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Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model.
Stem Cells. 2012 Aug; 30(8):1675-84.SC

Abstract

Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn(F7/F7) mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies.

Authors+Show Affiliations

Department of Pediatrics and Pediatric Surgery, University of Padova, Padova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22644669

Citation

Piccoli, Martina, et al. "Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA-Cre, Smn(F7/F7) Mouse Model." Stem Cells (Dayton, Ohio), vol. 30, no. 8, 2012, pp. 1675-84.
Piccoli M, Franzin C, Bertin E, et al. Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model. Stem Cells. 2012;30(8):1675-84.
Piccoli, M., Franzin, C., Bertin, E., Urbani, L., Blaauw, B., Repele, A., Taschin, E., Cenedese, A., Zanon, G. F., André-Schmutz, I., Rosato, A., Melki, J., Cavazzana-Calvo, M., Pozzobon, M., & De Coppi, P. (2012). Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model. Stem Cells (Dayton, Ohio), 30(8), 1675-84. https://doi.org/10.1002/stem.1134
Piccoli M, et al. Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA-Cre, Smn(F7/F7) Mouse Model. Stem Cells. 2012;30(8):1675-84. PubMed PMID: 22644669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amniotic fluid stem cells restore the muscle cell niche in a HSA-Cre, Smn(F7/F7) mouse model. AU - Piccoli,Martina, AU - Franzin,Chiara, AU - Bertin,Enrica, AU - Urbani,Luca, AU - Blaauw,Bert, AU - Repele,Andrea, AU - Taschin,Elisa, AU - Cenedese,Angelo, AU - Zanon,Giovanni Franco, AU - André-Schmutz,Isabelle, AU - Rosato,Antonio, AU - Melki,Judith, AU - Cavazzana-Calvo,Marina, AU - Pozzobon,Michela, AU - De Coppi,Paolo, PY - 2012/5/31/entrez PY - 2012/5/31/pubmed PY - 2013/3/1/medline SP - 1675 EP - 84 JF - Stem cells (Dayton, Ohio) JO - Stem Cells VL - 30 IS - 8 N2 - Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn(F7/F7) mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies. SN - 1549-4918 UR - https://www.unboundmedicine.com/medline/citation/22644669/Amniotic_fluid_stem_cells_restore_the_muscle_cell_niche_in_a_HSA_Cre_Smn_F7/F7__mouse_model_ L2 - https://doi.org/10.1002/stem.1134 DB - PRIME DP - Unbound Medicine ER -