Tags

Type your tag names separated by a space and hit enter

Autotransplanting of bone marrow-derived mononuclear cells for complete cases of canine paraplegia and loss of pain perception, secondary to intervertebral disc herniation.
Exp Clin Transplant. 2012 Jun; 10(3):263-72.EC

Abstract

OBJECTIVES

Severe intervertebral disc herniation causes complete paraplegia and loss of pain sensation in canines. The prognosis is poor, even when decompression surgery is performed immediately after onset. Studies suggest that bone marrow-derived mononuclear cells will regenerate the injured spinal cord and restore neurologic function. This study was conducted to assess the clinical efficacy of bone marrow-derived mononuclear cell autotransplanting in severe cases of canine intervertebral disc herniation.

MATERIALS AND METHODS

Eighty-two dogs (miniature dachshunds) with severe thoracolumbar intervertebral disc herniation were used. All had intervertebral disc herniation accompanied by paraplegia and loss of pain perception. In 36 dogs, bone marrow-derived mononuclear cells were autotransplanted to the lesioned spinal cord immediately after decompression surgery. Bone marrow was collected from the proximal humerus and subjected to density gradient centrifugation to isolate the bone marrow-derived mononuclear cells. The remaining 46 dogs (receiving surgical treatment only) were assigned as controls. Therapeutic efficacy was compared based on the rate of ambulatory recovery.

RESULTS

Ambulatory recovery was observed in 88.9% and 56.5% of animals in the bone marrow-derived mononuclear cells and control groups, and a significant difference was found. No complications were found in bone marrow-derived mononuclear cells group.

CONCLUSIONS

Bone marrow-derived mononuclear cell transplanting revealed a significant increase in the recovery rate and, as has been reported in rats and humans, bone marrow-derived mononuclear cell autotransplanting shows efficacy in canines as well.

Authors+Show Affiliations

Division of Veterinary Surgery, Nippon Veterinary and Life Science University, and Aikouishida Animal Hospital, Tokyo, Japan. katsutoshi@vet.nameNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22631064

Citation

Tamura, Katsutoshi, et al. "Autotransplanting of Bone Marrow-derived Mononuclear Cells for Complete Cases of Canine Paraplegia and Loss of Pain Perception, Secondary to Intervertebral Disc Herniation." Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation, vol. 10, no. 3, 2012, pp. 263-72.
Tamura K, Harada Y, Nagashima N, et al. Autotransplanting of bone marrow-derived mononuclear cells for complete cases of canine paraplegia and loss of pain perception, secondary to intervertebral disc herniation. Exp Clin Transplant. 2012;10(3):263-72.
Tamura, K., Harada, Y., Nagashima, N., Itoi, T., Ishino, H., Yogo, T., Nezu, Y., Hara, Y., Suzuki, Y., Ide, C., & Tagawa, M. (2012). Autotransplanting of bone marrow-derived mononuclear cells for complete cases of canine paraplegia and loss of pain perception, secondary to intervertebral disc herniation. Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation, 10(3), 263-72.
Tamura K, et al. Autotransplanting of Bone Marrow-derived Mononuclear Cells for Complete Cases of Canine Paraplegia and Loss of Pain Perception, Secondary to Intervertebral Disc Herniation. Exp Clin Transplant. 2012;10(3):263-72. PubMed PMID: 22631064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autotransplanting of bone marrow-derived mononuclear cells for complete cases of canine paraplegia and loss of pain perception, secondary to intervertebral disc herniation. AU - Tamura,Katsutoshi, AU - Harada,Yasuji, AU - Nagashima,Naho, AU - Itoi,Takamasa, AU - Ishino,Hirokazu, AU - Yogo,Takuya, AU - Nezu,Yoshinori, AU - Hara,Yasushi, AU - Suzuki,Yoshihisa, AU - Ide,Chizuka, AU - Tagawa,Masahiro, PY - 2012/5/29/entrez PY - 2012/5/29/pubmed PY - 2013/1/3/medline SP - 263 EP - 72 JF - Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation JO - Exp Clin Transplant VL - 10 IS - 3 N2 - OBJECTIVES: Severe intervertebral disc herniation causes complete paraplegia and loss of pain sensation in canines. The prognosis is poor, even when decompression surgery is performed immediately after onset. Studies suggest that bone marrow-derived mononuclear cells will regenerate the injured spinal cord and restore neurologic function. This study was conducted to assess the clinical efficacy of bone marrow-derived mononuclear cell autotransplanting in severe cases of canine intervertebral disc herniation. MATERIALS AND METHODS: Eighty-two dogs (miniature dachshunds) with severe thoracolumbar intervertebral disc herniation were used. All had intervertebral disc herniation accompanied by paraplegia and loss of pain perception. In 36 dogs, bone marrow-derived mononuclear cells were autotransplanted to the lesioned spinal cord immediately after decompression surgery. Bone marrow was collected from the proximal humerus and subjected to density gradient centrifugation to isolate the bone marrow-derived mononuclear cells. The remaining 46 dogs (receiving surgical treatment only) were assigned as controls. Therapeutic efficacy was compared based on the rate of ambulatory recovery. RESULTS: Ambulatory recovery was observed in 88.9% and 56.5% of animals in the bone marrow-derived mononuclear cells and control groups, and a significant difference was found. No complications were found in bone marrow-derived mononuclear cells group. CONCLUSIONS: Bone marrow-derived mononuclear cell transplanting revealed a significant increase in the recovery rate and, as has been reported in rats and humans, bone marrow-derived mononuclear cell autotransplanting shows efficacy in canines as well. SN - 2146-8427 UR - https://www.unboundmedicine.com/medline/citation/22631064/Autotransplanting_of_bone_marrow_derived_mononuclear_cells_for_complete_cases_of_canine_paraplegia_and_loss_of_pain_perception_secondary_to_intervertebral_disc_herniation_ L2 - http://www.ectrx.org/forms/ectrxcontentshow.php?year=2012&volume=10&issue=3&supplement=0&makale_no=0&spage_number=263&content_type=FULL TEXT DB - PRIME DP - Unbound Medicine ER -