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VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK.
Exp Cell Res. 2012 Aug 15; 318(14):1633-40.EC

Abstract

Vascular endothelial growth factor (VEGF) is one of the strongest regulators of physiological and pathological angiogenesis. VEGF receptor 2 (VEGFR-2), the primary receptor for VEGF, is thought to mediate major functional effects of VEGF. Previously, we have localized both VEGF and VEGFR-2 in human hair follicles. In this study, we further defined the expression and roles of VEGFR-2 on human hair follicle dermal papilla (DP) cells. The expression of VEGFR-2 on DP cells was examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis separately, and localization of VEGFR-2 was defined by immunofluorescence. The effect of VEGF on DP cells was analyzed by MTT assays and specific inhibitors. Finally, the role of VEGF involved in the signaling pathways was investigated by Western blot. RT-PCR and Western blot analysis demonstrated the expression of VEGFR-2 on DP cells. Immunostaining for VEGFR-2 showed strong signal on cultured human DP cells in vitro. Exogenous VEGF(165) stimulated proliferation of DP cells in a dose-dependent manner. Furthermore, this stimulation was blocked by a VEGFR-2 neutralizing antibody (MAB3571) and an ERK inhibitor (PD98059). VEGF(165)-induced phosphorylation of ERK1/2 was abolished by MAB3571 and PD98059, while the phosphorylation of p38, JNK and AKT were not changed by VEGF(165). Taken together, VEGFR-2 is expressed on primary human hair follicle DP cells and VEGF induces proliferation of DP cells through VEGFR-2/ERK pathway, but not p38, JNK or AKT signaling.

Authors+Show Affiliations

Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22659165

Citation

Li, Wei, et al. "VEGF Induces Proliferation of Human Hair Follicle Dermal Papilla Cells Through VEGFR-2-mediated Activation of ERK." Experimental Cell Research, vol. 318, no. 14, 2012, pp. 1633-40.
Li W, Man XY, Li CM, et al. VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK. Exp Cell Res. 2012;318(14):1633-40.
Li, W., Man, X. Y., Li, C. M., Chen, J. Q., Zhou, J., Cai, S. Q., Lu, Z. F., & Zheng, M. (2012). VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK. Experimental Cell Research, 318(14), 1633-40. https://doi.org/10.1016/j.yexcr.2012.05.003
Li W, et al. VEGF Induces Proliferation of Human Hair Follicle Dermal Papilla Cells Through VEGFR-2-mediated Activation of ERK. Exp Cell Res. 2012 Aug 15;318(14):1633-40. PubMed PMID: 22659165.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK. AU - Li,Wei, AU - Man,Xiao-Yong, AU - Li,Chun-Ming, AU - Chen,Jia-Qi, AU - Zhou,Jiong, AU - Cai,Sui-Qing, AU - Lu,Zhong-Fa, AU - Zheng,Min, Y1 - 2012/05/29/ PY - 2011/12/15/received PY - 2012/04/29/revised PY - 2012/05/01/accepted PY - 2012/6/5/entrez PY - 2012/6/5/pubmed PY - 2012/9/15/medline SP - 1633 EP - 40 JF - Experimental cell research JO - Exp Cell Res VL - 318 IS - 14 N2 - Vascular endothelial growth factor (VEGF) is one of the strongest regulators of physiological and pathological angiogenesis. VEGF receptor 2 (VEGFR-2), the primary receptor for VEGF, is thought to mediate major functional effects of VEGF. Previously, we have localized both VEGF and VEGFR-2 in human hair follicles. In this study, we further defined the expression and roles of VEGFR-2 on human hair follicle dermal papilla (DP) cells. The expression of VEGFR-2 on DP cells was examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis separately, and localization of VEGFR-2 was defined by immunofluorescence. The effect of VEGF on DP cells was analyzed by MTT assays and specific inhibitors. Finally, the role of VEGF involved in the signaling pathways was investigated by Western blot. RT-PCR and Western blot analysis demonstrated the expression of VEGFR-2 on DP cells. Immunostaining for VEGFR-2 showed strong signal on cultured human DP cells in vitro. Exogenous VEGF(165) stimulated proliferation of DP cells in a dose-dependent manner. Furthermore, this stimulation was blocked by a VEGFR-2 neutralizing antibody (MAB3571) and an ERK inhibitor (PD98059). VEGF(165)-induced phosphorylation of ERK1/2 was abolished by MAB3571 and PD98059, while the phosphorylation of p38, JNK and AKT were not changed by VEGF(165). Taken together, VEGFR-2 is expressed on primary human hair follicle DP cells and VEGF induces proliferation of DP cells through VEGFR-2/ERK pathway, but not p38, JNK or AKT signaling. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/22659165/VEGF_induces_proliferation_of_human_hair_follicle_dermal_papilla_cells_through_VEGFR_2_mediated_activation_of_ERK_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(12)00241-8 DB - PRIME DP - Unbound Medicine ER -