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Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding.
BMC Plant Biol. 2012 May 30; 12:73.BP

Abstract

BACKGROUND

High molecular weight glutenin subunits (HMW-GSs), encoded by the genes at Glu-1 loci in wheat and its related species, are significant in the determination of grain processing quality. However, the diversity and variations of HMW-GSs are relatively low in bread wheat. More interests are now focused on wheat wild relatives in Triticeae. The genus Aegilops represents an important germplasm for novel HWM-GSs and other useful genes for wheat genetic improvement.

RESULTS

Six novel Glu-1 alleles and HMW-GSs were identified and characterized from three species of Aegilops section Sitopsis (S genome). Both open reading frames (ORFs) and promoter regions of these Glu-1 alleles were sequenced and characterized. The ORFs of Sitopsis Glu-1 genes are approximately 2.9 kb and 2.3 kb for x-type and y-type subunits, respectively. Although the primary structures of Sitopsis HMW-GSs are similar to those of previously reported ones, all six x-type or y-type subunits have the large fragment insertions. Our comparative analyses of the deduced amino acid sequences verified that Aegilops section Sitopsis species encode novel HMW-GSs with their molecular weights larger than almost all other known HMW-GSs. The Glu-1 promoter sequences share the high homology among S genome. Our phylogenetic analyses by both network and NJ tree indicated that there is a close phylogenetic evolutionary relationship of x-type and y-type subunit between S and D genome.

CONCLUSIONS

The large molecular weight of HMW-GSs from S genome is a unique feature identified in this study. Such large subunits are resulted from the duplications of repetitive domains in Sitopsis HMW-GSs. The unequal crossover events are the most likely mechanism of variations in glutenin subunits. The S genome-encoded subunits, 1Dx2.2 and 1Dx2.2* have independent origins, although they share similar evolutionary mechanism. As HMW-GSs play a key role in wheat baking quality, these large Sitopsis glutenin subunits can be used as special genetic resources for wheat quality improvement.

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22646663

Citation

Jiang, Qian-Tao, et al. "Novel Variants of HMW Glutenin Subunits From Aegilops Section Sitopsis Species in Relation to Evolution and Wheat Breeding." BMC Plant Biology, vol. 12, 2012, p. 73.
Jiang QT, Ma J, Wei YM, et al. Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding. BMC Plant Biol. 2012;12:73.
Jiang, Q. T., Ma, J., Wei, Y. M., Liu, Y. X., Lan, X. J., Dai, S. F., Lu, Z. X., Zhao, S., Zhao, Q. Z., & Zheng, Y. L. (2012). Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding. BMC Plant Biology, 12, 73. https://doi.org/10.1186/1471-2229-12-73
Jiang QT, et al. Novel Variants of HMW Glutenin Subunits From Aegilops Section Sitopsis Species in Relation to Evolution and Wheat Breeding. BMC Plant Biol. 2012 May 30;12:73. PubMed PMID: 22646663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel variants of HMW glutenin subunits from Aegilops section Sitopsis species in relation to evolution and wheat breeding. AU - Jiang,Qian-Tao, AU - Ma,Jian, AU - Wei,Yu-Ming, AU - Liu,Ya-Xi, AU - Lan,Xiu-Jin, AU - Dai,Shou-Fen, AU - Lu,Zhen-Xiang, AU - Zhao,Shan, AU - Zhao,Quan-Zhi, AU - Zheng,You-Liang, Y1 - 2012/05/30/ PY - 2011/09/19/received PY - 2012/05/11/accepted PY - 2012/6/1/entrez PY - 2012/6/1/pubmed PY - 2014/8/27/medline SP - 73 EP - 73 JF - BMC plant biology JO - BMC Plant Biol VL - 12 N2 - BACKGROUND: High molecular weight glutenin subunits (HMW-GSs), encoded by the genes at Glu-1 loci in wheat and its related species, are significant in the determination of grain processing quality. However, the diversity and variations of HMW-GSs are relatively low in bread wheat. More interests are now focused on wheat wild relatives in Triticeae. The genus Aegilops represents an important germplasm for novel HWM-GSs and other useful genes for wheat genetic improvement. RESULTS: Six novel Glu-1 alleles and HMW-GSs were identified and characterized from three species of Aegilops section Sitopsis (S genome). Both open reading frames (ORFs) and promoter regions of these Glu-1 alleles were sequenced and characterized. The ORFs of Sitopsis Glu-1 genes are approximately 2.9 kb and 2.3 kb for x-type and y-type subunits, respectively. Although the primary structures of Sitopsis HMW-GSs are similar to those of previously reported ones, all six x-type or y-type subunits have the large fragment insertions. Our comparative analyses of the deduced amino acid sequences verified that Aegilops section Sitopsis species encode novel HMW-GSs with their molecular weights larger than almost all other known HMW-GSs. The Glu-1 promoter sequences share the high homology among S genome. Our phylogenetic analyses by both network and NJ tree indicated that there is a close phylogenetic evolutionary relationship of x-type and y-type subunit between S and D genome. CONCLUSIONS: The large molecular weight of HMW-GSs from S genome is a unique feature identified in this study. Such large subunits are resulted from the duplications of repetitive domains in Sitopsis HMW-GSs. The unequal crossover events are the most likely mechanism of variations in glutenin subunits. The S genome-encoded subunits, 1Dx2.2 and 1Dx2.2* have independent origins, although they share similar evolutionary mechanism. As HMW-GSs play a key role in wheat baking quality, these large Sitopsis glutenin subunits can be used as special genetic resources for wheat quality improvement. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/22646663/Novel_variants_of_HMW_glutenin_subunits_from_Aegilops_section_Sitopsis_species_in_relation_to_evolution_and_wheat_breeding_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-12-73 DB - PRIME DP - Unbound Medicine ER -