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Induction of DNA fragmentation, chromosome aberrations and micronuclei by cisplatin in rat bone-marrow cells: protective effect of recombinant human erythropoietin.
Mutat Res. 2012 Sep 18; 747(2):202-6.MR

Abstract

Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses several side effects may occur. Recombinant human erythropoietin (rhEPO), a glycoprotein regulating haematopoiesis, has recently been shown to exert an important cyto-protective effects in many tissues. The purpose of this study was to explore whether rhEPO protects against Cisp-induced genotoxicity in rat bone-marrow cells. Adult male Wistar rats were divided into six groups of 18 animals each: control group, rhEPO-alone group, Cisp-alone group and three rhEPO+Cisp-groups (pre-, co- and post-treatment condition, respectively). Our results show that Cisp induced a noticeable genotoxic effect in rat bone-marrow cells. In all types of treatment, rhEPO significantly decreased the frequency of micronuclei, the percentage of chromosome aberrations and the level of DNA damage. The protective effect of rhEPO was more efficient when it was administrated 24h before exposure to Cisp.

Authors+Show Affiliations

Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, Monastir, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22664391

Citation

Rjiba-Touati, Karima, et al. "Induction of DNA Fragmentation, Chromosome Aberrations and Micronuclei By Cisplatin in Rat Bone-marrow Cells: Protective Effect of Recombinant Human Erythropoietin." Mutation Research, vol. 747, no. 2, 2012, pp. 202-6.
Rjiba-Touati K, Ayed-Boussema I, Skhiri H, et al. Induction of DNA fragmentation, chromosome aberrations and micronuclei by cisplatin in rat bone-marrow cells: protective effect of recombinant human erythropoietin. Mutat Res. 2012;747(2):202-6.
Rjiba-Touati, K., Ayed-Boussema, I., Skhiri, H., Belarbia, A., Zellema, D., Achour, A., & Bacha, H. (2012). Induction of DNA fragmentation, chromosome aberrations and micronuclei by cisplatin in rat bone-marrow cells: protective effect of recombinant human erythropoietin. Mutation Research, 747(2), 202-6. https://doi.org/10.1016/j.mrgentox.2012.05.011
Rjiba-Touati K, et al. Induction of DNA Fragmentation, Chromosome Aberrations and Micronuclei By Cisplatin in Rat Bone-marrow Cells: Protective Effect of Recombinant Human Erythropoietin. Mutat Res. 2012 Sep 18;747(2):202-6. PubMed PMID: 22664391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of DNA fragmentation, chromosome aberrations and micronuclei by cisplatin in rat bone-marrow cells: protective effect of recombinant human erythropoietin. AU - Rjiba-Touati,Karima, AU - Ayed-Boussema,Imen, AU - Skhiri,Habib, AU - Belarbia,Anis, AU - Zellema,Dorsaf, AU - Achour,Abdelatif, AU - Bacha,Hassen, Y1 - 2012/06/01/ PY - 2011/10/22/received PY - 2012/03/07/revised PY - 2012/05/10/accepted PY - 2012/6/6/entrez PY - 2012/6/6/pubmed PY - 2012/9/19/medline SP - 202 EP - 6 JF - Mutation research JO - Mutat Res VL - 747 IS - 2 N2 - Cisplatin (Cisp) is one of the most effective chemotherapeutic agents. However, at higher doses several side effects may occur. Recombinant human erythropoietin (rhEPO), a glycoprotein regulating haematopoiesis, has recently been shown to exert an important cyto-protective effects in many tissues. The purpose of this study was to explore whether rhEPO protects against Cisp-induced genotoxicity in rat bone-marrow cells. Adult male Wistar rats were divided into six groups of 18 animals each: control group, rhEPO-alone group, Cisp-alone group and three rhEPO+Cisp-groups (pre-, co- and post-treatment condition, respectively). Our results show that Cisp induced a noticeable genotoxic effect in rat bone-marrow cells. In all types of treatment, rhEPO significantly decreased the frequency of micronuclei, the percentage of chromosome aberrations and the level of DNA damage. The protective effect of rhEPO was more efficient when it was administrated 24h before exposure to Cisp. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/22664391/Induction_of_DNA_fragmentation_chromosome_aberrations_and_micronuclei_by_cisplatin_in_rat_bone_marrow_cells:_protective_effect_of_recombinant_human_erythropoietin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(12)00198-2 DB - PRIME DP - Unbound Medicine ER -