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In vitro and in vivo isolation and characterization of Duvenhage virus.
PLoS Pathog. 2012; 8(5):e1002682.PP

Abstract

A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV.

Authors+Show Affiliations

Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands. p.koraka@erasmusmc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22654660

Citation

Koraka, Penelope, et al. "In Vitro and in Vivo Isolation and Characterization of Duvenhage Virus." PLoS Pathogens, vol. 8, no. 5, 2012, pp. e1002682.
Koraka P, Martina BE, Roose JM, et al. In vitro and in vivo isolation and characterization of Duvenhage virus. PLoS Pathog. 2012;8(5):e1002682.
Koraka, P., Martina, B. E., Roose, J. M., van Thiel, P. P., van Amerongen, G., Kuiken, T., & Osterhaus, A. D. (2012). In vitro and in vivo isolation and characterization of Duvenhage virus. PLoS Pathogens, 8(5), e1002682. https://doi.org/10.1371/journal.ppat.1002682
Koraka P, et al. In Vitro and in Vivo Isolation and Characterization of Duvenhage Virus. PLoS Pathog. 2012;8(5):e1002682. PubMed PMID: 22654660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro and in vivo isolation and characterization of Duvenhage virus. AU - Koraka,Penelope, AU - Martina,Byron E E, AU - Roose,Jouke M, AU - van Thiel,Pieter-Paul A M, AU - van Amerongen,Geert, AU - Kuiken,Thijs, AU - Osterhaus,Albert D M E, Y1 - 2012/05/24/ PY - 2011/11/04/received PY - 2012/03/22/accepted PY - 2012/6/2/entrez PY - 2012/6/2/pubmed PY - 2012/11/9/medline SP - e1002682 EP - e1002682 JF - PLoS pathogens JO - PLoS Pathog VL - 8 IS - 5 N2 - A fatal human case of Duvenhage virus (DUVV) infection in a Dutch traveller who had returned from Kenya was reported in 2007. She exhibited classical symptoms of rabies encephalitis with distinct pathological findings. In the present study we describe the isolation and characterization of DUVV in vitro and its passage in BALB/c mice. The virus proved to be neuroinvasive in both juvenile and adult mice, resulting in about 50% lethality upon peripheral infection. Clinical signs in infected mice were those of classical rabies. However, the distribution of viral antigen expression in the brain differed from that of classical rabies virus infection and neither inclusion bodies nor neuronal necrosis were observed. This is the first study to describe the in vitro and in vivo isolation and characterization of DUVV. SN - 1553-7374 UR - https://www.unboundmedicine.com/medline/citation/22654660/In_vitro_and_in_vivo_isolation_and_characterization_of_Duvenhage_virus_ L2 - https://dx.plos.org/10.1371/journal.ppat.1002682 DB - PRIME DP - Unbound Medicine ER -