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OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity.
J Eur Acad Dermatol Venereol 2013; 27(2):e197-205JE

Abstract

BACKGROUND

The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD).

OBJECTIVE

The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin.

METHODS

OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non-lesional skin of 17 subjects with moderate-to-severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA-stimulated keratinocytes or LAD-2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM-1.

RESULTS

We show for the first time that the staining intensity of OX40L and the number of OX40(+) cells are significantly greater in the lesional dermis than in the healthy-looking dermis in AD (P < 0.001 in both comparisons) and also in psoriasis (P = 0.01 and P < 0.001 respectively), but neither molecule correlate significantly with the clinical severity of AD. Living keratinocytes and cell membranes from LAD-2 mast cells and keratinocytes increased the PBMC proliferation response. Anti-OX40L antibody inhibited, in a similar fashion as anti-ICAM-1 and anti-CD30L, PBMC proliferation induced by LAD-2 membranes, but stimulated that induced by keratinocytes.

CONCLUSION

Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction.

Authors+Show Affiliations

Department of Dermatology, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland. tiina.ilves@kuh.fiNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22646697

Citation

Ilves, T, and I T. Harvima. "OX40 Ligand and OX40 Are Increased in Atopic Dermatitis Lesions but Do Not Correlate With Clinical Severity." Journal of the European Academy of Dermatology and Venereology : JEADV, vol. 27, no. 2, 2013, pp. e197-205.
Ilves T, Harvima IT. OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity. J Eur Acad Dermatol Venereol. 2013;27(2):e197-205.
Ilves, T., & Harvima, I. T. (2013). OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity. Journal of the European Academy of Dermatology and Venereology : JEADV, 27(2), pp. e197-205. doi:10.1111/j.1468-3083.2012.04587.x.
Ilves T, Harvima IT. OX40 Ligand and OX40 Are Increased in Atopic Dermatitis Lesions but Do Not Correlate With Clinical Severity. J Eur Acad Dermatol Venereol. 2013;27(2):e197-205. PubMed PMID: 22646697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OX40 ligand and OX40 are increased in atopic dermatitis lesions but do not correlate with clinical severity. AU - Ilves,T, AU - Harvima,I T, Y1 - 2012/05/31/ PY - 2012/6/1/entrez PY - 2012/6/1/pubmed PY - 2013/7/3/medline SP - e197 EP - 205 JF - Journal of the European Academy of Dermatology and Venereology : JEADV JO - J Eur Acad Dermatol Venereol VL - 27 IS - 2 N2 - BACKGROUND: The interaction between the OX40 ligand (OX40L) and OX40 has been suggested to have pathogenetic significance in atopic dermatitis (AD). OBJECTIVE: The purpose of this study was to investigate the expression and relevance of OX40L and OX40 in AD skin. METHODS: OX40L and OX40 were stained immunohistochemically on the cryosections of the lesional and non-lesional skin of 17 subjects with moderate-to-severe AD and of 10 patients with psoriasis vulgaris. Phorbol myristate acetate (PMA) stimulated keratinocytes and cell membrane preparations from PMA-stimulated keratinocytes or LAD-2 mast cells were incubated with peripheral blood mononuclear cells (PBMC) in the presence or absence of blocking monoclonal antibodies to OX40L, CD30L or ICAM-1. RESULTS: We show for the first time that the staining intensity of OX40L and the number of OX40(+) cells are significantly greater in the lesional dermis than in the healthy-looking dermis in AD (P < 0.001 in both comparisons) and also in psoriasis (P = 0.01 and P < 0.001 respectively), but neither molecule correlate significantly with the clinical severity of AD. Living keratinocytes and cell membranes from LAD-2 mast cells and keratinocytes increased the PBMC proliferation response. Anti-OX40L antibody inhibited, in a similar fashion as anti-ICAM-1 and anti-CD30L, PBMC proliferation induced by LAD-2 membranes, but stimulated that induced by keratinocytes. CONCLUSION: Our findings provide evidence for the involvement of OX40 and OX40L in the pathogenesis of AD though they are not specific to AD and in vitro results suggest complex interaction. SN - 1468-3083 UR - https://www.unboundmedicine.com/medline/citation/22646697/OX40_ligand_and_OX40_are_increased_in_atopic_dermatitis_lesions_but_do_not_correlate_with_clinical_severity_ L2 - https://doi.org/10.1111/j.1468-3083.2012.04587.x DB - PRIME DP - Unbound Medicine ER -