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A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses.
Biosens Bioelectron 2012 Oct-Dec; 38(1):145-50BB

Abstract

Traditional potentiometric polyion-sensitive electrodes can only work effectively in samples with vigorous convection fulfilled by magnetic stirrer, electrode rotator, or other moving components. The dependence on complex moving parts prohibits the fabrication of compact, cost-effective, and energy-effective test devices from the commercial point of view. In this paper, a novel potentiometric sensing protocol without using any moving parts has been proposed for polycationic protamine. In contrast to traditional protamine-sensitive electrodes conditioned by discriminated ion (Na(+)), the proposed electrode is conditioned with primary ion (protamine). Upon a medium exchange from the conditioning solution into an unstirred sample solution without protamine, protamine loaded in the membrane is stripped into the aqueous phase via ion exchange with aqueous sodium ion, thereby inducing a large potential drop. Interestingly, when the sample solution initially contains protamine, the ion-exchange process has been found to be sensitively inhibited by the sample protamine, and thus the potential drop is suppressed, which forms the basis of the moving-part-free potentiometric polyion sensing strategy. Utilizing the digestion ability of protease to protamine, the electrode was employed to determine the activity of trypsin with a detection limit at least one order of magnitude lower than traditional potentiometric methods. The trypsin inhibitor in both buffer and plasma samples was also sensitively detected with the moving-part-free protamine-sensitive electrode. Finally, the ability of the proposed electrode to detect polyanionic heparin was demonstrated.

Authors+Show Affiliations

Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22672765

Citation

Wang, Xuewei, et al. "A Moving-part-free Protamine-sensitive Polymeric Membrane Electrode for Sensitive Biomedical Analyses." Biosensors & Bioelectronics, vol. 38, no. 1, 2012, pp. 145-50.
Wang X, Wang Q, Qin W. A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses. Biosens Bioelectron. 2012;38(1):145-50.
Wang, X., Wang, Q., & Qin, W. (2012). A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses. Biosensors & Bioelectronics, 38(1), pp. 145-50. doi:10.1016/j.bios.2012.05.020.
Wang X, Wang Q, Qin W. A Moving-part-free Protamine-sensitive Polymeric Membrane Electrode for Sensitive Biomedical Analyses. Biosens Bioelectron. 2012;38(1):145-50. PubMed PMID: 22672765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A moving-part-free protamine-sensitive polymeric membrane electrode for sensitive biomedical analyses. AU - Wang,Xuewei, AU - Wang,Qing, AU - Qin,Wei, Y1 - 2012/05/24/ PY - 2012/03/26/received PY - 2012/05/06/revised PY - 2012/05/15/accepted PY - 2012/6/8/entrez PY - 2012/6/8/pubmed PY - 2012/12/12/medline SP - 145 EP - 50 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 38 IS - 1 N2 - Traditional potentiometric polyion-sensitive electrodes can only work effectively in samples with vigorous convection fulfilled by magnetic stirrer, electrode rotator, or other moving components. The dependence on complex moving parts prohibits the fabrication of compact, cost-effective, and energy-effective test devices from the commercial point of view. In this paper, a novel potentiometric sensing protocol without using any moving parts has been proposed for polycationic protamine. In contrast to traditional protamine-sensitive electrodes conditioned by discriminated ion (Na(+)), the proposed electrode is conditioned with primary ion (protamine). Upon a medium exchange from the conditioning solution into an unstirred sample solution without protamine, protamine loaded in the membrane is stripped into the aqueous phase via ion exchange with aqueous sodium ion, thereby inducing a large potential drop. Interestingly, when the sample solution initially contains protamine, the ion-exchange process has been found to be sensitively inhibited by the sample protamine, and thus the potential drop is suppressed, which forms the basis of the moving-part-free potentiometric polyion sensing strategy. Utilizing the digestion ability of protease to protamine, the electrode was employed to determine the activity of trypsin with a detection limit at least one order of magnitude lower than traditional potentiometric methods. The trypsin inhibitor in both buffer and plasma samples was also sensitively detected with the moving-part-free protamine-sensitive electrode. Finally, the ability of the proposed electrode to detect polyanionic heparin was demonstrated. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22672765/A_moving_part_free_protamine_sensitive_polymeric_membrane_electrode_for_sensitive_biomedical_analyses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00313-2 DB - PRIME DP - Unbound Medicine ER -