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Structural characterisation and identification of phenylethanoid glycosides from Cistanches deserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS.
Phytochem Anal. 2012 Nov-Dec; 23(6):668-76.PA

Abstract

INTRODUCTION

Phenylethanoid glycosides (PhGs) are the major active constituents of Cistanches deserticola Y.C. Ma. However, the isolation, purification and identification procedures of PhGs are difficult and time-consuming.

OBJECTIVE

To establish a rapid and sensitive ultrahigh-pressure liquid chromatography (UHPLC)/ESI (electrospray ion source)-quadrupole time of flight (QTOF)-MS/MS method that could be applied to rapidly profile and identify PhGs.

METHODOLOGY

Seven standard compounds were used for the investigation of the fragmentation pattern. Based on the UHPLC/ESI-QTOF-MS/MS method, the important structural information on the types of aglycone and saccharide sequences present should be obtained.

RESULTS

According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 13 PhGs in the crude extract of C. deserticola were identified or tentatively identified.

CONCLUSION

A rapid and accurate UHPLC/ESI-QTOF-MS/MS method was established for the identification of PhGs in the crude extract of C. deserticola. This method therefore can be used for rapid prediction of the chemical constituents and qualities of this plant.

Authors+Show Affiliations

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin, 300193, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22678558

Citation

Han, Lifeng, et al. "Structural Characterisation and Identification of Phenylethanoid Glycosides From Cistanches Deserticola Y.C. Ma By UHPLC/ESI-QTOF-MS/MS." Phytochemical Analysis : PCA, vol. 23, no. 6, 2012, pp. 668-76.
Han L, Boakye-Yiadom M, Liu E, et al. Structural characterisation and identification of phenylethanoid glycosides from Cistanches deserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS. Phytochem Anal. 2012;23(6):668-76.
Han, L., Boakye-Yiadom, M., Liu, E., Zhang, Y., Li, W., Song, X., Fu, F., & Gao, X. (2012). Structural characterisation and identification of phenylethanoid glycosides from Cistanches deserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS. Phytochemical Analysis : PCA, 23(6), 668-76. https://doi.org/10.1002/pca.2371
Han L, et al. Structural Characterisation and Identification of Phenylethanoid Glycosides From Cistanches Deserticola Y.C. Ma By UHPLC/ESI-QTOF-MS/MS. Phytochem Anal. 2012 Nov-Dec;23(6):668-76. PubMed PMID: 22678558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterisation and identification of phenylethanoid glycosides from Cistanches deserticola Y.C. Ma by UHPLC/ESI-QTOF-MS/MS. AU - Han,Lifeng, AU - Boakye-Yiadom,Mavis, AU - Liu,Erwei, AU - Zhang,Yi, AU - Li,Wei, AU - Song,Xinbo, AU - Fu,Fenghua, AU - Gao,Xiumei, Y1 - 2012/06/07/ PY - 2011/12/23/received PY - 2012/03/26/revised PY - 2012/04/03/accepted PY - 2012/6/9/entrez PY - 2012/6/9/pubmed PY - 2013/4/27/medline SP - 668 EP - 76 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 23 IS - 6 N2 - INTRODUCTION: Phenylethanoid glycosides (PhGs) are the major active constituents of Cistanches deserticola Y.C. Ma. However, the isolation, purification and identification procedures of PhGs are difficult and time-consuming. OBJECTIVE: To establish a rapid and sensitive ultrahigh-pressure liquid chromatography (UHPLC)/ESI (electrospray ion source)-quadrupole time of flight (QTOF)-MS/MS method that could be applied to rapidly profile and identify PhGs. METHODOLOGY: Seven standard compounds were used for the investigation of the fragmentation pattern. Based on the UHPLC/ESI-QTOF-MS/MS method, the important structural information on the types of aglycone and saccharide sequences present should be obtained. RESULTS: According to the HPLC retention behaviour, the proposed fragmentation pathways provided by high-resolution MS and MS/MS spectra and literature sources, a total of 13 PhGs in the crude extract of C. deserticola were identified or tentatively identified. CONCLUSION: A rapid and accurate UHPLC/ESI-QTOF-MS/MS method was established for the identification of PhGs in the crude extract of C. deserticola. This method therefore can be used for rapid prediction of the chemical constituents and qualities of this plant. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/22678558/Structural_characterisation_and_identification_of_phenylethanoid_glycosides_from_Cistanches_deserticola_Y_C__Ma_by_UHPLC/ESI_QTOF_MS/MS_ L2 - https://doi.org/10.1002/pca.2371 DB - PRIME DP - Unbound Medicine ER -