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TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts.
Cell Calcium. 2012 Aug; 52(2):124-36.CC

Abstract

Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological and pharmacological properties of TRPV1 and its functional coupling with cannabinoid (CB) receptors and Na(+)-Ca(2+) exchangers (NCXs) in odontoblasts. Anandamide (AEA), capsaicin (CAP), resiniferatoxin (RF) or low-pH evoked Ca(2+) influx. This influx was inhibited by capsazepine (CPZ). Delay in time-to-activation of TRPV1 channels was observed between application of AEA or CAP and increase in [Ca(2+)](i). In the absence of extracellular Ca(2+), however, an immediate increase in [Ca(2+)](i) was observed on administration of extracellular Ca(2+), followed by activation of TRPV1 channels. Intracellular application of CAP elicited inward current via opening of TRPV1 channels faster than extracellular application. With extracellular RF application, no time delay was observed in either increase in [Ca(2+)](i) or inward current, indicating that agonist binding sites are located on both extra- and intracellular domains. KB-R7943, an NCX inhibitor, yielded an increase in the decay time constant during TRPV1-mediated Ca(2+) entry. Increase in [Ca(2+)](i) by CB receptor agonist, 2-arachidonylglycerol, was inhibited by CB1 receptor antagonist or CPZ, as well as by adenylyl cyclase inhibitor. These results showed that TRPV1-mediated Ca(2+) entry functionally couples with CB1 receptor activation via cAMP signaling. Increased [Ca(2+)](i) by TRPV1 activation was extruded by NCXs. Taken together, this suggests that cAMP-mediated CB1-TRPV1 crosstalk and TRPV1-NCX coupling play an important role in driving cellular functions following transduction of external stimuli to odontoblasts.

Authors+Show Affiliations

Oral Health Science Center hrc8, Tokyo Dental College, Chiba, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22656960

Citation

Tsumura, Maki, et al. "TRPV1-mediated Calcium Signal Couples With Cannabinoid Receptors and Sodium-calcium Exchangers in Rat Odontoblasts." Cell Calcium, vol. 52, no. 2, 2012, pp. 124-36.
Tsumura M, Sobhan U, Muramatsu T, et al. TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. Cell Calcium. 2012;52(2):124-36.
Tsumura, M., Sobhan, U., Muramatsu, T., Sato, M., Ichikawa, H., Sahara, Y., Tazaki, M., & Shibukawa, Y. (2012). TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. Cell Calcium, 52(2), 124-36. https://doi.org/10.1016/j.ceca.2012.05.002
Tsumura M, et al. TRPV1-mediated Calcium Signal Couples With Cannabinoid Receptors and Sodium-calcium Exchangers in Rat Odontoblasts. Cell Calcium. 2012;52(2):124-36. PubMed PMID: 22656960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts. AU - Tsumura,Maki, AU - Sobhan,Ubaidus, AU - Muramatsu,Takashi, AU - Sato,Masaki, AU - Ichikawa,Hideki, AU - Sahara,Yoshinori, AU - Tazaki,Masakazu, AU - Shibukawa,Yoshiyuki, Y1 - 2012/05/30/ PY - 2011/12/30/received PY - 2012/04/24/revised PY - 2012/05/02/accepted PY - 2012/6/5/entrez PY - 2012/6/5/pubmed PY - 2012/10/31/medline SP - 124 EP - 36 JF - Cell calcium JO - Cell Calcium VL - 52 IS - 2 N2 - Odontoblasts are involved in the transduction of stimuli applied to exposed dentin. Although expression of thermo/mechano/osmo-sensitive transient receptor potential (TRP) channels has been demonstrated, the properties of TRP vanilloid 1 (TRPV1)-mediated signaling remain to be clarified. We investigated physiological and pharmacological properties of TRPV1 and its functional coupling with cannabinoid (CB) receptors and Na(+)-Ca(2+) exchangers (NCXs) in odontoblasts. Anandamide (AEA), capsaicin (CAP), resiniferatoxin (RF) or low-pH evoked Ca(2+) influx. This influx was inhibited by capsazepine (CPZ). Delay in time-to-activation of TRPV1 channels was observed between application of AEA or CAP and increase in [Ca(2+)](i). In the absence of extracellular Ca(2+), however, an immediate increase in [Ca(2+)](i) was observed on administration of extracellular Ca(2+), followed by activation of TRPV1 channels. Intracellular application of CAP elicited inward current via opening of TRPV1 channels faster than extracellular application. With extracellular RF application, no time delay was observed in either increase in [Ca(2+)](i) or inward current, indicating that agonist binding sites are located on both extra- and intracellular domains. KB-R7943, an NCX inhibitor, yielded an increase in the decay time constant during TRPV1-mediated Ca(2+) entry. Increase in [Ca(2+)](i) by CB receptor agonist, 2-arachidonylglycerol, was inhibited by CB1 receptor antagonist or CPZ, as well as by adenylyl cyclase inhibitor. These results showed that TRPV1-mediated Ca(2+) entry functionally couples with CB1 receptor activation via cAMP signaling. Increased [Ca(2+)](i) by TRPV1 activation was extruded by NCXs. Taken together, this suggests that cAMP-mediated CB1-TRPV1 crosstalk and TRPV1-NCX coupling play an important role in driving cellular functions following transduction of external stimuli to odontoblasts. SN - 1532-1991 UR - https://www.unboundmedicine.com/medline/citation/22656960/TRPV1_mediated_calcium_signal_couples_with_cannabinoid_receptors_and_sodium_calcium_exchangers_in_rat_odontoblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(12)00089-9 DB - PRIME DP - Unbound Medicine ER -