Tags

Type your tag names separated by a space and hit enter

Differences in peripheral endocannabinoid modulation of scratching behavior in facial vs. spinally-innervated skin.
Neuropharmacology. 2012 Sep; 63(4):743-9.N

Abstract

Cannabinoids suppress nocifensive behaviors in rodents. We presently investigated peripheral endocannabinoid modulation of itch- and pain-related behaviors elicited from facial vs. spinally-innervated skin of rats. Intradermal (id) injection of the pruritogen serotonin (5-HT) elicited significantly more hindlimb scratch bouts, and longer cumulative time scratching, when injected in the rostral back compared to the cheek. Pretreatment of skin with inhibitors of degrading enzymes for the endocannabinoids anandamide (URB597) or 2-arachidonoylglycerol (JZL184) significantly reduced scratching elicited by 5-HT in the rostral back. These effects were prevented by co-treatment with antagonists of the CB₁ (AM251) or CB₂ receptor (AM630), implicating both receptor subtypes in endocannabinoid suppression of scratching in spinally-innervated skin. Conversely, pretreatment with either enzyme inhibitor, or with AM630 alone, increased the number of scratch bouts elicited by id 5-HT injection in the cheek. Moreover, pretreatment with JZL184 also significantly increased pain-related forelimb wipes directed to the cheek following id injection of the algogen, allyl isothiocyanate (AITC; mustard oil). Thus, peripheral endocannabinoids have opposite effects on itch-related scratching behaviors in trigeminally- vs. spinally-innervated skin. These results suggest that increasing peripheral endocannabinoid levels represents a promising therapeutic approach to treat itch arising from the lower body, but caution that such treatment may not relieve, and may even exacerbate, itch and pain arising from trigeminally-innervated skin of the face or scalp.

Authors+Show Affiliations

Department of Neurobiology, Physiology & Behavior, University of California, One Shields Avenue, Davis, CA 95616-8519, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

22683515

Citation

Spradley, Jessica Marie, et al. "Differences in Peripheral Endocannabinoid Modulation of Scratching Behavior in Facial Vs. Spinally-innervated Skin." Neuropharmacology, vol. 63, no. 4, 2012, pp. 743-9.
Spradley JM, Davoodi A, Gee LB, et al. Differences in peripheral endocannabinoid modulation of scratching behavior in facial vs. spinally-innervated skin. Neuropharmacology. 2012;63(4):743-9.
Spradley, J. M., Davoodi, A., Gee, L. B., Carstens, M. I., & Carstens, E. (2012). Differences in peripheral endocannabinoid modulation of scratching behavior in facial vs. spinally-innervated skin. Neuropharmacology, 63(4), 743-9. https://doi.org/10.1016/j.neuropharm.2012.05.032
Spradley JM, et al. Differences in Peripheral Endocannabinoid Modulation of Scratching Behavior in Facial Vs. Spinally-innervated Skin. Neuropharmacology. 2012;63(4):743-9. PubMed PMID: 22683515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differences in peripheral endocannabinoid modulation of scratching behavior in facial vs. spinally-innervated skin. AU - Spradley,Jessica Marie, AU - Davoodi,Auva, AU - Gee,Leland Bruce, AU - Carstens,Mirela Iodi, AU - Carstens,E, Y1 - 2012/06/06/ PY - 2012/01/22/received PY - 2012/05/09/revised PY - 2012/05/14/accepted PY - 2012/6/12/entrez PY - 2012/6/12/pubmed PY - 2012/12/10/medline SP - 743 EP - 9 JF - Neuropharmacology JO - Neuropharmacology VL - 63 IS - 4 N2 - Cannabinoids suppress nocifensive behaviors in rodents. We presently investigated peripheral endocannabinoid modulation of itch- and pain-related behaviors elicited from facial vs. spinally-innervated skin of rats. Intradermal (id) injection of the pruritogen serotonin (5-HT) elicited significantly more hindlimb scratch bouts, and longer cumulative time scratching, when injected in the rostral back compared to the cheek. Pretreatment of skin with inhibitors of degrading enzymes for the endocannabinoids anandamide (URB597) or 2-arachidonoylglycerol (JZL184) significantly reduced scratching elicited by 5-HT in the rostral back. These effects were prevented by co-treatment with antagonists of the CB₁ (AM251) or CB₂ receptor (AM630), implicating both receptor subtypes in endocannabinoid suppression of scratching in spinally-innervated skin. Conversely, pretreatment with either enzyme inhibitor, or with AM630 alone, increased the number of scratch bouts elicited by id 5-HT injection in the cheek. Moreover, pretreatment with JZL184 also significantly increased pain-related forelimb wipes directed to the cheek following id injection of the algogen, allyl isothiocyanate (AITC; mustard oil). Thus, peripheral endocannabinoids have opposite effects on itch-related scratching behaviors in trigeminally- vs. spinally-innervated skin. These results suggest that increasing peripheral endocannabinoid levels represents a promising therapeutic approach to treat itch arising from the lower body, but caution that such treatment may not relieve, and may even exacerbate, itch and pain arising from trigeminally-innervated skin of the face or scalp. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/22683515/Differences_in_peripheral_endocannabinoid_modulation_of_scratching_behavior_in_facial_vs__spinally_innervated_skin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(12)00230-4 DB - PRIME DP - Unbound Medicine ER -