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DNA damage and biochemical toxicity of antibiotics in soil on the earthworm Eisenia fetida.
Chemosphere. 2012 Sep; 89(1):44-51.C

Abstract

DNA damage and changes in enzyme activities were used as biomarkers to evaluate the genotoxicity and oxidative stress of tetracycline and chlortetracycline on the earthworm Eisenia fetida. The results showed that both antibiotics induced significant genotoxicity on earthworms in a dose-dependent manner (p<0.01) with chlortetracycline having a stronger effect than tetracycline in the short term. The tests on the activities of superoxide dismutase (SOD) and catalase (CAT) enzymes further indicated biochemical stresses induced by the antibiotics. An N-shaped activity pattern was noted with the enzyme activities being stimulated first, then inhibited, and stimulated again with increasing concentration. The induced activity of SOD or CAT could scavenge oxygen free radicals and protect the organisms against oxidative stress by alleviating the corresponding DNA damage. Compared to enzyme activities, DNA damage as a biomarker was more sensitive and is thus more suitable for detecting low concentration exposure and diagnosing the genotoxicity of contaminants in terrestrial environment.

Authors+Show Affiliations

Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22647195

Citation

Dong, Luxi, et al. "DNA Damage and Biochemical Toxicity of Antibiotics in Soil On the Earthworm Eisenia Fetida." Chemosphere, vol. 89, no. 1, 2012, pp. 44-51.
Dong L, Gao J, Xie X, et al. DNA damage and biochemical toxicity of antibiotics in soil on the earthworm Eisenia fetida. Chemosphere. 2012;89(1):44-51.
Dong, L., Gao, J., Xie, X., & Zhou, Q. (2012). DNA damage and biochemical toxicity of antibiotics in soil on the earthworm Eisenia fetida. Chemosphere, 89(1), 44-51. https://doi.org/10.1016/j.chemosphere.2012.04.010
Dong L, et al. DNA Damage and Biochemical Toxicity of Antibiotics in Soil On the Earthworm Eisenia Fetida. Chemosphere. 2012;89(1):44-51. PubMed PMID: 22647195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA damage and biochemical toxicity of antibiotics in soil on the earthworm Eisenia fetida. AU - Dong,Luxi, AU - Gao,Jie, AU - Xie,Xiujie, AU - Zhou,Qixing, Y1 - 2012/05/28/ PY - 2011/10/18/received PY - 2012/03/19/revised PY - 2012/04/04/accepted PY - 2012/6/1/entrez PY - 2012/6/1/pubmed PY - 2012/11/14/medline SP - 44 EP - 51 JF - Chemosphere JO - Chemosphere VL - 89 IS - 1 N2 - DNA damage and changes in enzyme activities were used as biomarkers to evaluate the genotoxicity and oxidative stress of tetracycline and chlortetracycline on the earthworm Eisenia fetida. The results showed that both antibiotics induced significant genotoxicity on earthworms in a dose-dependent manner (p<0.01) with chlortetracycline having a stronger effect than tetracycline in the short term. The tests on the activities of superoxide dismutase (SOD) and catalase (CAT) enzymes further indicated biochemical stresses induced by the antibiotics. An N-shaped activity pattern was noted with the enzyme activities being stimulated first, then inhibited, and stimulated again with increasing concentration. The induced activity of SOD or CAT could scavenge oxygen free radicals and protect the organisms against oxidative stress by alleviating the corresponding DNA damage. Compared to enzyme activities, DNA damage as a biomarker was more sensitive and is thus more suitable for detecting low concentration exposure and diagnosing the genotoxicity of contaminants in terrestrial environment. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/22647195/DNA_damage_and_biochemical_toxicity_of_antibiotics_in_soil_on_the_earthworm_Eisenia_fetida_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(12)00497-3 DB - PRIME DP - Unbound Medicine ER -