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Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei.
PLoS One. 2012; 7(6):e37762.Plos

Abstract

Burkholderia pseudomallei, as a saprophytic bacterium that can cause a severe sepsis disease named melioidosis, has preserved several extra genes in its genome for survival. The sequenced genome of the organism showed high diversity contributed mainly from genomic islands (GIs). Comparative genome hybridization (CGH) of 3 clinical and 2 environmental isolates, using whole genome microarrays based on B. pseudomallei K96243 genes, revealed a difference in the presence of genomic islands between clinical and environmental isolates. The largest GI, GI8, of B. pseudomallei was observed as a 2 sub-GI named GIs8.1 and 8.2 with distinguishable %GC content and unequal presence in the genome. GIs8.1, 8.2 and 15 were found to be more common in clinical isolates. A new GI, GI16c, was detected on chromosome 2. Presences of GIs8.1, 8.2, 15 and 16c were evaluated in 70 environmental and 64 clinical isolates using PCR assays. A combination of GIs8.1 and 16c (positivity of either GI) was detected in 70% of clinical isolates and 11.4% of environmental isolates (P<0.001). Using BALB/c mice model, no significant difference of time to mortality was observed between K96243 isolate and three isolates without GIs under evaluation (P>0.05). Some virulence genes located in the absent GIs and the difference of GIs seems to contribute less to bacterial virulence. The PCR detection of 2 GIs could be used as a cost effective and rapid tool to detect potentially virulent isolates that were contaminated in soil.

Authors+Show Affiliations

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22675491

Citation

Bartpho, Thanatchaporn, et al. "Genomic Islands as a Marker to Differentiate Between Clinical and Environmental Burkholderia Pseudomallei." PloS One, vol. 7, no. 6, 2012, pp. e37762.
Bartpho T, Wongsurawat T, Wongratanacheewin S, et al. Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. PLoS One. 2012;7(6):e37762.
Bartpho, T., Wongsurawat, T., Wongratanacheewin, S., Talaat, A. M., Karoonuthaisiri, N., & Sermswan, R. W. (2012). Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. PloS One, 7(6), e37762. https://doi.org/10.1371/journal.pone.0037762
Bartpho T, et al. Genomic Islands as a Marker to Differentiate Between Clinical and Environmental Burkholderia Pseudomallei. PLoS One. 2012;7(6):e37762. PubMed PMID: 22675491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. AU - Bartpho,Thanatchaporn, AU - Wongsurawat,Thidathip, AU - Wongratanacheewin,Surasakdi, AU - Talaat,Adel M, AU - Karoonuthaisiri,Nitsara, AU - Sermswan,Rasana W, Y1 - 2012/06/01/ PY - 2011/11/17/received PY - 2012/04/24/accepted PY - 2012/6/8/entrez PY - 2012/6/8/pubmed PY - 2012/10/12/medline SP - e37762 EP - e37762 JF - PloS one JO - PLoS One VL - 7 IS - 6 N2 - Burkholderia pseudomallei, as a saprophytic bacterium that can cause a severe sepsis disease named melioidosis, has preserved several extra genes in its genome for survival. The sequenced genome of the organism showed high diversity contributed mainly from genomic islands (GIs). Comparative genome hybridization (CGH) of 3 clinical and 2 environmental isolates, using whole genome microarrays based on B. pseudomallei K96243 genes, revealed a difference in the presence of genomic islands between clinical and environmental isolates. The largest GI, GI8, of B. pseudomallei was observed as a 2 sub-GI named GIs8.1 and 8.2 with distinguishable %GC content and unequal presence in the genome. GIs8.1, 8.2 and 15 were found to be more common in clinical isolates. A new GI, GI16c, was detected on chromosome 2. Presences of GIs8.1, 8.2, 15 and 16c were evaluated in 70 environmental and 64 clinical isolates using PCR assays. A combination of GIs8.1 and 16c (positivity of either GI) was detected in 70% of clinical isolates and 11.4% of environmental isolates (P<0.001). Using BALB/c mice model, no significant difference of time to mortality was observed between K96243 isolate and three isolates without GIs under evaluation (P>0.05). Some virulence genes located in the absent GIs and the difference of GIs seems to contribute less to bacterial virulence. The PCR detection of 2 GIs could be used as a cost effective and rapid tool to detect potentially virulent isolates that were contaminated in soil. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22675491/Genomic_islands_as_a_marker_to_differentiate_between_clinical_and_environmental_Burkholderia_pseudomallei_ L2 - https://dx.plos.org/10.1371/journal.pone.0037762 DB - PRIME DP - Unbound Medicine ER -