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Evaluation of compatibility and stability of filtered 99mTc-sulfur colloid when combined with fluorescent indocyanine green dye.
J Nucl Med Technol. 2012 Sep; 40(3):194-7.JN

Abstract

It is a common practice to administer dyes and radiopharmaceuticals separately for the localization of sentinel nodes in patients with biliary tract malignancies. The objective of this study was to evaluate the chemical properties and particle size of filtered (99m)Tc-sulfur colloid before and after it is combined with indocyanine green for injection. This study also evaluated the compatibility and stability of the two when combined, for the possibility of a single injection.

METHODS

(99m)Tc-sulfur colloid was prepared according to the package insert, and the final preparation was passed through a sterile 0.2-μm filter. Green dye was also prepared as per the package insert. In a sterile syringe, 0.25 mL of 14.8-MBq (400-μCi) filtered (99m)Tc-sulfur colloid was mixed with 0.25 mL of 1.25-mg green dye in a 1:1 proportion for a total volume of 0.50 mL. The radiochemical purity and pH of filtered (99m)Tc-sulfur colloid were obtained immediately and at 1 and 2 h after preparation. Particle size was analyzed using an electron microscope immediately and at 2 h.

RESULTS

The average radiochemical purity was 97.6% ± 2.0% (n = 51). The average pH was 5.56 ± 0.26 (n = 51). Evaluation of the particle size of filtered (99m)Tc-sulfur colloid with the green dye was determined by electron microscopy to be an average of 53 ± 30 nm (n = 365) at 0 h and 60 ± 35 nm (n = 303) at 2 h. This was compared with filtered (99m)Tc-sulfur colloid without the green dye, which averaged 71 ± 41 nm (n = 41). Measurements of unfiltered (99m)Tc-sulfur colloid were recorded at 253 ± 192 nm (n = 21) for additional comparisons.

CONCLUSION

The chemical properties and particle size of filtered (99m)Tc-sulfur colloid were not affected by the addition of the green dye; thus, combination of filtered (99m)Tc-sulfur colloid and green dye in the same syringe for administration is suitable.

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Authors+Show Affiliations

Hegseth KM
Division of Nuclear Medicine, Mayo Clinic, Rochester, Minnesota 55905-0001, USA.
Warren AM
No affiliation info available
Hung JC
No affiliation info available
Mosman EA
No affiliation info available

MeSH

Drug StabilityFiltrationFluorescent DyesHydrogen-Ion ConcentrationIndocyanine GreenParticle SizeTechnetium Tc 99m Sulfur Colloid

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22677616

Citation

Hegseth, Karissa M., et al. "Evaluation of Compatibility and Stability of Filtered 99mTc-sulfur Colloid when Combined With Fluorescent Indocyanine Green Dye." Journal of Nuclear Medicine Technology, vol. 40, no. 3, 2012, pp. 194-7.
Hegseth KM, Warren AM, Hung JC, et al. Evaluation of compatibility and stability of filtered 99mTc-sulfur colloid when combined with fluorescent indocyanine green dye. J Nucl Med Technol. 2012;40(3):194-7.
Hegseth, K. M., Warren, A. M., Hung, J. C., & Mosman, E. A. (2012). Evaluation of compatibility and stability of filtered 99mTc-sulfur colloid when combined with fluorescent indocyanine green dye. Journal of Nuclear Medicine Technology, 40(3), 194-7. https://doi.org/10.2967/jnmt.111.097584
Hegseth KM, et al. Evaluation of Compatibility and Stability of Filtered 99mTc-sulfur Colloid when Combined With Fluorescent Indocyanine Green Dye. J Nucl Med Technol. 2012;40(3):194-7. PubMed PMID: 22677616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of compatibility and stability of filtered 99mTc-sulfur colloid when combined with fluorescent indocyanine green dye. AU - Hegseth,Karissa M, AU - Warren,Amy M, AU - Hung,Joseph C, AU - Mosman,Elton A, Y1 - 2012/06/07/ PY - 2012/6/9/entrez PY - 2012/6/9/pubmed PY - 2013/7/3/medline SP - 194 EP - 7 JF - Journal of nuclear medicine technology JO - J Nucl Med Technol VL - 40 IS - 3 N2 - UNLABELLED: It is a common practice to administer dyes and radiopharmaceuticals separately for the localization of sentinel nodes in patients with biliary tract malignancies. The objective of this study was to evaluate the chemical properties and particle size of filtered (99m)Tc-sulfur colloid before and after it is combined with indocyanine green for injection. This study also evaluated the compatibility and stability of the two when combined, for the possibility of a single injection. METHODS: (99m)Tc-sulfur colloid was prepared according to the package insert, and the final preparation was passed through a sterile 0.2-μm filter. Green dye was also prepared as per the package insert. In a sterile syringe, 0.25 mL of 14.8-MBq (400-μCi) filtered (99m)Tc-sulfur colloid was mixed with 0.25 mL of 1.25-mg green dye in a 1:1 proportion for a total volume of 0.50 mL. The radiochemical purity and pH of filtered (99m)Tc-sulfur colloid were obtained immediately and at 1 and 2 h after preparation. Particle size was analyzed using an electron microscope immediately and at 2 h. RESULTS: The average radiochemical purity was 97.6% ± 2.0% (n = 51). The average pH was 5.56 ± 0.26 (n = 51). Evaluation of the particle size of filtered (99m)Tc-sulfur colloid with the green dye was determined by electron microscopy to be an average of 53 ± 30 nm (n = 365) at 0 h and 60 ± 35 nm (n = 303) at 2 h. This was compared with filtered (99m)Tc-sulfur colloid without the green dye, which averaged 71 ± 41 nm (n = 41). Measurements of unfiltered (99m)Tc-sulfur colloid were recorded at 253 ± 192 nm (n = 21) for additional comparisons. CONCLUSION: The chemical properties and particle size of filtered (99m)Tc-sulfur colloid were not affected by the addition of the green dye; thus, combination of filtered (99m)Tc-sulfur colloid and green dye in the same syringe for administration is suitable. SN - 1535-5675 UR - https://www.unboundmedicine.com/medline/citation/22677616/Evaluation_of_compatibility_and_stability_of_filtered_99mTc_sulfur_colloid_when_combined_with_fluorescent_indocyanine_green_dye_ L2 - http://tech.snmjournals.org/cgi/pmidlookup?view=long&pmid=22677616 DB - PRIME DP - Unbound Medicine ER -