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Salidroside protects PC12 cells from MPP⁺-induced apoptosis via activation of the PI3K/Akt pathway.
Food Chem Toxicol. 2012 Aug; 50(8):2591-7.FC

Abstract

Oxidative stress plays an important role in the pathogenesis of Parkinson's disease (PD). Salidroside (SAL), a phenylpropanoid glycoside isolated from Rhodiola rosea L., can exert potent antioxidant properties. In this study, we investigated the protective effects, and the possible mechanism of action, of SAL against 1-methyl-4-phenylpyridinium (MPP(+))-induced cell damage in rat adrenal pheochromocytoma PC12 cells. Pretreatment of PC12 cells with SAL significantly reduced the ability of MPP(+) to induce apoptosis in a dose and time-dependent manner. SAL significantly and dose-dependently inhibited MPP(+)-induced chromatin condensation and MPP(+)-induced release of lactate dehydrogenase by PC12 cells. SAL enhanced Akt phosphorylation in PC12 cells, and the protective effects of SAL against MPP(+)-induced apoptosis were abolished by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K) phosphorylation. These findings suggest that SAL prevents MPP(+)-induced apoptosis in PC12 cells, at least in part through activation of the PI3K/Akt pathway.

Authors+Show Affiliations

Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22664423

Citation

Zhang, Lingling, et al. "Salidroside Protects PC12 Cells From MPP⁺-induced Apoptosis Via Activation of the PI3K/Akt Pathway." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 50, no. 8, 2012, pp. 2591-7.
Zhang L, Ding W, Sun H, et al. Salidroside protects PC12 cells from MPP⁺-induced apoptosis via activation of the PI3K/Akt pathway. Food Chem Toxicol. 2012;50(8):2591-7.
Zhang, L., Ding, W., Sun, H., Zhou, Q., Huang, J., Li, X., Xie, Y., & Chen, J. (2012). Salidroside protects PC12 cells from MPP⁺-induced apoptosis via activation of the PI3K/Akt pathway. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 50(8), 2591-7. https://doi.org/10.1016/j.fct.2012.05.045
Zhang L, et al. Salidroside Protects PC12 Cells From MPP⁺-induced Apoptosis Via Activation of the PI3K/Akt Pathway. Food Chem Toxicol. 2012;50(8):2591-7. PubMed PMID: 22664423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Salidroside protects PC12 cells from MPP⁺-induced apoptosis via activation of the PI3K/Akt pathway. AU - Zhang,Lingling, AU - Ding,Wenjun, AU - Sun,Huixing, AU - Zhou,Qiong, AU - Huang,Jingqun, AU - Li,Xuefen, AU - Xie,Yonghong, AU - Chen,Jianzong, Y1 - 2012/06/01/ PY - 2012/01/06/received PY - 2012/05/22/revised PY - 2012/05/25/accepted PY - 2012/6/6/entrez PY - 2012/6/6/pubmed PY - 2012/12/10/medline SP - 2591 EP - 7 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 50 IS - 8 N2 - Oxidative stress plays an important role in the pathogenesis of Parkinson's disease (PD). Salidroside (SAL), a phenylpropanoid glycoside isolated from Rhodiola rosea L., can exert potent antioxidant properties. In this study, we investigated the protective effects, and the possible mechanism of action, of SAL against 1-methyl-4-phenylpyridinium (MPP(+))-induced cell damage in rat adrenal pheochromocytoma PC12 cells. Pretreatment of PC12 cells with SAL significantly reduced the ability of MPP(+) to induce apoptosis in a dose and time-dependent manner. SAL significantly and dose-dependently inhibited MPP(+)-induced chromatin condensation and MPP(+)-induced release of lactate dehydrogenase by PC12 cells. SAL enhanced Akt phosphorylation in PC12 cells, and the protective effects of SAL against MPP(+)-induced apoptosis were abolished by LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K) phosphorylation. These findings suggest that SAL prevents MPP(+)-induced apoptosis in PC12 cells, at least in part through activation of the PI3K/Akt pathway. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/22664423/Salidroside_protects_PC12_cells_from_MPP⁺_induced_apoptosis_via_activation_of_the_PI3K/Akt_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(12)00388-2 DB - PRIME DP - Unbound Medicine ER -