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[Simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field using high performance liquid chromatography].
Se Pu. 2012 Jan; 30(1):71-5.SP

Abstract

An analytical method of high performance liquid chromatography (HPLC) was established for the simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field (paddy water, soil and rice plant). The residues in the paddy water was extracted with methylene chloride, and the soil with alkaline mixed solution of acetonitrile-methylene chloride (1:1, v/v). The rice plant was extracted with alkaline methylene chloride which was cleaned up by a Florisil column. The separation was performed on a stainless steel C18 column (150 mm x 4.6 mm, 5 microm) at 30 degrees C by HPLC with an ultraviolet detector (UVD) at 238 nm, and water-methanol (30:70, v/v) as the mobile phase at a flow rate of 0.5 mL/min. The quantification was performed by external standard. The calibration curves were linear between the peak area and the concentration in the range of 0.05-5.00 mg/L for bensulfuron-methyl and mefenacet, and the correlation coefficients were more than 0.999 9. The average recoveries of the two herbicides spiked in the paddy water, soil and rice plant at the three concentration levels of 0.05, 0.10 and 1.00 mg/kg ranged from 85.39% to 113.33% with the relative standard deviations of 0.91%-10.24%. The method is characterized by simplicity, sensitivity and accuracy.

Authors+Show Affiliations

Yang L
College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China.
Gong D
No affiliation info available
Tang J
No affiliation info available
Luo J
No affiliation info available
Ding C
No affiliation info available

MeSH

AcetanilidesBenzothiazolesChromatography, High Pressure LiquidFood ContaminationHerbicidesOryzaPesticide ResiduesSoilSoil PollutantsSulfonylurea Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22667095

Citation

Yang, Lihua, et al. "[Simultaneous Determination of Bensulfuron-methyl and Mefenacet Residues in Paddy Field Using High Performance Liquid Chromatography]." Se Pu = Chinese Journal of Chromatography, vol. 30, no. 1, 2012, pp. 71-5.
Yang L, Gong D, Tang J, et al. [Simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field using high performance liquid chromatography]. Se Pu. 2012;30(1):71-5.
Yang, L., Gong, D., Tang, J., Luo, J., & Ding, C. (2012). [Simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field using high performance liquid chromatography]. Se Pu = Chinese Journal of Chromatography, 30(1), 71-5.
Yang L, et al. [Simultaneous Determination of Bensulfuron-methyl and Mefenacet Residues in Paddy Field Using High Performance Liquid Chromatography]. Se Pu. 2012;30(1):71-5. PubMed PMID: 22667095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field using high performance liquid chromatography]. AU - Yang,Lihua, AU - Gong,Daoxin, AU - Tang,Jing, AU - Luo,Junkai, AU - Ding,Chunxia, PY - 2012/6/7/entrez PY - 2012/6/7/pubmed PY - 2013/7/16/medline SP - 71 EP - 5 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 30 IS - 1 N2 - An analytical method of high performance liquid chromatography (HPLC) was established for the simultaneous determination of bensulfuron-methyl and mefenacet residues in paddy field (paddy water, soil and rice plant). The residues in the paddy water was extracted with methylene chloride, and the soil with alkaline mixed solution of acetonitrile-methylene chloride (1:1, v/v). The rice plant was extracted with alkaline methylene chloride which was cleaned up by a Florisil column. The separation was performed on a stainless steel C18 column (150 mm x 4.6 mm, 5 microm) at 30 degrees C by HPLC with an ultraviolet detector (UVD) at 238 nm, and water-methanol (30:70, v/v) as the mobile phase at a flow rate of 0.5 mL/min. The quantification was performed by external standard. The calibration curves were linear between the peak area and the concentration in the range of 0.05-5.00 mg/L for bensulfuron-methyl and mefenacet, and the correlation coefficients were more than 0.999 9. The average recoveries of the two herbicides spiked in the paddy water, soil and rice plant at the three concentration levels of 0.05, 0.10 and 1.00 mg/kg ranged from 85.39% to 113.33% with the relative standard deviations of 0.91%-10.24%. The method is characterized by simplicity, sensitivity and accuracy. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/22667095/[Simultaneous_determination_of_bensulfuron_methyl_and_mefenacet_residues_in_paddy_field_using_high_performance_liquid_chromatography]_ DB - PRIME DP - Unbound Medicine ER -