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Protective effect of Fufang-Liu-Yue-Qing, a traditional Chinese herbal formula, on CCl4 induced liver fibrosis in rats.
J Ethnopharmacol. 2012 Jul 13; 142(2):548-56.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Chinese prescription Fufang-Liu-Yue-Qing (FLYQ) has long been employed clinically to treat chronic hepatitis B, and we have reported its beneficial effects on liver fibrosis in vitro. The present study was investigated to verify protective effects of FLYQ on liver fibrosis in a rat model and to investigate the underlying mechanisms which have not been explored yet.

MATERIALS AND METHODS

Liver fibrosis was established by intragastric administration of 2 ml/kg CCl(4) twice a week for 12 weeks. During the experiment, the model group received CCl(4) only, and the normal control group received an equal volume of saline. Treatment groups received not only CCl(4) for 12 weeks, but also the corresponding drugs, colchicine (1.00 mg/kg/day) or FLYQ (300, 150, 75 mg/kg/day) from 5 to 12 weeks.

RESULTS

Analysis experiments showed that FLYQ could significantly alleviate liver injury, as indicated by decreasing levels of ALT, AST, ALP, GGT, IL-6 and TNF-α. Moreover, FLYQ could effectively inhibit collagen deposition and reduce the pathological tissue damage. Research on mechanism showed that FLYQ was able to markedly reduce lipid peroxidation, recruit the anti-oxidative defense system, promote ECM degradation by modulating the levels of TIMP-1 and MMP-2, and induce HSC apoptosis by down-regulating bcl-2 mRNA, as well as inhibit the expressions of α-SMA and TGF-β(1) proteins.

CONCLUSIONS

Our results show that FLYQ is effective in attenuating hepatic injury and fibrosis in the CCl(4)-induced rat model, which should be developed as a new drug for treatment of liver fibrosis and even cirrhosis.

Authors+Show Affiliations

Guangxi Medical University, Nanning 530021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22658988

Citation

Lin, Xing, et al. "Protective Effect of Fufang-Liu-Yue-Qing, a Traditional Chinese Herbal Formula, On CCl4 Induced Liver Fibrosis in Rats." Journal of Ethnopharmacology, vol. 142, no. 2, 2012, pp. 548-56.
Lin X, Zhang S, Huang Q, et al. Protective effect of Fufang-Liu-Yue-Qing, a traditional Chinese herbal formula, on CCl4 induced liver fibrosis in rats. J Ethnopharmacol. 2012;142(2):548-56.
Lin, X., Zhang, S., Huang, Q., Wei, L., Zheng, L., Chen, Z., Jiao, Y., Huang, J., Fu, S., & Huang, R. (2012). Protective effect of Fufang-Liu-Yue-Qing, a traditional Chinese herbal formula, on CCl4 induced liver fibrosis in rats. Journal of Ethnopharmacology, 142(2), 548-56. https://doi.org/10.1016/j.jep.2012.05.040
Lin X, et al. Protective Effect of Fufang-Liu-Yue-Qing, a Traditional Chinese Herbal Formula, On CCl4 Induced Liver Fibrosis in Rats. J Ethnopharmacol. 2012 Jul 13;142(2):548-56. PubMed PMID: 22658988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of Fufang-Liu-Yue-Qing, a traditional Chinese herbal formula, on CCl4 induced liver fibrosis in rats. AU - Lin,Xing, AU - Zhang,Shijun, AU - Huang,Quanfang, AU - Wei,Ling, AU - Zheng,Li, AU - Chen,Zhaoni, AU - Jiao,Yang, AU - Huang,Jianchun, AU - Fu,Shujie, AU - Huang,Renbin, Y1 - 2012/05/31/ PY - 2012/05/21/received PY - 2012/05/23/accepted PY - 2012/6/5/entrez PY - 2012/6/5/pubmed PY - 2012/10/24/medline SP - 548 EP - 56 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Chinese prescription Fufang-Liu-Yue-Qing (FLYQ) has long been employed clinically to treat chronic hepatitis B, and we have reported its beneficial effects on liver fibrosis in vitro. The present study was investigated to verify protective effects of FLYQ on liver fibrosis in a rat model and to investigate the underlying mechanisms which have not been explored yet. MATERIALS AND METHODS: Liver fibrosis was established by intragastric administration of 2 ml/kg CCl(4) twice a week for 12 weeks. During the experiment, the model group received CCl(4) only, and the normal control group received an equal volume of saline. Treatment groups received not only CCl(4) for 12 weeks, but also the corresponding drugs, colchicine (1.00 mg/kg/day) or FLYQ (300, 150, 75 mg/kg/day) from 5 to 12 weeks. RESULTS: Analysis experiments showed that FLYQ could significantly alleviate liver injury, as indicated by decreasing levels of ALT, AST, ALP, GGT, IL-6 and TNF-α. Moreover, FLYQ could effectively inhibit collagen deposition and reduce the pathological tissue damage. Research on mechanism showed that FLYQ was able to markedly reduce lipid peroxidation, recruit the anti-oxidative defense system, promote ECM degradation by modulating the levels of TIMP-1 and MMP-2, and induce HSC apoptosis by down-regulating bcl-2 mRNA, as well as inhibit the expressions of α-SMA and TGF-β(1) proteins. CONCLUSIONS: Our results show that FLYQ is effective in attenuating hepatic injury and fibrosis in the CCl(4)-induced rat model, which should be developed as a new drug for treatment of liver fibrosis and even cirrhosis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22658988/Protective_effect_of_Fufang_Liu_Yue_Qing_a_traditional_Chinese_herbal_formula_on_CCl4_induced_liver_fibrosis_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00350-9 DB - PRIME DP - Unbound Medicine ER -