parkinson's disease. Neurosci Bull. 2012;28(3):253-8.
Du, X. X., Xu, H. M., Jiang, H., Song, N., Wang, J., & Xie, J. X. (2012). Curcumin protects nigral dopaminergic neurons by iron-chelation in the 6-hydroxydopamine rat model of Parkinson's disease. Neuroscience Bulletin, 28(3), 253-8. https://doi.org/10.1007/s12264-012-1238-2
Du XX, et al. Curcumin Protects Nigral Dopaminergic Neurons By Iron-chelation in the 6-hydroxydopamine Rat Model of Parkinson's Disease. Neurosci Bull. 2012;28(3):253-8. PubMed PMID: 22622825.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin protects nigral dopaminergic neurons by iron-chelation in the 6-hydroxydopamine rat model of Parkinson's disease. AU - Du,Xi-Xun, AU - Xu,Hua-Min, AU - Jiang,Hong, AU - Song,Ning, AU - Wang,Jun, AU - Xie,Jun-Xia, PY - 2012/5/25/entrez PY - 2012/5/25/pubmed PY - 2014/4/22/medline SP - 253 EP - 8 JF - Neuroscience bulletin JO - Neurosci Bull VL - 28 IS - 3 N2 - OBJECTIVE: Curcumin is a plant polyphenolic compound and a major component of spice turmeric (Curcuma longa). It has been reported to possess free radical-scavenging, iron-chelating, and anti-inflammatory properties in different tissues. Our previous study showed that curcumin protects MES23.5 dopaminergic cells from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in vitro. The present study aimed to explore this neuroprotective effect in the 6-OHDA-lesioned rat model of Parkinson's disease in vivo. METHODS: Rats were given intragastric curcumin for 24 days. 6-OHDA lesioning was conducted on day 4 of curcumin treatment. Dopamine content was assessed by high-performance liquid chromatography with electrochemical detection, tyrosine hydroxylase (TH)-containing neurons by immunohistochemistry, and iron-containing cells by Perls' iron staining. RESULTS: The dopamine content in the striatum and the number of TH-immunoreactive neurons decreased after 6-OHDA treatment. Curcumin pretreatment reversed these changes. Further studies demonstrated that 6-OHDA treatment increased the number of iron-staining cells, which was dramatically decreased by curcumin pretreatment. CONCLUSION: The protective effects of curcumin against 6-OHDA may be attributable to the iron-chelating activity of curcumin to suppress the iron-induced degeneration of nigral dopaminergic neurons. SN - 1995-8218 UR - https://www.unboundmedicine.com/medline/citation/22622825/Curcumin_protects_nigral_dopaminergic_neurons_by_iron_chelation_in_the_6_hydroxydopamine_rat_model_of_Parkinson's_disease_ DB - PRIME DP - Unbound Medicine ER -