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Genomic organization and sequence dynamics of the AvrPiz-t locus in Magnaporthe oryzae.
J Zhejiang Univ Sci B. 2012 Jun; 13(6):452-64.JZ

Abstract

Plants utilize multiple layers of defense mechanisms to fight against the invasion of diverse pathogens. The R gene mediates resistance, in most cases, dependent on the co-existence of its cognate pathogen-derived avirulence (Avr) gene. The rice blast R gene Piz-t corresponds in gene-for-gene fashion to the Magnaporthe oryzae Avr gene AvrPiz-t. In this study, we determined and compared the genomic sequences surrounding the AvrPiz-t gene in both avirulent and virulent isolates, designating as AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively. The sequence of the AvrPiz-t-ZB15 region is 120966 bp whereas avrPiz-t-70-15 is 146292 bp in length. The extreme sequence similarity and good synteny in gene order and content along with the absence of two predicted genes in the avrPiz-t-70-15 region were observed in the predicted protein-coding regions in the AvrPiz-t locus. Nevertheless, frequent presence/absence and highly dynamic organization of transposable elements (TEs) were identified, representing the major variation of the AvrPiz-t locus between different isolates. Moreover, TEs constitute 27.3% and 43.2% of the genomic contents of the AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively, indicating that TEs contribute largely to the organization and evolution of AvrPiz-t locus. The findings of this study suggest that M. oryzae could benefit in an evolutionary sense from the presence of active TEs in genes conferring avirulence and provide an ability to rapidly change and thus to overcome host R genes.

Authors+Show Affiliations

State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22661208

Citation

Li, Ping, et al. "Genomic Organization and Sequence Dynamics of the AvrPiz-t Locus in Magnaporthe Oryzae." Journal of Zhejiang University. Science. B, vol. 13, no. 6, 2012, pp. 452-64.
Li P, Bai B, Zhang HY, et al. Genomic organization and sequence dynamics of the AvrPiz-t locus in Magnaporthe oryzae. J Zhejiang Univ Sci B. 2012;13(6):452-64.
Li, P., Bai, B., Zhang, H. Y., Zhou, H., & Zhou, B. (2012). Genomic organization and sequence dynamics of the AvrPiz-t locus in Magnaporthe oryzae. Journal of Zhejiang University. Science. B, 13(6), 452-64. https://doi.org/10.1631/jzus.B1100338
Li P, et al. Genomic Organization and Sequence Dynamics of the AvrPiz-t Locus in Magnaporthe Oryzae. J Zhejiang Univ Sci B. 2012;13(6):452-64. PubMed PMID: 22661208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic organization and sequence dynamics of the AvrPiz-t locus in Magnaporthe oryzae. AU - Li,Ping, AU - Bai,Bin, AU - Zhang,Hong-yan, AU - Zhou,Heng, AU - Zhou,Bo, PY - 2012/6/5/entrez PY - 2012/6/5/pubmed PY - 2012/10/10/medline SP - 452 EP - 64 JF - Journal of Zhejiang University. Science. B JO - J Zhejiang Univ Sci B VL - 13 IS - 6 N2 - Plants utilize multiple layers of defense mechanisms to fight against the invasion of diverse pathogens. The R gene mediates resistance, in most cases, dependent on the co-existence of its cognate pathogen-derived avirulence (Avr) gene. The rice blast R gene Piz-t corresponds in gene-for-gene fashion to the Magnaporthe oryzae Avr gene AvrPiz-t. In this study, we determined and compared the genomic sequences surrounding the AvrPiz-t gene in both avirulent and virulent isolates, designating as AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively. The sequence of the AvrPiz-t-ZB15 region is 120966 bp whereas avrPiz-t-70-15 is 146292 bp in length. The extreme sequence similarity and good synteny in gene order and content along with the absence of two predicted genes in the avrPiz-t-70-15 region were observed in the predicted protein-coding regions in the AvrPiz-t locus. Nevertheless, frequent presence/absence and highly dynamic organization of transposable elements (TEs) were identified, representing the major variation of the AvrPiz-t locus between different isolates. Moreover, TEs constitute 27.3% and 43.2% of the genomic contents of the AvrPiz-t-ZB15 and avrPiz-t-70-15 regions, respectively, indicating that TEs contribute largely to the organization and evolution of AvrPiz-t locus. The findings of this study suggest that M. oryzae could benefit in an evolutionary sense from the presence of active TEs in genes conferring avirulence and provide an ability to rapidly change and thus to overcome host R genes. SN - 1862-1783 UR - https://www.unboundmedicine.com/medline/citation/22661208/Genomic_organization_and_sequence_dynamics_of_the_AvrPiz_t_locus_in_Magnaporthe_oryzae_ L2 - http://www.jzus.zju.edu.cn/article.php?doi=10.1631/jzus.B1100338 DB - PRIME DP - Unbound Medicine ER -