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Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum).
Genome. 2012 Jun; 55(6):417-27.G

Abstract

The aims of the present study were to provide deletion maps for wheat (Triticum aestivum L.) chromosomes 5A and 5B and a detailed genetic map of chromosome 5A enriched with popular microsatellite markers, which could be compared with other existing maps and useful for mapping major genes and quantitative traits loci (QTL). Physical mapping of 165 gSSR and EST-SSR markers was conducted by amplifying each primer pair on Chinese Spring, aneuploid lines, and deletion lines for the homoeologous group 5 chromosomes. A recombinant inbred line (RIL) mapping population that is recombinant for only chromosome 5A was obtained by crossing the wheat cultivar Chinese Spring and the disomic substitution line Chinese Spring-5A dicoccoides and was used to develop a genetic linkage map of chromosome 5A. A total of 67 markers were found polymorphic between the parental lines and were mapped in the RIL population. Sixty-three loci and the Q gene were clustered in three linkage groups ordered at a minimum LOD score of 5, while four loci remained unlinked. The whole genetic 5A chromosome map covered 420.2 cM, distributed among three linkage groups of 189.3, 35.4, and 195.5 cM. The EST sequences located on chromosomes 5A and 5B were used for comparative analysis against Brachypodium distachyon (L.) P. Beauv. and rice (Oryza sativa L.) genomes to resolve orthologous relationships among the genomes of wheat and the two model species.

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Authors+Show Affiliations

Gadaleta A
Department of Agro-Forestry and Environmental Biology and Chemistry, Section of Genetics and Plant Breeding, University of Bari Aldo Moro, Via Amendola 165/A, 70126 - Bari, Italy. agata.gadaleta@agr.uniba.it
Giancaspro A
No affiliation info available
Giove SL
No affiliation info available
Zacheo S
No affiliation info available
Incerti O
No affiliation info available
Simeone R
No affiliation info available
Colasuonno P
No affiliation info available
Nigro D
No affiliation info available
Valè G
No affiliation info available
Cattivelli L
No affiliation info available
Stanca M
No affiliation info available
Blanco A
No affiliation info available

MeSH

Base SequenceBrachypodiumChromosome MappingChromosomes, PlantDNA PrimersExpressed Sequence TagsGenes, PlantGenetic LinkageGenome, PlantMicrosatellite RepeatsMultigene FamilyOryzaQuantitative Trait LociSequence DeletionTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22624876

Citation

Gadaleta, A, et al. "Development of a Deletion and Genetic Linkage Map for the 5A and 5B Chromosomes of Wheat (Triticum Aestivum)." Genome, vol. 55, no. 6, 2012, pp. 417-27.
Gadaleta A, Giancaspro A, Giove SL, et al. Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum). Genome. 2012;55(6):417-27.
Gadaleta, A., Giancaspro, A., Giove, S. L., Zacheo, S., Incerti, O., Simeone, R., Colasuonno, P., Nigro, D., Valè, G., Cattivelli, L., Stanca, M., & Blanco, A. (2012). Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum). Genome, 55(6), 417-27. https://doi.org/10.1139/g2012-028
Gadaleta A, et al. Development of a Deletion and Genetic Linkage Map for the 5A and 5B Chromosomes of Wheat (Triticum Aestivum). Genome. 2012;55(6):417-27. PubMed PMID: 22624876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a deletion and genetic linkage map for the 5A and 5B chromosomes of wheat (Triticum aestivum). AU - Gadaleta,A, AU - Giancaspro,A, AU - Giove,S L, AU - Zacheo,S, AU - Incerti,O, AU - Simeone,R, AU - Colasuonno,P, AU - Nigro,D, AU - Valè,G, AU - Cattivelli,L, AU - Stanca,M, AU - Blanco,A, Y1 - 2012/05/25/ PY - 2012/5/26/entrez PY - 2012/5/26/pubmed PY - 2012/10/2/medline SP - 417 EP - 27 JF - Genome JO - Genome VL - 55 IS - 6 N2 - The aims of the present study were to provide deletion maps for wheat (Triticum aestivum L.) chromosomes 5A and 5B and a detailed genetic map of chromosome 5A enriched with popular microsatellite markers, which could be compared with other existing maps and useful for mapping major genes and quantitative traits loci (QTL). Physical mapping of 165 gSSR and EST-SSR markers was conducted by amplifying each primer pair on Chinese Spring, aneuploid lines, and deletion lines for the homoeologous group 5 chromosomes. A recombinant inbred line (RIL) mapping population that is recombinant for only chromosome 5A was obtained by crossing the wheat cultivar Chinese Spring and the disomic substitution line Chinese Spring-5A dicoccoides and was used to develop a genetic linkage map of chromosome 5A. A total of 67 markers were found polymorphic between the parental lines and were mapped in the RIL population. Sixty-three loci and the Q gene were clustered in three linkage groups ordered at a minimum LOD score of 5, while four loci remained unlinked. The whole genetic 5A chromosome map covered 420.2 cM, distributed among three linkage groups of 189.3, 35.4, and 195.5 cM. The EST sequences located on chromosomes 5A and 5B were used for comparative analysis against Brachypodium distachyon (L.) P. Beauv. and rice (Oryza sativa L.) genomes to resolve orthologous relationships among the genomes of wheat and the two model species. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/22624876/Development_of_a_deletion_and_genetic_linkage_map_for_the_5A_and_5B_chromosomes_of_wheat__Triticum_aestivum__ L2 - https://cdnsciencepub.com/doi/10.1139/g2012-028?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -