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[Effects of electroacupuncture of "Shuanggu Yitong" prescription on the T lymphocyte subset proportions in aging rats].
Zhongguo Zhen Jiu. 2012 May; 32(5):435-9.ZZ

Abstract

OBJECTIVE

To explore the mechanism of electroacupuncture of "Shuanggu Yitong" prescription on postponing aging.

METHODS

Forty 3-month SD rats, male only, 30 rats were made sub-acute aging model by D-galactose s.c. injection continuously for 42 d, and rest of the rats, 10, were divided into a normal control group. After the modeling, the sub-acute aging model rats were randomly into a Shuanggu Yitong group [electroacupuncture at "Guanyuan" (CV 4) and "Zusanli" (ST 36), hand needle at "Baihui" (GV 20)], an acupuncture control group [electroacupuncture at "Weizhong" (BL 40) and "Shuifen" (CV 9), hand needle at "Yintang" (GV 29)] and an aging model group, ten in each one. The treatment was given once in a day, six of which made a course. The rats in the normal control group and aging model group were not received any treatment. After the treatment for three weeks, the rats were put to death and their spleen index, thymus index and the T lymphocytes subgroups (CD8(+) T/T cell and CD8(+) CD28(-) T/CD8(+) T cell) were tested.

RESULTS

The spleen index (1.74 +/- 0.059) and thymus index (0.64 +/- 0.039) in the aging model group was obviously lower than those in the normal control group (1.93 +/- 0.061), (0.81 +/- 0.053) respectively (both P < 0.05); the CD8(+) CD28(-) T/CD8(+) T cell percentages (26.28 +/- 4.69)% and CD8(+) T/T cell percentages (43.33 +/- 2.84)% in the aging model group were both significantly higher than those (15.08 +/- 5.58)% (P < 0.01), (34.70 +/- 4.24)% (P < 0.01) in the normal control group. Compared with the aging model group, the spleen index (1.91 +/- 0.081) and thymus index (0.79 +/- 0.080) in the Shuanggu Yitong group were significantly higher (both P < 0.05), but obviously decreased with the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73) (P < 0.01); the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73)% in the acupuncture control group was also lower than the aging model group (P < 0.05), but more obvious reduce for the Shuanggu Yitong group (P < 0.05).

CONCLUSION

The treatment of Shuanggu Yitong prescription could regulate the proportions of the T lymphocyte subset, and slow down the immunosenescence of subacute aging model rats induced by D-galactose.

Authors+Show Affiliations

College of Acupuncture and Orthopedics, Hubei University of CM, Wuhan 430061, Hubei Province, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22650133

Citation

Xiao, Ling, et al. "[Effects of Electroacupuncture of "Shuanggu Yitong" Prescription On the T Lymphocyte Subset Proportions in Aging Rats]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 32, no. 5, 2012, pp. 435-9.
Xiao L, Wang GA, Wang H. [Effects of electroacupuncture of "Shuanggu Yitong" prescription on the T lymphocyte subset proportions in aging rats]. Zhongguo Zhen Jiu. 2012;32(5):435-9.
Xiao, L., Wang, G. A., & Wang, H. (2012). [Effects of electroacupuncture of "Shuanggu Yitong" prescription on the T lymphocyte subset proportions in aging rats]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 32(5), 435-9.
Xiao L, Wang GA, Wang H. [Effects of Electroacupuncture of "Shuanggu Yitong" Prescription On the T Lymphocyte Subset Proportions in Aging Rats]. Zhongguo Zhen Jiu. 2012;32(5):435-9. PubMed PMID: 22650133.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture of "Shuanggu Yitong" prescription on the T lymphocyte subset proportions in aging rats]. AU - Xiao,Ling, AU - Wang,Guang-An, AU - Wang,Hua, PY - 2012/6/2/entrez PY - 2012/6/2/pubmed PY - 2012/7/18/medline SP - 435 EP - 9 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 32 IS - 5 N2 - OBJECTIVE: To explore the mechanism of electroacupuncture of "Shuanggu Yitong" prescription on postponing aging. METHODS: Forty 3-month SD rats, male only, 30 rats were made sub-acute aging model by D-galactose s.c. injection continuously for 42 d, and rest of the rats, 10, were divided into a normal control group. After the modeling, the sub-acute aging model rats were randomly into a Shuanggu Yitong group [electroacupuncture at "Guanyuan" (CV 4) and "Zusanli" (ST 36), hand needle at "Baihui" (GV 20)], an acupuncture control group [electroacupuncture at "Weizhong" (BL 40) and "Shuifen" (CV 9), hand needle at "Yintang" (GV 29)] and an aging model group, ten in each one. The treatment was given once in a day, six of which made a course. The rats in the normal control group and aging model group were not received any treatment. After the treatment for three weeks, the rats were put to death and their spleen index, thymus index and the T lymphocytes subgroups (CD8(+) T/T cell and CD8(+) CD28(-) T/CD8(+) T cell) were tested. RESULTS: The spleen index (1.74 +/- 0.059) and thymus index (0.64 +/- 0.039) in the aging model group was obviously lower than those in the normal control group (1.93 +/- 0.061), (0.81 +/- 0.053) respectively (both P < 0.05); the CD8(+) CD28(-) T/CD8(+) T cell percentages (26.28 +/- 4.69)% and CD8(+) T/T cell percentages (43.33 +/- 2.84)% in the aging model group were both significantly higher than those (15.08 +/- 5.58)% (P < 0.01), (34.70 +/- 4.24)% (P < 0.01) in the normal control group. Compared with the aging model group, the spleen index (1.91 +/- 0.081) and thymus index (0.79 +/- 0.080) in the Shuanggu Yitong group were significantly higher (both P < 0.05), but obviously decreased with the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73) (P < 0.01); the percentage of CD8(+) CD28(-) T/CD8(+) T cell (18.07 +/- 1.73)% in the acupuncture control group was also lower than the aging model group (P < 0.05), but more obvious reduce for the Shuanggu Yitong group (P < 0.05). CONCLUSION: The treatment of Shuanggu Yitong prescription could regulate the proportions of the T lymphocyte subset, and slow down the immunosenescence of subacute aging model rats induced by D-galactose. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/22650133/[Effects_of_electroacupuncture_of_"Shuanggu_Yitong"_prescription_on_the_T_lymphocyte_subset_proportions_in_aging_rats]_ L2 - https://medlineplus.gov/olderadulthealth.html DB - PRIME DP - Unbound Medicine ER -